Slip distribution and tectonic implication of the 1999 Chi‐Chi, Taiwan, Earthquake

We report on the fault complexity of the large (M_w = 7.6) Chi‐Chi earthquake obtained by inverting densely and well‐distributed static measurements consisting of 119 GPS and 23 doubly integrated strong motion records. We show that the slip of the Chi-Chi earthquake was concentrated on the surface of a ”wedge shaped” block. The inferred geometric complexity explains the difference between the strike of the fault plane determined by long period seismic data and surface break observations. When combined with other geophysical and geological observations, the result provides a unique snapshot of tectonic deformation taking place in the form of very large (>10m) displacements of a massive wedge‐shaped crustal block which may relate to the changeover from over‐thrusting to subducting motion between the Philippine Sea and the Eurasian plates

Slip history and dynamic implications of the 1999 Chi-Chi, Taiwan, earthquake

We investigate the rupture process of the 1999 Chi-Chi, Taiwan, earthquake using extensive near-source observations, including three-component velocity waveforms at 36 strong motion stations and 119 GPS measurements. A three-plane fault geometry derived from our previous inversion using only static data [ Ji et al., 2001 ] is applied. The slip amplitude, rake angle, rupture initiation time, and risetime function are inverted simultaneously with a recently developed finite fault inverse method that combines a wavelet transform approach with a simulated annealing algorithm [ Ji et al., 2002b ]. The inversion results are validated by the forward prediction of an independent data set, the teleseismic P and SH ground velocities, with notable agreement. The results show that the total seismic moment release of this earthquake is 2.7 × 10^20 N m and that most of the slip occurred in a triangular-shaped asperity involving two fault segments, which is consistent with our previous static inversion. The rupture front propagates with an average rupture velocity of ∼2.0 km s^(−1), and the average slip duration (risetime) is 7.2 s. Several interesting observations related to the temporal evolution of the Chi-Chi earthquake are also investigated, including (1) the strong effect of the sinuous fault plane of the Chelungpu fault on spatial and temporal variations in slip history, (2) the intersection of fault 1 and fault 2 not being a strong impediment to the rupture propagation, and (3) the observation that the peak slip velocity near the surface is, in general, higher than on the deeper portion of the fault plane, as predicted by dynamic modeling

Metamorphic testing for cybersecurity

Metamorphic testing (MT) can enhance security testing by providing an alternative to using a testing oracle, which is often unavailable or impractical. The authors report how MT detected previously unknown bugs in real-world critical applications such as code obfuscators, giving evidence that software testing requires diverse perspectives to achieve greater cybersecurity

Modulating Microglia/Macrophage Activation by CDNF Promotes Transplantation of Fetal Ventral Mesencephalic Graft Survival and Function in a Hemiparkinsonian Rat Model

Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons in substantia nigra pars compacta, which leads to the motor control deficits. Recently, cell transplantation is a cutting-edge technique for the therapy of PD. Nevertheless, one key bottleneck to realizing such potential is allogenic immune reaction of tissue grafts by recipients. Cerebral dopamine neurotrophic factor (CDNF) was shown to possess immune-modulatory properties that benefit neurodegenerative diseases. We hypothesized that co-administration of CDNF with fetal ventral mesencephalic (VM) tissue can improve the success of VM replacement therapies by attenuating immune responses. Hemiparkinsonian rats were generated by injecting 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle of Sprague Dawley (SD) rats. The rats were then intrastriatally transplanted with VM tissue from rats, with/without CDNF administration. Recovery of dopaminergic function and survival of the grafts were evaluated using the apomorphine-induced rotation test and smallanimal positron emission tomography (PET) coupled with [F-18] DOPA or [F-18] FE-PE2I, respectively. In addition, transplantation-related inflammatory response was determined by uptake of [F-18] FEPPA in the grafted side of striatum. Immunohistochemistry (IHC) examination was used to determine the survival of the grated dopaminergic neurons in the striatum and to investigate immune-modulatory effects of CDNF. The modulation of inflammatory responses caused by CDNF might involve enhancing M2 subset polarization and increasing fractal dimensions of 6-OHDA-treated BV2 microglial cell line. Analysis of CDNF-induced changes to gene expressions of 6-OHDA-stimulated BV2 cells implies that these alternations of the biomarkers and microglial morphology are implicated in the upregulation of protein kinase B signaling as well as regulation of catalytic, transferase, and protein serine/threonine kinase activity. The effects of CDNF on 6-OHDA-induced alternation of the canonical pathway in BV2 microglial cells is highly associated with PI3K-mediated phagosome formation. Our results are the first to show that CDNF administration enhances the survival of the grafted dopaminergic neurons and improves functional recovery in PD animal model. Modulation of the polarization, morphological characteristics, and transcriptional profiles of 6-OHDA-stimualted microglia by CDNF may possess these properties in transplantation-based regenerative therapies.Peer reviewe

