Understanding how people living with HIV/AIDS (PLWHA) experience social support in evangelical congregations

The HIV epidemic continues to be a global health crisis with an estimated 38 million people living with HIV worldwide. It is increasingly important to examine the psychosocial and spiritual issues that are present among people living with HIV/AIDS (PLWHA) to help decrease at-risk behavior and stigma associated with HIV. Faith can be an empowering social support system for PLWHA. Evangelical congregations have the potential to be HIV-comportment communities. This qualitative study utilizes hermeneutic phenomenology to analyze and interpret the lived experiences of 12 PLWHA who have a connection to an evangelical congregation. Anti-Oppression Theory and the intersectionality of faith and HIV, as it relates to oppression and empowerment, provide the conceptual framework for this study. Six major themes emerged from the data: Multifaceted Stigma, Power and Oppression, Patients as Educators, Our Voices Matter: What Works and What Doesn’t, Where Do We Fit In, and Mortality with Eternity in Mind. The most prominent theme, Multifaceted Stigma, identified congregational discrimination, lack of knowledge, and negative attitudes about HIV towards participants in this study. Findings highlighted a variety of psycho-social stressors exhibited by PLWHA, the importance of perceived and received support in congregations, and the intersection between spirituality and Anti-Oppressive Practice. The implications of these findings indicate the importance of PLWHA’s individual and collective voices; provide suggestions for evangelical congregations to become HIV-competent communities; and discuss the role of congregational social work with PLWHA

Successes, Challenges and Opportunities for Environmental Barrier Coatings

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Seismic Monitoring of Permafrost in Svalbard, Arctic Norway

We analyze data from passive and active seismic experiments conducted in the Adventdalen valley of Svalbard in the Norwegian Arctic. Our objective is to characterize the ambient wavefield of the region and to investigate permafrost dynamics through estimates of seismic velocity variations. We are motivated by a need for early geophysical detection of potentially hazardous changes to permafrost stability. We draw upon several data sources to constrain various aspects of seismic wave propagation in Adventdalen. We use f-k analysis of five years of continuous data from the Spitsbergen seismic array (SPITS) to demonstrate that ambient seismic noise on Svalbard consists of continuously present body waves and intermittent surface waves appearing at regular intervals. A change in wavefield direction accompanies the sudden onset of surface waves when the average temperature rises above the freezing point, suggesting a cryogenic origin. This hypothesis is supported further by our analysis of records from a temporary broadband network, which indicates that the background wavefield is dominated by icequakes. Synthetic Green's functions calculated from a 3D velocity model match well with empirical Green's functions constructed from the recorded ambient seismic noise. We use a shallow shear-wave velocity model, obtained from active seismic measurements, to estimate the maximum depth of Rayleigh wave sensitivity to changes in shear velocity to be in the 50-100 m range. We extract seasonal variations in seismic velocities from ambient noise cross-correlation functions computed over three years of SPITS data. We attribute relative velocity variations to changes in the ice content of the shallow (2-4 m depth) permafrost, which is sensitive to seasonal temperature changes. A linear decreasing trend in seismic velocity is observed over the years, most likely due to permafrost warming.Peer reviewe

CMAS Reactive Coatings for TBCs

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Public responses to precautionary information from the department of health (UK) about possible health risks from mobile phones

Understanding public perceptions of health information is of increasing importance in the light of the growing imperatives upon regulators to communicate information about risk and uncertainty. Communicating the possible health risks from mobile telecommunications is a domain that allows consideration of both public perceptions of uncertain public health information and public responses to precautionary advice. This research reports the results of a nationally representative survey in the UK (n = 1742) that explored public responses to a leaflet issued by the Department of Health (DoH) in 2000 providing information about the possible health risks of mobile phones. The aims of the study were twofold: a) to assess awareness of the leaflet and the extent to which participants could identify the precautionary advice that the leaflet contained as coming from the Government; and b) to examine publics’ responses to the current Government precautionary advice about mobile phone health risks; was this associated with increased concern or reassurance? The results indicate the importance of policy makers developing a clear understanding of the possible effects of communicating precautionary advice.Mobile Telecommunications and Health Research Programm

West Nile virus methyltransferase domain interacts with protein kinase G

Background: The flaviviral nonstructural protein 5 (NS5) is a phosphoprotein, though the precise identities and roles of many specific phosphorylations remain unknown. Protein kinase G (PKG), a cGMP-dependent protein kinase, has previously been shown to phosphorylate dengue virus NS5. Methods: We used mass spectrometry to specifically identify NS5 phosphosites. Co-immunoprecipitation assays were used to study protein-protein interactions. Effects on viral replication were measured via replicon system and plaque assay titering. Results: We identified multiple sites in West Nile virus (WNV) NS5 that are phosphorylated during a WNV infection, and showed that the N-terminal methyltransferase domain of WNV NS5 can be specifically phosphorylated by PKG in vitro. Expressing PKG in cell culture led to an enhancement of WNV viral production. We hypothesized this effect on replication could be caused by factors beyond the specific phosphorylations of NS5. Here we show for the first time that PKG is also able to stably interact with a viral substrate, WNV NS5, in cell culture and in vitro. While the mosquito-borne WNV NS5 interacted with PKG, tick-borne Langat virus NS5 did not. The methyltransferase domain of NS5 is able to mediate the interaction between NS5 and PKG, and mutating positive residues in the αE region of the methyltransferase interrupts the interaction. These same mutations completely inhibited WNV replication. Conclusions: PKG is not required for WNV replication, but does make a stable interaction with NS5. While the consequence of the NS5:PKG interaction when it occurs is unclear, mutational data demonstrates that this interaction occurs in a region of NS5 that is otherwise necessary for replication. Overall, the results identify an interaction between virus and a cellular kinase and suggest a role for a host kinase in enhancing flaviviral replication

Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system.

Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the genomes of diverse organisms. These advances expand the potential of in vivo imaging experiments and facilitate experimentation with new, bright, photostable fluorescent proteins. Most quantitative comparisons of the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that govern their performance in cells or organisms. To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. We generated transgenic Caenorhabditis elegans strains expressing green, yellow, or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. We found that mNeonGreen was not as bright in vivo as predicted based on in vitro data but is a better tag than GFP for specific kinds of experiments, and we report on optimal red fluorescent proteins. These results identify ideal fluorescent proteins for imaging in vivo in C. elegans embryos and suggest good candidate fluorescent proteins to test in other animal model systems for in vivo imaging experiments

West Nile Virus Methyltransferase Domain Interacts with Protein Kinase

Background The flaviviral nonstructural protein 5 (NS5) is a phosphoprotein, though the precise identities and roles of many specific phosphorylations remain unknown. Protein kinase G (PKG), a cGMP-dependent protein kinase, has previously been shown to phosphorylate dengue virus NS5. Methods We used mass spectrometry to specifically identify NS5 phosphosites. Co-immunoprecipitation assays were used to study protein-protein interactions. Effects on viral replication were measured via replicon system and plaque assay titering. Results We identified multiple sites in West Nile virus (WNV) NS5 that are phosphorylated during a WNV infection, and showed that the N-terminal methyltransferase domain of WNV NS5 can be specifically phosphorylated by PKG in vitro. Expressing PKG in cell culture led to an enhancement of WNV viral production. We hypothesized this effect on replication could be caused by factors beyond the specific phosphorylations of NS5. Here we show for the first time that PKG is also able to stably interact with a viral substrate, WNV NS5, in cell culture and in vitro. While the mosquito-borne WNV NS5 interacted with PKG, tick-borne Langat virus NS5 did not. The methyltransferase domain of NS5 is able to mediate the interaction between NS5 and PKG, and mutating positive residues in the αE region of the methyltransferase interrupts the interaction. These same mutations completely inhibited WNV replication. Conclusions PKG is not required for WNV replication, but does make a stable interaction with NS5. While the consequence of the NS5:PKG interaction when it occurs is unclear, mutational data demonstrates that this interaction occurs in a region of NS5 that is otherwise necessary for replication. Overall, the results identify an interaction between virus and a cellular kinase and suggest a role for a host kinase in enhancing flaviviral replication

Towards precision radial velocity science with SALT’s High-Resolution Spectrograph

We describe efforts to equip the Southern African Large Telescope (SALT) for precision radial velocity (PRV) work. Our current focus is on commissioning the high-stability (HS) mode of the High-Resolution Spectrograph (HRS), the mode intended to support exoplanet science. After replacing the original commercial iodine cell with a custom-built, precisely characterised one and following established best practice in terms of observing strategy and data reduction, this system now delivers 3-4 m/s radial velocity stability on 5th and 6th magnitude stars. Unfortunately, the throughput is compromised by the HRS dichroic split being at 555 nm (i.e. roughly midway through the 100 nm span of the iodine absorption spectrum). Furthermore, SALT’s fixed elevation axis limits the exposure time available for a given target and hence the depth and/or precision achievable with the iodine cell. The HS mode’s simultaneous ThAr option uses the full 370–890 nm passband of the HRS and does not suffer gas cell absorption losses, so it may be more suitable for exoplanet work. The first step was to quantify the internal stability of the spectrograph, which requires simultaneously injecting arc light into the object and calibration fibres. The HS mode’s optical feed was modified accordingly, stability test runs were conducted and the necessary analysis tools were developed. The initial stability test yielded encouraging results and though more testing is still to be done, SAL a laser frequency comb to support the development of HRS PRV capability

PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility

Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity upon mechanical deformation. For the first time, this study reports on the comparative analysis of PVDF and P(VDF-TrFE) electrospun scaffolds in terms of structural and piezoelectric properties as well as their in vitro performance. A dynamic impact test machine was developed, validated, and utilised, to evaluate the generation of an electrical voltage upon the application of an impact load (varying load magnitude and frequency) onto the electrospun PVDF (15–20 wt%) and P(VDF-TrFE) (10–20 wt%) scaffolds. The cytotoxicity and in vitro performance of the scaffolds was evaluated with neonatal rat (nrSCs) and adult human Schwann cells (ahSCs). The neurite outgrowth behaviour from sensory rat dorsal root ganglion neurons cultured on the scaffolds was analysed qualitatively. The results showed (i) a significant increase of the β-phase content in the PVDF after electrospinning as well as a zeta potential similar to P(VDF-TrFE), (ii) a non-constant behaviour of the longitudinal piezoelectric strain constant d33, depending on the load and the load frequency, and (iii) biocompatibility with cultured Schwann cells and guiding properties for sensory neurite outgrowth. In summary, the electrospun PVDF-based scaffolds, representing piezoelectric activity, can be considered as promising materials for the development of artificial nerve conduits for the peripheral nerve injury repair