Effects of surface reflectance and 3D shape on perceived rotation axis

Cataloged from PDF version of article.Surface specularity distorts the optic flow generated by a moving object in a way that provides important cues for identifying surface material properties (Doerschner, Fleming et al., 2011). Here we show that specular flow can also affect the perceived rotation axis of objects. In three experiments, we investigate how threedimensional shape and surface material interact to affect the perceived rotation axis of unfamiliar irregularly shaped and isotropic objects. We analyze observers' patterns of errors in a rotation axis estimation task under four surface material conditions: shiny, matte textured, matte untextured, and silhouette. In addition to the expected large perceptual errors in the silhouette condition, we find that the patterns of errors for the other three material conditions differ from each other and across shape category, yielding the largest differences in error magnitude between shiny and matte, textured isotropic objects. Rotation axis estimation is a crucial implicit computational step to perceive structure from motion; therefore, we test whether a structure from a motion-based model can predict the perceived rotation axis for shiny and matte, textured objects. Our model's predictions closely follow observers' data, even yielding the same reflectance-specific perceptual errors. Unlike previous work (Caudek & Domini, 1998), our model does not rely on the assumption of affine image transformations; however, a limitation of our approach is its reliance on projected correspondence, thus having difficulty in accounting for the perceived rotation axis of smooth shaded objects and silhouettes. In general, our findings are in line with earlier research that demonstrated that shape from motion can be extracted based on several different types of optical deformation (Koenderink & Van Doorn, 1976; Norman & Todd, 1994; Norman, Todd, & Orban, 2004; Pollick, Nishida, Koike, & Kawato, 1994; Todd, 1985). © 2013 Arvo

Effects of surface reflectance and 3D shape on perceived rotation axis

Surface specularity distorts the optic flow generated by a moving object in a way that provides important cues for identifying surface material properties (Doerschner, Fleming et al., 2011). Here we show that specular flow can also affect the perceived rotation axis of objects. In three experiments, we investigate how threedimensional shape and surface material interact to affect the perceived rotation axis of unfamiliar irregularly shaped and isotropic objects. We analyze observers' patterns of errors in a rotation axis estimation task under four surface material conditions: shiny, matte textured, matte untextured, and silhouette. In addition to the expected large perceptual errors in the silhouette condition, we find that the patterns of errors for the other three material conditions differ from each other and across shape category, yielding the largest differences in error magnitude between shiny and matte, textured isotropic objects. Rotation axis estimation is a crucial implicit computational step to perceive structure from motion; therefore, we test whether a structure from a motion-based model can predict the perceived rotation axis for shiny and matte, textured objects. Our model's predictions closely follow observers' data, even yielding the same reflectance-specific perceptual errors. Unlike previous work (Caudek & Domini, 1998), our model does not rely on the assumption of affine image transformations; however, a limitation of our approach is its reliance on projected correspondence, thus having difficulty in accounting for the perceived rotation axis of smooth shaded objects and silhouettes. In general, our findings are in line with earlier research that demonstrated that shape from motion can be extracted based on several different types of optical deformation (Koenderink & Van Doorn, 1976; Norman & Todd, 1994; Norman, Todd, & Orban, 2004; Pollick, Nishida, Koike, & Kawato, 1994; Todd, 1985). © 2013 Arvo

Synthesis of 3-aroyl-4-aryl-1-isopropylamino-4-piperidinols and evaluation of the cytotoxicities of the compounds against human hepatoma and breast cancer cell lines

Some 4-piperidinol derivatives were synthesized and their cytotoxicity was tested against human hepatoma (Huh7) and breast cancer (T47D) cells. Aryl part was changed as phenyl in 2a, 4-methylphenyl in 2b, 4-methoxyphenyl in 2c, 4-chlorophenyl in 2d, 4-fluorophenyl in 2e, 4-bromophenyl in 2f, 4-nitrophenyl in 2g and 2-thienyl in 3. Compounds were synthesized and reported for the first time by this study except 2a and 2d. Chemical structures were confirmed by 1H NMR, 13C NMR, IR, MS and elemental analyses. Compounds 2a (3.1 times), 2c (3.8 times), 2f (4.6 times), 2g (1.3 times) and 3 (3.2 times) had 1.3-4.6 times higher cytotoxic potency than the reference compound 5-FU against Huh7 cell line while all the compounds synthesized had shown lower activities against T47D cell line than 5-FU. In the light of these results, compounds 2a, 2c, 2f, 2g and 3 may serve as model compounds for further studies. © 2014 Informa UK Ltd

