Guest Artist Recital: George Krem, Trombone; Ludene Krem, Piano; October 16, 1972

Centennial East Recital HallMonday EveningOctober 16, 19728:15 p.m

Effect of Building Morphology on Energy and Structural Performance of High-Rise Office Buildings

The civil engineering and architectural communities are highly focused, these days, on designing buildings that maximize utilization of energy available from natural resources. This dissertation presents a quantitative study of the effect of high-rise office building morphology on energy and structural performances for the major climates. The parameters of the building morphologies are varied - the building footprint shape, the placement of the structural core/walls, and the building orientation. The energy analysis is performed using Autodesk Ecotect Analysis 2011; while using SAP2000 for the structure analysis and design. The key observations are: 1) the building morphology has a significant effect on the annual energy consumption, 2) placement of the structural core/walls in the east and west sides significantly improve the energy performance, 3) the tradeoff in the cost of placing the structural core/walls to maximize operating energy efficiency is too great, 4) for built to code buildings the energy demand may be considered marginally sensitive to changes in aspect ratio, and 5) high quality thermal properties of code-built envelope systems offer more flexibility to designers with regard to the building site planning without creating negative impacts on total energy demand

Bond and Flexural Behaviour of Self Consolidating Concrete Beams Reinforced and Prestressed with FRP Bars

Self consolidating concrete (SCC) is widely used in the construction industry. SCC is a high performance concrete with high workability and consistency allowing it to flow under its own weight without vibration and making the construction of heavily congested structural elements and narrow sections easier. Fiber reinforced polymer (FRP) reinforcement, with its excellent mechanical properties and non-corrosive characteristic, is being used as a replacement for conventional steel reinforcement. In spite of the wide spread of SCC applications, bond and flexural behaviour of SCC beams reinforced or prestressed with FRP bars has not been fully studied. Furthermore, the ACI 440.1R-06 equation for determining the development length of FRP bars is based on Glass FRP (GFRP) bars and may not be applicable for Carbon FRP (CFRP) bars. This research program included an experimental and analytical study to investigate the flexural and bond behaviour of SCC beams reinforced with FRP bars and SCC beams prestressed with CFRP bars. In the experimental phase, fifty-six beams were fabricated and tested. Sixteen of these beams were prestressed with CFRP bars and forty beams were reinforced with non-prestressed GFRP or CFRP bars. Four concrete batches were used to fabricate all the specimens. Three mixes were of self consolidating concrete (SCC) and one mix was of normal vibrated concrete (NVC). The test parameters for the non-prestressed beams were the concrete type, bar type and bar diameter, concrete cover thickness and embedment length while the test parameters for the prestressed beams were the concrete type and the prestressing level (30%, 45% and 60%). The transfer length of the prestressed CFRP bars was determined by means of longitudinal concrete strain profile and draw-in methods. All beams were tested in four-point bending to failure. Measurements of load, midspan deflection, bar slip if any at the beam ends, strain in reinforcing FRP bar at various locations, and strain in concrete at the beam midspan were collected during the flexural test. The concrete compressive strength at flexural tests of SCC mix-1, mix-2, and mix-3 were 62.1MPa, 49.6MPa and 70.9MPa, respectively and for the NVC mix was 64.5MPa. The material test results showed that SCC mixes had lower modulus of elasticity mechanical properties than the NVC mix. The modulus of elasticity of the SCC mixes ranged between 65% and 82% of the NVC mix. The modulus of rupture of the SCC mixes was 86% of the NVC mixes. The test results for beams prestressed with CFRP bars revealed that the variation of transfer length of CFRP bars in SCC versus their prestressing level was nonlinear. The average measured transfer lengths of 12.7mm diameter CFRP bars prestressed to 30%, 45% and 60% was found to be 25db, 40db, 54db, respectively. Measured transfer lengths of the 12.7mm diameter CFRP bar prestressed to 30% in SCC met the ACI440.4 prediction. However, as the prestressing level increased, the predicted transfer length became unconservative. At a 60% prestress level, the measured/prediction ratio was 1.25. Beams prestressed with CFRP bars and subjected to flexural testing with shear spans less than the minimum development length had local bar slippage within the transmission zone. Beams that experienced local bond slip, their stiffness was significantly decreased. A modification to the existing model used to calculate the transfer and development lengths of CFRP bars in NVC beams was proposed to account for the SCC. The test results for beams reinforced with FRP bars indicated that the average bond strength of CFRP bars in NVC concrete is about 15% higher than that of GFRP bars in NVC. The ACI 440.1R-06 equation overestimated the development length of the CFRP bars by about 40%, while CAN/CSA-S6-06 equation was unconservative by about 50%. A new factor of (1/1.35) was proposed to estimate the development length of the CFRP bars in NVC when the ACI440.1R-06 equation is used. Beams made from SCC showed closer flexural crack spacing than similar beams made from NVC at a similar loading. The deflection of beams made from SCC and reinforced with CFRP bars was found to be slightly larger than those made from NVC. The average bond stresses of GFRP and CFRP bars in SCC were comparable to those in NVC. However, FRP bars embedded in SCC beams had higher bond stresses within the uncracked region of the beams than those embedded in NVC beams. In contrast, FRP bars in SCC had lower bond stresses than FRP bars in NVC within the cracked region. The average bond strength of GFRP in SCC was increased by 15% when the concrete cover thickness increased from 1.0db to 3.0db. Cover thicknesses of 2db and 3db were found to be sufficient to prevent bond splitting failure of GFRP and CFRP bars in SCC, respectively. Bond splitting failure was recorded when the cover thickness dropped to 1.5db for the GRP bars and to 2.0db for the CFRP bars. An insignificant increase in average bond stress was found when the bar diameter decreased from 12.7mm to 6.3mm for the CFRP bars, and a similar increase occurred in GFRP bars when the bar diameter decreased from 15.9mm to 9.5mm. New models to calculate the development length of GFRP and CFRP bars embedded in SCC were proposed based on the experimental results. These models capture the average bond stress profile along the embedment length. A good agreement was found between the proposed model and the experimental results. Analytical modeling of the load-deflection response based on the effective moment of inertia (ISIS Canada M5) was unconservative for SCC beams reinforced with CFRP bars by 25% at ultimate loading. A new model for bond stress versus Ma/Mcr (applied moment to cracking moment) ratio was developed for GFRP and CFRP bars in SCC and for CFRP bars in NVC. These bond stress models were incorporated in a new rigorous model to predict the load-deflection response based on the curvature approach. The FRP bar extension and bond stress models were used to calculate the load-deflection response. With these models 90% of the calculated deflections were found to be within ± 15% of the experimental measured results for SCC beams reinforced with FRP bars. Analytical modeling of the load-deflection for NVC and SCC beams prestressed with CFRP bars are proposed done. The moment resistance was calculated using Sectional Analysis approach. The deflection was calculated using simplified and detailed methods. The simplified method was based on the effective moment of inertia while the detailed method was based on effective moment of inertia and effective centroid. The experimental results correlated well with the detailed method at higher loads range. This study provided an understanding of the mechanism of bond and flexural behaviour of FRP reinforced and prestressed SCC beams. The information presented in this thesis is valuable for designers using FRP bars as flexural reinforcement and also for the development of design guidelines for SCC structures

Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani

Citation: Chandrasekaran, M., Chandrasekar, R., Sa, T., & Sathiyabama, M. (2014). Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani. Retrieved from http://krex.ksu.eduSerine proteases generally share a relatively high degree of sequence identity and play a major role in the diversity of biological processes. Here we focus on three-dimensional molecular architecture of serine proteases from Alernaria solani. The difference in flexibility of active binding pockets and electrostatic surface potential distribution of serine proteases in comparison with other fungal species is reported in this study. In this study we have purified a serine protease from the early blight pathogen, Alernaria solani. MALDI-TOF-MS/MS analysis revealed that protease produced by A. solani belongs to alkaline serine proteases. AsP is made up of 403 amino acid residues with molecular weight of 42.1kDa (Isoelectric point (pI)-6.51) and molecular formula C[subscript 1859]H[subscript 2930]N[subscript 516]O[subscript 595]S[subscript 4]. The follow-up research on the molecular structure prediction is used for assessing the quality of A. solani Protease (AsP). The AsP protein structure model was built based on its comparative homology with serine protease using the program, MODELER. AsP had 16 β-sheets and 10 α-helices, with Ser[superscript 350] (G347-G357), Asp[superscript 158] (D158-H169) and His[superscript 193] (H193-G203) in separate turn/coil structures. Biological metal binding region situated near the 6th-helix and His[superscript 193] residue is responsible for metal binding site. In addition, the calcium ion is coordinated by the carboxyl groups of Lys[superscript 84], Ile[superscript 85], Lys[superscript 86], Asp[superscript 87], Phe[superscript 88], Ala[superscript 89], Ala[superscript 90] (K84-A90) for first calcium (Ca[superscript 2+]) binding site and carbonyl oxygen atom of Lys[superscript 244], Gly[superscript 245], Arg[superscript 246], Thr[superscript 247], Lys[superscript 248], Lys[superscript 249], and Ala[superscript 250] (K244–A250), for second Ca[superscript 2+] binding site. Moreover, Ramachandran plot analysis of protein residues falling into most favored secondary structures were determined (83.3%). The predicted molecular 3D structural model was further verified using PROCHECK, ERRAT and VADAR servers to confirm the geometry and stereo-chemical parameters of the molecular structural design. The functional analysis of AsP 3D molecular structure predictions familiar in the current study may provide a new perspective in the understanding and identification of antifungal protease inhibitor designing

