26 research outputs found
Nutrigenomics and immune function in fish : new insights from omics technologies
This study was funded by BBSRC grant BB/M026604/1.Peer reviewedPublisher PD
Limits to sustained energy intake. XXIII. Does heat dissipation capacity limit the energy budget of lactating bank voles?
Acknowledgements We are grateful to our technicians and several students for their help during this study and for animal care. We thank Catherine Hambly and Peter Thompson for technical assistance for the isotope analysis for the DLW measurements. We thank Ulf Bauchinger for stimulating discussion and his comments, and two anonymous referees for comments on the manuscript. Funding This project was supported by grants from the Polish Ministry of Science and Higher Education [0595/B/P01/2011/40 to E.T.S. and 8167/B/P01/2011/40 to P.K.], and Jagiellonian University [DS/WBINOZ/INOS/757 to P.K.].Peer reviewedPublisher PD
Transcriptomic responses in the fish intestine
Acknowledgements This study was funded by BBSRC grant BB/M026604/1, and UK Technology Strategy Board (TSB) grant 11974-81166. CED was funded by a BBSRC EastBio PhD studentship at University of Aberdeen. We are grateful to Chris Secombes, Helen Dooley and the two anonymous referees who made valuable comments on the earlier version of the manuscript.Peer reviewedPublisher PD
Fur removal promotes an earlier expression of involution-related genes in mammary gland of lactating mice
Open Access via the Springer Compact Agreement We greatly benefited from discussing our work with Drs Torsten Stein, Wendy Woodward (who shared her raw data with us), and Sally Ward. We thank Catherine Hambly and Peter Thomson for assistance with isotope analyses. Acknowledgements We greatly benefited from discussing our work with Drs Torsten Stein, Wendy Woodward (who shared her raw data with us), and Sally Ward. We thank Catherine Hambly and Peter Thomson for assistance with isotope analyses. Funding The study was funded by Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/G009953/1, BB/P009875/1 and BB/J020029/1, awarded to JRS. EK was supported by BBSRC grants BB/C504794/1 and BB/R018812/1.Peer reviewedPublisher PD
Integration of Transcriptome, Gross Morphology and Histopathology in the Gill of Sea Farmed Atlantic Salmon (Salmo salar) : Lessons from Multi-site Sampling
Funding statement The study was supported by the Scottish Aquaculture Innovation Centre (SAIC grant SL 2017 08, ‘Nutritional Aspects of Gill Disease in Atlantic Salmon’) and co-funded by BioMar and Scottish Sea Farms (SSF). ACKNOWLEDGMENTS We thank SSF farm personnel for accommodating our research, performing gross morphology scoring and helping with sampling. Edinburgh Genomics is partly supported through core grants from NERC (R8/H10/56), MRC (MR/K001744/1), and BBSRC (BB/J004243/1). We also thank two reviewers for their comments on the earlier draft of the article. EK dedicates this paper to her late mother, Irena Król.Peer reviewedPublisher PD
Differential responses of the gut transcriptome to plant protein diets in farmed Atlantic salmon
Background The potential for alternative plant protein sources to replace limited marine ingredients in fish feeds is important for the future of the fish farming industry. However, plant ingredients in fish feeds contain antinutritional factors (ANFs) that can promote gut inflammation (enteritis) and compromise fish health. It is unknown whether enteritis induced by plant materials with notable differences in secondary metabolism is characterised by common or distinct gene expression patterns, and how using feeds with single vs mixed plant proteins may affect the gut transcriptome and fish performance. We used Atlantic salmon parr to investigate the transcriptome responses of distal gut to varying dietary levels (0-45%) of soy protein concentrate (SPC) and faba bean (Vicia faba) protein concentrate (BPC) following an 8-week feeding trial. Soybean meal (SBM) and fish meal (FM) were used as positive and negative controls for enteritis, respectively. Gene expression profiling was performed using a microarray platform developed and validated for Atlantic salmon. Results Different plant protein materials (SPC, BPC and SBM) generated substantially different gut gene expression profiles, with relatively few transcriptomic alterations (genes, pathways and GO terms) common for all plant proteins used. When SPC and BPC were simultaneously included in the diet, they induced less extensive alterations of gut transcriptome than diets with either SPC or BPC singly, probably due to reduced levels of individual ANFs. The mixed plant protein diets were also associated with improved body composition of fish relative to the single plant protein diets, which may provide evidence for a link between the magnitude of changes in gut transcriptome and whole-animal performance. Conclusions Our results indicate that gut transcriptomic profiling provides a useful tool for testing the applicability of alternative protein sources for aquaculture feeds and designing diets with reduced impact of ANFs on fish health. Ultimately, understanding diet-gut interactions and intestinal homeostasis in farmed fish is important to maximise performance and to ensure that aquaculture continues to be a sustainable source of food for a growing world population
Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences
Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine‐rich repeat receptor kinases (LRR‐RK) FLS2 and EFR, and the LRR receptor protein (LRR‐RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR‐RK and LRR‐RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP‐mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23‐regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2‐signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP‐type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity
Some Features of the Yamato-74013 Diogenite: The Opaque Minerals and the Abundances of Some Trace Elements
In a standard mineralogical-petrological microscopic examination of the Yamato-74013 diogenite attention was given to the opaque minerals, chromite and troilite. Iron-deficient sulphides are supposed to be responsible for some features observed on the thermomagnetic curves. Abundances of Sc, Co, Hg and Zn were determined with INAA and AA