The Effect of High Intensity Focused Ultrasound on Enterococcus Faecalis Biofilm

Master'sMASTER OF SCIENC

تحليل الأخطاء الإملائية في كتابة الهمزة لطلاب الفصل الثالث المتوسّط بمعهد دار القلم جينتونج جايانتي تانجرانج

يهدف هذا البحث إلى معرفة الأخطاء الإملائية في كتابة الهمزة لطلاب الفصل الثالث المتوسّط بمعهد دار القلم و معرفة أسبابها باستخدام منهج تحليل الأخطاء. و هذا البحث هو البحث الكيفي. واستخدم البحث الميدني من حيث جمع البيانات الأساسيّة من كتابة الطلبة و المقابلة معهم، والبيانات الثانوية فمصدرها من الكتب المتعلقة بتحليل الأخطاء الإملائية في كتابة الهمزة. الأخطاء في كتابة الهمزة كلها ثلاث مائة و سبعة و ستّون خطأً. و أشكال الأخطاء الإملائيّة في كتابة الهمزة لطلّاب الفصل الثالث  المتوسط بمعهد دار القلم تتشكّل من الأخطاء في كتابة الهمزة الابتدائيّة تبلغ ثلاثة مائة وستّة عشر خطأً، أي 86,10%  (أي همزة الوصل مائتان و خمسة عشر خطأ أو 58,58%. و همزة القطع مائة و واحد خطأً أو 27,52%) و الأخطاء في كتابة الهمزة وسط الكلمة أربعون خطأً أو 10,89% و كتابة الهمزة آخر الكلمة تسعة أخطاء أو 2,45%. أمّا أسباب الأخطاء فهي النسيان عن القاعدة، و الخطأ في سمع الكلمة، و نقصان التعمّق في درس اللغة العربيّة، و عدم معرفة القاعدة، و التعوّد على الكتابة بطريقة خاطئة، و الاستعجال في الكتابة، و الامتثال إلى جواب رفيقه الصّفّ الخاطئ، و كون الكلمة مجهولة لدى الطّلّاب، و كون الكلمة غير المستخدمة في محادثة الطّلّاب اليوميّة

Ta?l?l al-akhth?' al-iml?'iyyah f? kit?bah al-hamzah li thul?b al-fashl al-ts?lits al-mutawassith bi ma'had d?r al-qalam gintung jayanti tangerang (dir?sah ta?l?l al-akhth?')

190 hl

Ta?l?l al-akhth?' al-iml?'iyyah f? kit?bah al-hamzah li thul?b al-fashl al-ts?lits al-mutawassith bi ma'had d?r al-qalam gintung jayanti tangerang (dir?sah ta?l?l al-akhth?')

190 hl

Asymptomatic COVID-19 Presenting with Features of Mixed Pattern Acute Liver Injury in a Young Healthy Female, a Case Report

COVID-19 associated severe acute liver injury in a young healthy patient has not been reported much in the literature. And currently, there are no standard management guidelines. We want to report a case of acute liver injury of mixed pattern in a young healthy female with asymptomatic COVID-19 infection. She presented with abdominal pain, nausea, vomiting and yellowish discoloration of her skin. Further laboratory investigations revealed mixed pattern liver injury with highly raised liver enzymes. She was managed with N-acetyl cysteine protocol and monitoring of her liver enzymes. Other causes of acute liver injury were ruled out. She remained stable during her hospital stay and follow up. Our aim is to highlight the significance of acute liver injury in COVID 19 patients that may lead to fatal outcomes if not managed and monitored accordingly

Lineage-specific differentiation of osteogenic progenitors from pluripotent stem cells reveals the FGF1-RUNX2 association in neural crest-derived osteoprogenitors.

Human pluripotent stem cells (hPSCs) can provide a platform to model bone organogenesis and disease. To reflect the developmental process of the human skeleton, hPSC differentiation methods should include osteogenic progenitors (OPs) arising from three distinct embryonic lineages: the paraxial mesoderm, lateral plate mesoderm, and neural crest. Although OP differentiation protocols have been developed, the lineage from which they are derived, as well as characterization of their genetic and molecular differences, has not been well reported. Therefore, to generate lineage-specific OPs from human embryonic stem cells and human induced pluripotent stem cells, we employed stepwise differentiation of paraxial mesoderm-like cells, lateral plate mesoderm-like cells, and neural crest-like cells toward their respective OP subpopulation. Successful differentiation, confirmed through gene expression and in vivo assays, permitted the identification of transcriptomic signatures of all three cell populations. We also report, for the first time, high FGF1 levels in neural crest-derived OPs-a notable finding given the critical role of fibroblast growth factors (FGFs) in osteogenesis and mineral homeostasis. Our results indicate that FGF1 influences RUNX2 levels, with concomitant changes in ERK1/2 signaling. Overall, our study further validates hPSCs' power to model bone development and disease and reveals new, potentially important pathways influencing these processes

Lineage-specific differentiation of osteogenic progenitors from pluripotent stem cells reveals the FGF1-RUNX2 association in neural crest-derived osteoprogenitors.

Human pluripotent stem cells (hPSCs) can provide a platform to model bone organogenesis and disease. To reflect the developmental process of the human skeleton, hPSC differentiation methods should include osteogenic progenitors (OPs) arising from three distinct embryonic lineages: the paraxial mesoderm, lateral plate mesoderm, and neural crest. Although OP differentiation protocols have been developed, the lineage from which they are derived, as well as characterization of their genetic and molecular differences, has not been well reported. Therefore, to generate lineage-specific OPs from human embryonic stem cells and human induced pluripotent stem cells, we employed stepwise differentiation of paraxial mesoderm-like cells, lateral plate mesoderm-like cells, and neural crest-like cells toward their respective OP subpopulation. Successful differentiation, confirmed through gene expression and in vivo assays, permitted the identification of transcriptomic signatures of all three cell populations. We also report, for the first time, high FGF1 levels in neural crest-derived OPs-a notable finding given the critical role of fibroblast growth factors (FGFs) in osteogenesis and mineral homeostasis. Our results indicate that FGF1 influences RUNX2 levels, with concomitant changes in ERK1/2 signaling. Overall, our study further validates hPSCs' power to model bone development and disease and reveals new, potentially important pathways influencing these processes

Quantitative Craniofacial Analysis and Generation of Human Induced Pluripotent Stem Cells for Muenke Syndrome: A Case Report

In this case report, we focus on Muenke syndrome (MS), a disease caused by the p.Pro250Arg variant in fibroblast growth factor receptor 3 (FGFR3) and characterized by uni- or bilateral coronal suture synostosis, macrocephaly without craniosynostosis, dysmorphic craniofacial features, and dental malocclusion. The clinical findings of MS are further complicated by variable expression of phenotypic traits and incomplete penetrance. As such, unraveling the mechanisms behind MS will require a comprehensive and systematic way of phenotyping patients to precisely identify the impact of the mutation variant on craniofacial development. To establish this framework, we quantitatively delineated the craniofacial phenotype of an individual with MS and compared this to his unaffected parents using three-dimensional cephalometric analysis of cone beam computed tomography scans and geometric morphometric analysis, in addition to an extensive clinical evaluation. Secondly, given the utility of human induced pluripotent stem cells (hiPSCs) as a patient-specific investigative tool, we also generated the first hiPSCs derived from a family trio, the proband and his unaffected parents as controls, with detailed characterization of all cell lines. This report provides a starting point for evaluating the mechanistic underpinning of the craniofacial development in MS with the goal of linking specific clinical manifestations to molecular insights gained from hiPSC-based disease modeling