Modulating Microglia/Macrophage Activation by CDNF Promotes Transplantation of Fetal Ventral Mesencephalic Graft Survival and Function in a Hemiparkinsonian Rat Model

Parkinson’s disease (PD) is characterized by the loss of dopaminergic neurons in substantia nigra pars compacta, which leads to the motor control deficits. Recently, cell transplantation is a cutting-edge technique for the therapy of PD. Nevertheless, one key bottleneck to realizing such potential is allogenic immune reaction of tissue grafts by recipients. Cerebral dopamine neurotrophic factor (CDNF) was shown to possess immune-modulatory properties that benefit neurodegenerative diseases. We hypothesized that co-administration of CDNF with fetal ventral mesencephalic (VM) tissue can improve the success of VM replacement therapies by attenuating immune responses. Hemiparkinsonian rats were generated by injecting 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle of Sprague Dawley (SD) rats. The rats were then intrastriatally transplanted with VM tissue from rats, with/without CDNF administration. Recovery of dopaminergic function and survival of the grafts were evaluated using the apomorphine-induced rotation test and small-animal positron emission tomography (PET) coupled with [18F] DOPA or [18F] FE-PE2I, respectively. In addition, transplantation-related inflammatory response was determined by uptake of [18F] FEPPA in the grafted side of striatum. Immunohistochemistry (IHC) examination was used to determine the survival of the grated dopaminergic neurons in the striatum and to investigate immune-modulatory effects of CDNF. The modulation of inflammatory responses caused by CDNF might involve enhancing M2 subset polarization and increasing fractal dimensions of 6-OHDA-treated BV2 microglial cell line. Analysis of CDNF-induced changes to gene expressions of 6-OHDA-stimulated BV2 cells implies that these alternations of the biomarkers and microglial morphology are implicated in the upregulation of protein kinase B signaling as well as regulation of catalytic, transferase, and protein serine/threonine kinase activity. The effects of CDNF on 6-OHDA-induced alternation of the canonical pathway in BV2 microglial cells is highly associated with PI3K-mediated phagosome formation. Our results are the first to show that CDNF administration enhances the survival of the grafted dopaminergic neurons and improves functional recovery in PD animal model. Modulation of the polarization, morphological characteristics, and transcriptional profiles of 6-OHDA-stimualted microglia by CDNF may possess these properties in transplantation-based regenerative therapies

Evidence for fault lubrication during the 1999 Chi-Chi, Taiwan, earthquake (Mw7.6)

The ground motion data of the 1999 Chi-Chi, Taiwan, earthquake exhibit a striking difference in frequency content between the north and south portions of the rupture zone. In the north, the ground motion is dominated by large low-frequency displacements with relatively small high-frequency accelerations. The pattern is opposite in the south, with smaller displacements and larger accelerations. We analyze the fault dynamics in light of a fault lubrication mechanism using near-field seismograms and a detailed rupture model. The fault zone contains viscous material (e.g., gouge), in which pressure increases following the Reynolds lubrication equation. When the displacement exceeds a threshold, lubrication pressure becomes high enough to widen the gap, thereby reducing the area of asperity contact. With less asperity contact, the fault slips more smoothly, suppressing high-frequency radiation

