RHAMM deficiency disrupts folliculogenesis resulting in female hypofertility

The postnatal mammalian ovary contains the primary follicles, each comprising an immature oocyte surrounded by a layer of somatic granulosa cells. Oocytes reach meiotic and developmental competence via folliculogenesis. During this process, the granulosa cells proliferate massively around the oocyte, form an extensive extracellular matrix (ECM) and differentiate into cumulus cells. As the ECM component hyaluronic acid (HA) is thought to form the backbone of the oocyte-granulosa cell complex, we deleted the relevant domain of the Receptor for HA Mediated Motility (RHAMM) gene in the mouse. This resulted in folliculogenesis defects and female hypofertility, although HA-induced signalling was not affected. We report that wild-type RHAMM localises at the mitotic spindle of granulosa cells, surrounding the oocyte. Deletion of the RHAMM C-terminus in vivo abolishes its spindle association, resulting in impaired spindle orientation in the dividing granulosa cells, folliculogenesis defects and subsequent female hypofertility. These data reveal the first identified physiological function for RHAMM, during oogenesis, and the importance of this spindle-associated function for female fertility

RHAMM deficiency disrupts folliculogenesis resulting in female hypofertility

The postnatal mammalian ovary contains the primary follicles, each comprising an immature oocyte surrounded by a layer of somatic granulosa cells. Oocytes reach meiotic and developmental competence via folliculogenesis. During this process, the granulosa cells proliferate massively around the oocyte, form an extensive extracellular matrix (ECM) and differentiate into cumulus cells. As the ECM component hyaluronic acid (HA) is thought to form the backbone of the oocyte-granulosa cell complex, we deleted the relevant domain of the Receptor for HA Mediated Motility (RHAMM) gene in the mouse. This resulted in folliculogenesis defects and female hypofertility, although HA-induced signalling was not affected. We report that wild-type RHAMM localises at the mitotic spindle of granulosa cells, surrounding the oocyte. Deletion of the RHAMM C-terminus in vivo abolishes its spindle association, resulting in impaired spindle orientation in the dividing granulosa cells, folliculogenesis defects and subsequent female hypofertility. These data reveal the first identified physiological function for RHAMM, during oogenesis, and the importance of this spindle-associated function for female fertility

An Architecture of Complexity

The Beln seorang arsitek Lucion kroitis yang dikenal secara internasional karena cara ikonoklastiknya dalam membuat arsitektur; bentuk coniplex dan idiosinkratiknya menyenangkan beberapa orang, membuat marah orang lain, dan menggelitik banyak orang. Bangunan fakultas kedokterannya untuk Universitas Louvain di luar Brusseis menimbulkan kontroversi luas pada awal 1970-an, penampilan mereka yang terfragmentasi dan improvisasi-hasil dari proses desain partisipatif yang disengaja-sangat kontras dengan rumah sakit besar dan berulang yang berdekatan, perwujudan dari birokrasi terpusat

Redéfinir le design «de la forme à l'habitabilité», par C. Thomas Mitchell

Kroll Lucien. Redéfinir le design «de la forme à l'habitabilité», par C. Thomas Mitchell. In: Les Annales de la recherche urbaine, N°57-58, 1992. Espaces publics en villes. pp. 223-224