Carbazole ligands as c-myc G-quadruplex binders

The interactions of c-myc G-quadruplex with three carbazole derivatives were investigated by UV-Vis spectrophotometry, fluorescence, CD spectroscopy, and molecular modeling. The results showed that a combination of carbazole scaffold functionalized with ethyl, triazole and imidazole groups resulted in stabilization of the intramolecular G-quadruplex formed by the DNA sequence derived from the NHE III1 region of c-myc oncogene (Pu22). Binding to the G-quadruplex Pu22 resulted in the significant increase in fluorescence intensity of complexed ligands 1-3. All ligands were capable of interacting with G4 DNA with binding stoichiometry indicating that two ligand molecules bind to G-quadruplex with comparable affinity, which agrees with binding model of end-stacking on terminal G-tetrads

Florida\u27s Mystery Coral-Killer Identified

An unusual coral disease appeared on the Florida Reef Tract in June 1995. It was distinct in its microbiology, its pattern of tissue degradation, the species susceptible to it, and its regional distribution. Symptoms included a sharp line between healthy and diseased tissue, as occurs with other coral diseases, but the pathogen responsible for the new outbreak seemed more virulent, affected a wider variety of species, and destroyed tissue much more rapidly than these other \u27line\u27 or \u27band\u27 diseases. We have identified the pathogen responsible for this new disease as a new species of Sphingomonas

Spatio-Temporal Transmission Patterns of Black-Band Disease in a Coral Community

Transmission mechanisms of black-band disease (BBD) in coral reefs are poorly understood, although this disease is considered to be one of the most widespread and destructive coral infectious diseases. The major objective of this study was to assess transmission mechanisms of BBD in the field based on the spatio-temporal patterns of the disease

Global profiling of co- and post-translationally N-myristoylated proteomes in human cells

Protein N-myristoylation is a ubiquitous co- and post-translational modification that has been implicated in the development and progression of a range of human diseases. Here, we report the global N-myristoylated proteome in human cells determined using quantitative chemical proteomics combined with potent and specific human N-myristoyltransferase (NMT) inhibition. Global quantification of N-myristoylation during normal growth or apoptosis allowed the identification of >100 N-myristoylated proteins, >95% of which are identified for the first time at endogenous levels. Furthermore, quantitative dose response for inhibition of N-myristoylation is determined for >70 substrates simultaneously across the proteome. Small-molecule inhibition through a conserved substrate-binding pocket is also demonstrated by solving the crystal structures of inhibitor-bound NMT1 and NMT2. The presented data substantially expand the known repertoire of co- and post-translational N-myristoylation in addition to validating tools for the pharmacological inhibition of NMT in living cells

Cytotaxonomic studies on some Vicia L. species growing in the eastern Mediterranean and southern Aegean regions II.

In this study, some natural Vicia L. species growing naturally in eastern Mediterranean and southern Aegean Regions have been investigated morphological and cytotaxonomic point of view. Morphology, chromosome number and morphometries of the 9 taxa belonging to the Vicia L. genus including Vicia peregrina, V. lutea var. hirta, V. anatolica, V. hybrida, V. grandiflora var. grandiflora, V. grandiflora var. dissecta, V. cuspidata, V. sativa subsp. sativa, V. sativa subsp. nigra var. segatalis have been studied by using karyological and numerical taxonomic techniques. Data obtained chromosome measurements were analysed by using cluster analysis. The chromosome number of studied Vicia species have been found as 2n = 10, 12 and 14. The member of Vicia section, related taxa were compared with respect to their chromosomal and morphological characteristics. Some karyological relationships among the studied Vicia taxa were discussed with help of taxonomic history and evolutionary data

Traditional media practitioners\' perception of entertainment-education utilization for agricultural information dissessmination in Nigeria

This study investigated traditional media practitioners' perception of entertainment–education (EE) utilization for dissemination of agricultural information. One hundred and two respondents were sampled across music and theatre groups in south west Nigeria through multi – stage sampling technique. The result reveals that 89.4% of respondents with different educational qualifications and 80.3% of varied years of professionals experience favourably perceived EE format utilization for agricultural information dissemination. However, traditional media practitioners of different educational qualifications (73.6%), statuses (76.5%) unanimously opined that perceived constraints to EE utilization are very severe. The result further indicates that there is a significant relationship between educational qualification (X2 = 6.21; p 0.05) and medium (X2 = 2.12; P > 0.05) and practitioners' disposition to the format utilization. Music and theatre practitioners also differ in their overall perception of EE format utilization for agricultural information dissemination. Therefore, given the overall favourable disposition of practitioners to EE format utilization, it is recommended that traditional media should be integrated into development interventions using EE strategy for its potency in information dissemination. Key words: Traditional media; entertainment-education; agricultural information dissemination and perception. Moor Journal of Agricultural Research Vol.4(2) 2003: 266-27

The impact of agriculture on environmental health in Nigeria.

The paper assessed the impact of agriculture on environmental health in Nigeria. The paper reviewed sources of environmental degradation as a result of agriculture practices that has direct link with soil erosion, desert encroachment and unabated deforestation as well as poor soil conservation methods. Evidences from various sites reveal that the use of antibiotics in animal farming and insecticides accounts for high incidences of food poisoning and deaths of unsuspecting consumers especially the non-literate farmers in rural areas. This is particularly common in aquaculture where such chemicals have been used extensively to catch fish. The situation is further worsened due to administrative and poor regulatory and enforcement system. Both the terrestrial and adequate habitats have been endangered by crops in agriculture enterprise which had degraded the environment via soil exhaustion and unregulated use of herbicides, pesticides, fertilizers and other chemicals, which is further complicated by the unguarded pattern of food processing. Land denudation, aquatic toxicity; leaching, run off and lifetime health equivalent are some of the complicated environmental hazard and health menace that both agricultural activities and food processing can cause the environment and the occupants. Consequently, pragmatic suggestions are made on how to avert the imminent environmental catastrophe through comprehensive environmental extension education, mass awareness and strict enforcement of environmental regulations. Key words: Agriculture, environment, health and extension [JEXT Vol.3 2002: 47-58