Aktivitas Antiproliferatif Ekstrak Wasbensin Daun Eupatorium Riparium Reg. : Studi in Vitro Pada HeLa Cell Lin

Eupatorium riparium Reg. adalah tumbuhan obat penting asli dari Mexico dan India Barat, yang masuk ke tanah Jawa sejak tahun 1800. Tumbuhan ini mempunyai catatan sejarah digunakan untuk obat tradisional dalam berbagai kultur budaya bangsa secara luas di seluruh dunia dan biasa digunakan untuk obat hipertensi, gagal jantung, diuretik, antikanker, antifungi, dan penyakit yang disebabkan oleh bakteri. Studi pada HeLa cell line ini dilakukan selama satu bulan. Selanjutnya, studi ini bertujuan meneliti aktivitas antiproliferatif ekstrak wasbensin daun E. riparium terhadap kanker servik manusia Hela cell line. Aktivitas antiproliferatif diuji menggunakan reagen proliferatif sel WST-1 dengan waktu 1, 2, dan 4 jam setelah diinkubasi selama 72 jam pada suhu 37oC dan 5%CO2. Hasil penelitian menunjukkan bahwa ekstrak wasbensin daun E. riparium mempunyai aktivitas proliferatif yang potensial terhadap HeLa cell line dengan nilai IC50 berikut 102.69 ðœ‡g/ml (1 jam), 198.67 ðœ‡g/ml (2 jam). Saran selanjutnya, penelitian lanjutan perlu dilakukan untuk mengetahui mekanisme antikanker HeLa cell line

Aktivitas Antiproliferatif Ekstrak Wasbensin Daun Eupatorium riparium Reg. : Studi In Vitro Pada HeLa Cell lin

Eupatorium riparium Reg. adalah tumbuhan obat penting asli dari Mexico dan India Barat, yang masuk ke tanah Jawa sejak tahun 1800. Tumbuhan ini mempunyai catatan sejarah digunakan untuk obat tradisional dalam berbagai kultur budaya bangsa secara luas di seluruh dunia dan biasa digunakan untuk obat hipertensi, gagal jantung, diuretik, antikanker, antifungi, dan penyakit yang disebabkan oleh bakteri. Studi pada HeLa cell line ini dilakukan selama satu bulan. Selanjutnya, studi ini bertujuan meneliti aktivitas antiproliferatif ekstrak wasbensin daun E. riparium terhadap kanker servik manusia Hela cell line. Aktivitas antiproliferatif diuji menggunakan reagen proliferatif sel WST-1 dengan waktu 1, 2, dan 4 jam setelah diinkubasi selama 72 jam pada suhu 37oC dan 5%CO2. Hasil penelitian menunjukkan bahwa ekstrak wasbensin daun E. riparium mempunyai aktivitas proliferatif yang potensial terhadap HeLa cell line dengan nilai IC50 berikut 102.69 ðœ‡g/ml (1 jam), 198.67 ðœ‡g/ml (2 jam). Saran selanjutnya, penelitian lanjutan perlu dilakukan untuk mengetahui mekanisme antikanker HeLa cell line.Kata kunci: Eupatorium riparium Reg, antiproliferatif, HeLa, WST-

大腿四頭筋の超音波画像を用いた等尺性膝関節伸展筋力の推定

Muscle echo intensity (EI) determined by ultrasonography has recently been reported asan index of intramuscular fat. The muscle thickness (MT) and EI of the quadriceps femorismuscle were reported to be influenced by the muscle strength (MS) of the knee extensors.This study was performed to investigate whether the MT and EI of the quadriceps femorisare associated with the MS of knee extensors, and to establish a predictive formula for themaximal isometric MS of knee extensors.Forty healthy volunteers 20-59 years old were included in this study. The maximalisometric MS of knee extensors on the dominant extremity was measured at kneeflexion of 60°, and was defined as the maximal value over three repeated measurements.Transverse ultrasound images of the quadriceps on the dominant extremity were obtainedwith a B-mode ultrasound imaging device and multi-frequency linear transducer. Duringmeasurements, the participants were completely relaxed and sat comfortably withthe knee flexed at 90°. A 10-MHz transducer with gain of 58 dB was used during allmeasurements. The transducer was positioned perpendicular to the longitudinal axis ofthe quadriceps femoris, at the midpoint between the anterior superior iliac spine andthe proximal end of the patella. The same investigator then obtained three consecutiveimages. The subcutaneous fat thickness, MT of each muscle, and EI of each muscle wereanalyzed from three images acquired for the rectus femoris (RF) and vastus intermedius(VI), and the mean values of the three measurements were recorded. Pearson’s andSpearman’s correlation coefficients were calculated to investigate the relationships betweensubcutaneous fat thickness, MT, EI, physical characteristics, and MS. Stepwise multipleregression analysis was then performed with MS value as the dependent variable, and thecharacteristics of the participants and values from the ultrasound images as independentvariables. In addition, multiple regression analysis was performed in two groups dividedaccording to age, i.e., the young adult group and the middle-aged group.There were no significant differences in height, weight, BMI, or muscle circumferencebetween the young adult group and middle-aged group. MS showed significant negativecorrelations with subcutaneous fat thickness, RFEI, and VIEI, and significant positivecorrelations with height, weight, circumference, RFMT, and VIMT. Stepwise regressionanalysis identified height and VIMT as factors significantly associated with MS (adjustedR2 = 0.65). Height and VIMT contributed to MS in young and middle-aged individuals

Inhibitory Effects of Ecklonia cava Extract on High Glucose-Induced Hepatic Stellate Cell Activation

Nonalcoholic steatohepatitis (NASH) is a disease closely associated with obesity and diabetes. A prevalence of type 2 diabetes and a high body mass index in cryptogenic cirrhosis may imply that obesity leads to cirrhosis. Here, we examined the effects of an extract of Ecklonia cava, a brown algae, on the activation of high glucose-induced hepatic stellate cells (HSCs), key players in hepatic fibrosis. Isolated HSCs were incubated with or without a high glucose concentration. Ecklonia cava extract (ECE) was added to the culture simultaneously with the high glucose. Treatment with high glucose stimulated expression of type I collagen and α-smooth muscle actin, which are markers of activation in HSCs, in a dose-dependent manner. The activation of high glucose-treated HSCs was suppressed by the ECE. An increase in the formation of intracellular reactive oxygen species (ROS) and a decrease in intracellular glutathione levels were observed soon after treatment with high glucose, and these changes were suppressed by the simultaneous addition of ECE. High glucose levels stimulated the secretion of bioactive transforming growth factor-β (TGF-β) from the cells, and the stimulation was also suppressed by treating the HSCs with ECE. These results suggest that the suppression of high glucose-induced HSC activation by ECE is mediated through the inhibition of ROS and/or GSH and the downregulation of TGF-β secretion. ECE is useful for preventing the development of diabetic liver fibrosis