Simultaneous Determination of Selected Flavonoids from Different Cistus Species by Hplc-Pda

Scopu

In Vitro and in Silico Investigation of DNA Interaction, Topoisomerase I and II Inhibitory Properties of Polydatin

Collaboration between: Hacettepe University, Faculty of Pharmacy, Department of Pharmacognosy, Sıhhiye, Ankara, Turkey Karadeniz Technical University, Faculty of Pharmacy, Department of Biochemistry, Trabzon, Turkey Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Sıhhiye, Ankara, Turkey Karadeniz Technical University, Drug and Pharmaceutical Technology Application and Research Center, Trabzon, Turkey De Montfort University, Leicester School of Pharmacy, The Gateway, Leicester, LE1 9BH, United Kingdom The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.Polydatin or piceid, is the 3-O-glucoside of resveratrol and is found abundantly in grapes, peanuts, wine, beer, and cacao products. Although anti-cancer-activity of polydatin was reported before, and potential antiproliferative mechanisms of polydatin have been proposed, its direct effects on DNA and inhibitory potential against topoisomerase enzymes have remained unknown. In this study we aimed to reveal the link between polydatin’s effects on DNA and DNA-topoisomerases and its antiproliferative promise. For this purpose, we evaluated the effects of polydatin on DNA and DNA topoisomerase using in vitro and in silico techniques. Polydatin was found to protect DNA against Fenton reaction-induced damage while not showing any hydrolytic nuclease effect. Further, polydatin inhibited topoisomerase II but not topoisomerase I. According to molecular docking studies, polydatin preferably showed minor groove binding to DNA where the stilbene moiety was important for binding to the DNA-topoisomerase II complex. As a result, topoisomerase II inhibition might be another anti-cancer mechanism of polydatin

The evaluation of wound healing potential of rosmarinic acid isolated from Arnebia purpurea

63rd International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA) -- AUG 23-27, 2015 -- Budapest, HUNGARY[No Abstract Available

β-Hydroxydihydrochalcone and flavonoid glycosides along with triterpene saponin and sesquiterpene from the herbs of <i>Pimpinella rhodantha</i> Boiss.

<p>A new β-hydroxydihydrochalcone glycoside named ziganin (<b>1</b>) and a new acylated flavonol glycoside named isorhamnetin-3-<i>O-</i>α-L-(2″,3″-di-<i>O-trans</i>-coumaroyl)-rhamnopyranoside) (<b>2</b>), along with two known flavonoid glycosides, a β-hydroxydihydrochalcone glycoside, a hydroxybenzoic acid derivative, a trinorguaiane type sesquiterpenoid, a triterpenic saponin and a polyol were isolated from the herbs of <i>Pimpinella rhodantha</i> Boiss. Their structures were elucidated on the basis of spectroscopic analyses including 1D-and 2D-NMR, UV, IR, CD, ESI-MS, APCI-MS, HR-ESI-MS techniques. The isolated compounds were evaluated for their antioxidant capacity through the DPPH free-radical scavenging assay and ferrous ion-chelating power test.</p

The effect of Arnebia purpurea extract on the survival of random pattern skin flaps in rats

Objectives. One of the important causes of flap losses is ischemia-reperfusion damage. Arnebia species are rich in naphthoquinone derivatives which known to have anti-inflammatory and anti-oxidant effects. The aim of the present study was to investigate the effects of Arnebia purpurea extract on viability of the skin flaps. Methods. Eighteen Wistar rats were divided in three groups. Caudal-based 9×3 cm size skin flap was applied on dorsum of the all rats and following surgery, 2 cc of A. purpurea extract topically was applied to the group 1 and 2 cc of 0.2% Nitrofurazon cream topically was applied to the group 2, daily. The Group 3 only received flap surgery (control group). Seven days later, all subjects were euthanatized and necrosis rate in their flaps was calculated and compared to each other. Results. The necrosis rate was calculated as 20.25% ± 1.59% in Group 1, 32.05% ± 2.23% in the Group 2 and 37.33% ± 4.12% in the Group 3. It was found that necrosis rate in the Group 1 was statistically significantly less than Groups 2 and 3 (p 0.05). Conclusion. Necrosis of skin flaps was reduced through topical application of A. purpurea extract