Synthesis of new N,N′-bis[1-aryl-3-(piperidine-1-yl)propylidene] hydrazine dihydrochlorides and evaluation of their cytotoxicity against human hepatoma and breast cancer cells

N,N0-Bis[1-aryl-3-(piperidine-1-yl)propylidene]hydrazine dihydrochlorides were synthesized by the reaction of 2 mols of 1-aryl-3-(piperidine-1-yl)-1- propanone hydrochlorides with 1 mol of hydrazine hydrate. Aryl part was C 6H5 (P1), 4-CH3C6H4 (P2), 4-CH3OC6H4 (P3), 4-HOC6H 4 (P4), 4-ClC6H4 (P5), 3-CH3OC 6H4 (P6), 4-FC6H4 (P7) and 4-BrC6H4 (P8). Except P1, all compounds were reported for the first time. The chemical structures were confirmed by UV, 1H NMR, 13C NMR and HRMS spectra. P1, P2, P7 and P8 against human hepatoma (Huh7) cells and P1, P2, P4, P5, P6, P7 and P8 against breast cancer (T47D) cells have shown cytotoxicity. P1, P2 and P7 had more potent cytotoxicity against Huh7 cells than the reference compound 5-FU, whereas only P2 was more potent than the 5-FU against T47D cells. Representative compound P7 inhibited the mitochondrial respiration at 144, 264 and 424 mM concentrations dose-dependantly in liver homogenates. The results suggest that P1, P2, P7 and P8 may serve as model compounds for further synthetic studies. © 2014 Informa UK Ltd

Non-Coding Keratin Variants Associate with Liver Fibrosis Progression in Patients with Hemochromatosis

Background: Keratins 8 and 18 (K8/K18) are intermediate filament proteins that protect the liver from various forms of injury. Exonic K8/K18 variants associate with adverse outcome in acute liver failure and with liver fibrosis progression in patients with chronic hepatitis C infection or primary biliary cirrhosis. Given the association of K8/K18 variants with endstage liver disease and progression in several chronic liver disorders, we studied the importance of keratin variants in patients with hemochromatosis. Methods: The entire K8/K18 exonic regions were analyzed in 162 hemochromatosis patients carrying homozygous C282Y HFE (hemochromatosis gene) mutations. 234 liver-healthy subjects were used as controls. Exonic regions were PCRamplified and analyzed using denaturing high-performance liquid chromatography and DNA sequencing. Previouslygenerated transgenic mice overexpressing K8 G62C were studied for their susceptibility to iron overload. Susceptibility to iron toxicity of primary hepatocytes that express K8 wild-type and G62C was also assessed. Results: We identified amino-acid-altering keratin heterozygous variants in 10 of 162 hemochromatosis patients (6.2%) and non-coding heterozygous variants in 6 additional patients (3.7%). Two novel K8 variants (Q169E/R275W) were found. K8 R341H was the most common amino-acid altering variant (4 patients), and exclusively associated with an intronic KRT8 IVS7+10delC deletion. Intronic, but not amino-acid-altering variants associated with the development of liver fibrosis. I

Thrombosis in vasculitis: from pathogenesis to treatment

In recent years, the relationship between inflammation and thrombosis has been deeply investigated and it is now clear that immune and coagulation systems are functionally interconnected. Inflammation-induced thrombosis is by now considered a feature not only of autoimmune rheumatic diseases, but also of systemic vasculitides such as Behçet’s syndrome, ANCA-associated vasculitis or giant cells arteritis, especially during active disease. These findings have important consequences in terms of management and treatment. Indeed, Behçet’syndrome requires immunosuppressive agents for vascular involvement rather than anticoagulation or antiplatelet therapy, and it is conceivable that also in ANCA-associated vasculitis or large vessel-vasculitis an aggressive anti-inflammatory treatment during active disease could reduce the risk of thrombotic events in early stages. In this review we discuss thrombosis in vasculitides, especially in Behçet’s syndrome, ANCA-associated vasculitis and large-vessel vasculitis, and provide pathogenetic and clinical clues for the different specialists involved in the care of these patients

