Leading or Being Led: The Authentic Leadership Dilemma

This chapter explores how industrial PhD students are engaged in authenticleadership processes while coping with challenges through self-leadership.The authors illustrate how self-leadership can be a helpful approach tomanaging the leading-and-being-led dilemma. They argue thatself-leadership is a process of goal achievement in collaboration with keystakeholders and, therefore, an important aspect of authentic leadership. Theauthors identify four aspects of self-leadership that influence authenticity:roles, resources, relations and results. Kringelum, Mortensen and Holmgrencall for research into the emergence of self-leadership and authentic leadership,the leadership capabilities required and the double-sidedness anddilemmas inherent in such emergences across different contexts

High-throughput sequencing enhanced phage display enables the identification of patient-specific epitope motifs in serum

Phage display is a prominent screening technique with a multitude of applications including therapeutic antibody development and mapping of antigen epitopes. In this study, phages were selected based on their interaction with patient serum and exhaustively characterised by high-throughput sequencing. A bioinformatics approach was developed in order to identify peptide motifs of interest based on clustering and contrasting to control samples. Comparison of patient and control samples confirmed a major issue in phage display, namely the selection of unspecific peptides. The potential of the bioinformatic approach was demonstrated by identifying epitopes of a prominent peanut allergen, Ara h 1, in sera from patients with severe peanut allergy. The identified epitopes were confirmed by high-density peptide micro-arrays. The present study demonstrates that high-throughput sequencing can empower phage display by (i) enabling the analysis of complex biological samples, (ii) circumventing the traditional laborious picking and functional testing of individual phage clones and (iii) reducing the number of selection rounds.Fil: Christiansen, Anders. Technical University of Denmark; DinamarcaFil: Kringelum, Jens V.. Technical University of Denmark; DinamarcaFil: Hansen, Christian S.. Technical University of Denmark; DinamarcaFil: Bøgh, Katrine L.. Technical University of Denmark; DinamarcaFil: Sullivan, Eric. Roche Nimble Gen; Estados UnidosFil: Patel, Jigar. Roche Nimble Gen; Estados UnidosFil: Rigby, Neil M.. Institute of Food Research; Reino UnidoFil: Eiwegger, Thomas. Medical University of Vienna; AustriaFil: Szépfalusi, Zsolt. Medical University of Vienna; AustriaFil: Masi, Federico De. Technical University of Denmark; DinamarcaFil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Lund, Ole. Technical University of Denmark; DinamarcaFil: Dufva, Martin. Technical University of Denmark; Dinamarc

ChemProt-2.0: visual navigation in a disease chemical biology database

ChemProt-2.