Improving thermal stability and efficacy of BCNU in treating glioma cells using PAA-functionalized graphene oxide

Yu-Jen Lu1,2,#, Hung-Wei Yang1,#, Sheng-Che Hung3, Chiung-Yin Huang2, Shin-Ming Li4, Chen-Chi M Ma4, Pin-Yuan Chen2, Hong-Chieh Tsai2, Kuo-Chen Wei2, Jyh-Ping Chen1 1Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan, Taiwan; 2Department of Neurosurgery, Chang Gung Memorial Hospital, Kwei-San, Taoyuan, Taiwan; 3Department of Radiology, Taipei Veterans General Hospital, Taipei, Taiwan; 4Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan#These authors contributed equally to this workBackground: 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), a commercial chemotherapeutic drug for treating malignant brain tumors, has poor thermal stability and a short half-life. Immobilization of BCNU on a nanocarrier might increase the thermal stability of BCNU and extend its half-life.Methods: Nanosized graphene oxide (GO) could be modified by polyacrylic acid (PAA) to improve the aqueous solubility and increase the cell penetration efficacy of the nanocarrier. PAA–GO intended as a drug carrier for BCNU was prepared and characterized in this study. The size and thickness of PAA–GO was investigated by transmission electron microscopy and atomic force microscopy, and the presence of PAA functional groups was confirmed by electron spectroscopy for chemical analysis and thermogravimetric analysis. BCNU was conjugated to PAA–GO by covalent binding for specific killing of cancer cells, which could also enhance the thermal stability of the drug.Results: Single layer PAA–GO (about 1.9 nm) with a lateral width as small as 36 nm was successfully prepared. The optimum drug immobilization condition was by reacting 0.5 mg PAA–GO with 0.4 mg BCNU, and the drug-loading capacity and residual drug activity were 198 µg BCNU/mg PAA–GO and 70%, respectively. This nanocarrier significantly prolonged the half-life of bound BCNU from 19 to 43 hours compared with free drug and showed efficient intracellular uptake by GL261 cancer cells. The in vitro anticancer efficacy of PAA–GO–BCNU was demonstrated by a 30% increase in DNA interstrand cross-linking and a 77% decrease in the IC50 value toward GL261 compared with the same dosage of free drug.Conclusion: Nanosized PAA–GO serves as an efficient BCNU nanocarrier by covalent binding. This nanocarrier will be a promising new vehicle for an advanced drug delivery system in cancer therapy.Keywords: graphene oxide, BCNU, glioma cells, drug delivery, thermal stabilit

Acquiring Authentic Data in Unattended Wireless Sensor Networks

An Unattended Wireless Sensor Network (UWSN) can be used in many applications to collect valuable data. Nevertheless, due to the unattended nature, the sensors could be compromised and the sensor readings would be maliciously altered so that the sink accepts the falsified sensor readings. Unfortunately, few attentions have been given to this authentication problem. Moreover, existing methods suffer from different kinds of DoS attacks such as Path-Based DoS (PDoS) and False Endorsement-based DoS (FEDoS) attacks. In this paper, a scheme, called AAD, is proposed to Acquire Authentic Data in UWSNs. We exploit the collaboration among sensors to address the authentication problem. With the proper design of the collaboration mechanism, AAD has superior resilience against sensor compromises, PDoS attack, and FEDoS attack. In addition, compared with prior works, AAD also has relatively low energy consumption. In particular, according to our simulation, in a network with 1,000 sensors, the energy consumed by AAD is lower than 30% of that consumed by the existing method, ExCo. The analysis and simulation are also conducted to demonstrate the superiority of the proposed AAD scheme over the existing methods