Childhood pemphigus vulgaris: five cases in 16 years

Pemphigus vulgaris (PV) usually occurs in adults. There are only a few reports of large PV series concerning childhood cases. We report here five cases of PV in patients younger than 16 years. They were analyzed among 169 PV cases out of a total of 192 pemphigus patients diagnosed between 1988-2004. The ratio of childhood cases was 2.9% in our large PV series. This relatively high ratio of childhood patients suggests that PV should not be neglected in the differential diagnosis of bullous lesions in childhood. Four of the five cases were followed up between 2-4 years and all of these four cases showed at least one relapse. PV also seems to show a relapsing course in the pediatric age group like in adults

In silico molecular docking and ADME studies of 1,3,4-thiadiazole derivatives in relation to in vitro PON1 activity

Background: Paraoxonase 1 (PON1) is a paraoxonase, arylesterase and lactonase associated with protection of lipoproteins and cell membranes against oxidative modification. Objective: Based on antioxidative properties of PON1 and significance of 1,3,4-thiadiazoles in pharmaceutical chemistry, herein we aimed to evaluate the potentials of 1,3,4-thiadiazole derivatives as PON1 activators. Methods: 2-[[5-(2,4-Difluoro/dichlorophenylamino)-1,3,4-thiadiazol-2-yl]thio]acetophenone derivatives (1-18) were in vitro evaluated for their activator effects on PON1 which was purified using ammonium sulfate precipitation (60-80%) and DEAE-Sephadex anion exchange chromatography. Molecular docking studies were performed for the detection of affinities of all compounds to the active site of PON1. Moreover, Absorption, Distribution, Metabolism and Excretion (ADME) properties of all compounds were also in silico predicted. In silico molecular docking and ADME studies were carried out according to modules of Schrodinger’s Maestro molecular modeling package. Results: All compounds, particularly compounds 10, 13 and 17, were determined as promising PON1 activators and apart from compound 1, all of them were detected in the active site of PON1. Besides, ADME results indicated that all compounds were potential orally bioavailable drug-like molecules. Conclusion: PON1 activators, compounds 10, 13 and 17 stand out as potential drug candidates for further antioxidant studies and these compounds can be investigated for their therapeutic effects in many disorders such as atherosclerosis, diabetes mellitus, obesity, chronic liver inflammation and many more. © 2019 Bentham Science Publishers

Cytotoxicities of novel hydrazone compounds with pyrrolidine moiety: inhibition of mitochondrial respiration may be a possible mechanism of action for the cytotoxicity of new hydrazones

N,N’-Bis[1-aryl-3-pyrrolidine-1-yl)propylidene]hydrazine dihydrochlorides (R1–R7) were synthesized by the reaction of 2 mols of 1-aryl-3-(pyrrolidine-1-yl)-1-propanone hydrochlorides with 1 mol of hydrazine hydrate and reported for the first time with their detailed spectral analysis and cytotoxicities towards human hepatoma (Huh7) and breast cancer (T47D) cell lines. Compounds R2, R6, and R7 with the IC50 values of 5.16, 6.96, and 5.96 µM, respectively, showed higher cytotoxic potency than the reference compound 5-FU with 7.0 µM against Huh7 cell line. However, all compounds did not show better cytotoxic activities than 5-FU against T47D cell line at the conditions studied. The representative compound of series, R2, inhibited the mitochondrial respiration at 90, 165, and 265 µM concentrations in a dose dependent manner in liver homogenates, suggesting that mitochondrial respiration may be one of the contributing factor to the cytotoxicity of the compounds synthesized. The compounds R2, R6, and R7 can be chosen as the leader compounds of this study for further studies. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.Acknowledgements This study was supported by the Research Foundation of Atatürk University and the KANILTEK anticancer biomolecule screening facility, Bilkent University