Serine Proteases-Like Genes in the Asian Rice Gall Midge Show Differential Expression in Compatible and Incompatible Interactions with Rice

The Asian rice gall midge, Orseolia oryzae (Wood-Mason), is a serious pest of rice. Investigations into the gall midge-rice interaction will unveil the underlying molecular mechanisms which, in turn, can be used as a tool to assist in developing suitable integrated pest management strategies. The insect gut is known to be involved in various physiological and biological processes including digestion, detoxification and interaction with the host. We have cloned and identified two genes, OoprotI and OoprotII, homologous to serine proteases with the conserved His87, Asp136 and Ser241 residues. OoProtI shared 52.26% identity with mosquito-type trypsin from Hessian fly whereas OoProtII showed 52.49% identity to complement component activated C1s from the Hessian fly. Quantitative real time PCR analysis revealed that both the genes were significantly upregulated in larvae feeding on resistant cultivar than in those feeding on susceptible cultivar. These results provide an opportunity to understand the gut physiology of the insect under compatible or incompatible interactions with the host. Phylogenetic analysis grouped these genes in the clade containing proteases of phytophagous insects away from hematophagous insects

Правове регулювання безоплатної приватизації земель запасу

Досліджуються правові підстави безоплатної приватизації земель запасу. Автор виокремлює два її різновиди – гарантовану і негарантовану. Розкриваються особли­вості багатоетапної процедури реалізації права на безоплатну приватизацію земель запасу в Україні.Исследуются правовые основания бесплатной приватизации земель запаса. Автор разделяет два ее разновидности – гарантированную и негарантированную. Раскрыва­ются особенности многоэтапной процедуры реализации права на бесплатную прива­тизацию земель в Украине.Legal rules about free of payment land privatization are investigated. The author pro­poses dividing free of payment land privatization into two varieties: guaranteed and non­guaranteed ones. Legal peculiarities of multistoried procedure of free of payment land priva­tization in Ukraine are disclosed

A Drosophila Pattern Recognition Receptor Contains a Peptidoglycan Docking Groove and Unusual L,D-Carboxypeptidase Activity

The Drosophila peptidoglycan recognition protein SA (PGRP-SA) is critically involved in sensing bacterial infection and activating the Toll signaling pathway, which induces the expression of specific antimicrobial peptide genes. We have determined the crystal structure of PGRP-SA to 2.2-Å resolution and analyzed its peptidoglycan (PG) recognition and signaling activities. We found an extended surface groove in the structure of PGRP-SA, lined with residues that are highly diverse among different PGRPs. Mutational analysis identified it as a PG docking groove required for Toll signaling and showed that residue Ser158 is essential for both PG binding and Toll activation. Contrary to the general belief that PGRP-SA has lost enzyme function and serves primarily for PG sensing, we found that it possesses an intrinsic L,D-carboxypeptidase activity for diaminopimelic acid-type tetrapeptide PG fragments but not lysine-type PG fragments, and that Ser158 and His42 may participate in the hydrolytic activity. As L,D-configured peptide bonds exist only in prokaryotes, this work reveals a rare enzymatic activity in a eukaryotic protein known for sensing bacteria and provides a possible explanation of how PGRP-SA mediates Toll activation specifically in response to lysine-type PG

Defining genes: a computational framework

The precise elucidation of the gene concept has become the subject of intense discussion in light of results from several, large high-throughput surveys of transcriptomes and proteomes. In previous work, we proposed an approach for constructing gene concepts that combines genomic heritability with elements of function. Here, we introduce a definition of the gene within a computational framework of cellular interactions. The definition seeks to satisfy the practical requirements imposed by annotation, capture logical aspects of regulation, and encompass the evolutionary property of homology

Antifibrinolytic Role of a Bee Venom Serine Protease Inhibitor That Acts as a Plasmin Inhibitor

Bee venom is a rich source of pharmacologically active substances. In this study, we identified a bumblebee (Bombus ignitus) venom Kunitz-type serine protease inhibitor (Bi-KTI) that acts as a plasmin inhibitor. Bi-KTI showed no detectable inhibitory effect on factor Xa, thrombin, or tissue plasminogen activator. In contrast, Bi-KTI strongly inhibited plasmin, indicating that it acts as an antifibrinolytic agent; however, this inhibitory ability was two-fold weaker than that of aprotinin. The fibrin(ogen)olytic activities of B. ignitus venom serine protease (Bi-VSP) and plasmin in the presence of Bi-KTI indicate that Bi-KTI targets plasmin more specifically than Bi-VSP. These findings demonstrate a novel mechanism by which bumblebee venom affects the hemostatic system through the antifibrinolytic activity of Bi-KTI and through Bi-VSP-mediated fibrin(ogen)olytic activities, raising interest in Bi-KTI and Bi-VSP as potential clinical agents