Aneuploidy and Confined Chromosomal Mosaicism in the Developing Human Brain

BACKGROUND: Understanding the mechanisms underlying generation of neuronal variability and complexity remains the central challenge for neuroscience. Structural variation in the neuronal genome is likely to be one important mechanism for neuronal diversity and brain diseases. Large-scale genomic variations due to loss or gain of whole chromosomes (aneuploidy) have been described in cells of the normal and diseased human brain, which are generated from neural stem cells during intrauterine period of life. However, the incidence of aneuploidy in the developing human brain and its impact on the brain development and function are obscure. METHODOLOGY/PRINCIPAL FINDINGS: To address genomic variation during development we surveyed aneuploidy/polyploidy in the human fetal tissues by advanced molecular-cytogenetic techniques at the single-cell level. Here we show that the human developing brain has mosaic nature, being composed of euploid and aneuploid neural cells. Studying over 600,000 neural cells, we have determined the average aneuploidy frequency as 1.25-1.45% per chromosome, with the overall percentage of aneuploidy tending to approach 30-35%. Furthermore, we found that mosaic aneuploidy can be exclusively confined to the brain. CONCLUSIONS/SIGNIFICANCE: Our data indicates aneuploidization to be an additional pathological mechanism for neuronal genome diversification. These findings highlight the involvement of aneuploidy in the human brain development and suggest an unexpected link between developmental chromosomal instability, intercellural/intertissular genome diversity and human brain diseases

Trematode Parthenitae Infection Rate and Distribution of Kola Bay Actic Molluscs of the Genus Littorina

The study was carried out in 2018 in the littoral zone of Kola Bay during the autumn period in the areas of Abram Mys Settlement, Mishukovo Settlement and Retinskaya Bay. Molluscs of the genus Littorina were collected from various substrates: stones, algae, metal construction and soil. In addition, samples were taken not from a particular substrate, but along the borders of the littoral zone to study species composition of molluscs and common level of infection rate in study areas. Our data showed that L. saxatilis infested by mature trematode parthenitae prefer being on the surface of algae, metal structure, stones at low tide instead of hiding under the stones, in the thickness of algae and in other possible shelters. The study of age distribution among infested Littorina molluscs living on different substrates showed that the ratio of age groups is different and varies from 1+ to 13+. Distribution data are established and considered for infested molluscs by trematode parthenitae of the group ”pygmaeus”: Microphallus pygmaeus, M. piriformes, which are characterized by the absence of free-living cercariae. Trematode parthenitae of other species which have a stage of free-living cercaria in their life cycle are absent in molluscs during the autumn period

Spectral analysis of stationary components of periodically correlated random processes

The properties of cyclostationary process’ stationary components, selected using bandpass filtering, are considered. The estimates properties of their correlation and spectral characteristics are carried out. The last are built using Blackman–Tuki method

Properties of characteristics estimators of periodically correlated random processes in preliminary determination of the period of correlation

The coherent estimators of probabilistic characteristics of periodically correlated random processes with unknown period have been investigated. It is shown that these estimators are asymptotically unbiased and consistent. In a first approximation formulas were obtained for the bias and dispersion of estimators defining the impact of the preliminary determination of the period on the value of estimation error

Determining Patterns in the Stressed­deformed State of the Railroad Track Subgrade Reinforced with Tubular Drains

The technical condition of the railroad track subgrade has been analyzed, as well as the issues related to ensuring its strength and stability when exposed to floodwaters and when the track's sections are overmoistened during operation. As a result, it has been established that it is necessary to develop methods aimed at improving the subgrade's carrying capacity.The georadar research has explored the problematic areas of the railroad track subgrade, based on which the distribution of subgrade heterogeneity in the vertical plane, as well as the boundaries of its location, were established. Therefore, georadar research makes it possible to detect hidden defective sites in the subgrade without disrupting its strength characteristics.A technique has been proposed to improve the carrying capacity of the failed subgrade of a railroad track using the combined arrangement of drainage pipes in the vertical and horizontal directions in the railroad embankment. The special feature of this technique is the possibility to drain water at the different levels of surface water, which provides for an increase in the carrying capacity of the failed subgrade.The strained-deformed state of the subgrade reinforced with tubular drainage has been investigated. The result has proven the effectiveness of the use of tubular drainages to improve the carrying capacity of the railroad track overmoistened subgrade exposed to constant and temporary loads.This study findings have established that the deformity of the subgrade increases when using tubular drainage, though this occurs only in the initial period of its arrangement, in further operation, when it removes water from the subgrade body, the carrying capacity of the subgrade, on the contrary, will improve due to the enhanced physical and mechanical properties of soil

Procedure for Determining the Thermoelastic State of A Reinforced Concrete Bridge Beam Strengthened with Methyl Methacrylate

This paper reports the analysis of methods for determining temperature stresses and deformations in bridge structures under the influence of climatic temperature changes in the environment. A one-dimensional model has been applied to determine the temperature field and thermoelastic state in order to practically estimate the temperature fields and stresses of strengthened beams taking into consideration temperature changes in the environment. The temperature field distribution has been determined in the vertical direction of a reinforced concrete beam depending on the thickness of the structural reinforcement with methyl methacrylate. It was established that there is a change in the temperature gradient in a contact between the reinforced concrete beam and reinforcement. The distribution of temperature stresses in the vertical direction of a strengthened reinforced concrete beam has been defined, taking into consideration the thickness of the reinforcement with methyl methacrylate and the value of its elasticity module. It was established that the thickness of the reinforcement does not have a significant impact on increasing stresses while increasing the elasticity module of the structural reinforcement leads to an increase in temperature stresses. The difference in the derived stress values for a beam with methyl methacrylate reinforcement with a thickness of 10 mm and 20 mm, at elasticity module E=15,000 MPa, is up to 3 % at positive and negative temperatures. It has been found that there is a change in the nature of the distribution of temperature stresses across the height of the beam at the contact surface of the reinforced concrete beam and methyl methacrylate reinforcement. The value of temperature stresses in the beam with methyl methacrylate reinforcement and exposed to the positive and negative ambient temperatures increases by three times. It was established that the value of temperature stresses is affected by a difference in the temperature of the reinforced concrete beam and reinforcement, as well as the physical and mechanical parameters of the investigated structural materials of the beam and the structural reinforcement with methyl methacrylat

Devising A Procedure for Assessing the Subgrade Compaction Degree Based on the Propagation Rate of Elastic Waves

This paper reports the analysis of the methods for estimating the technical condition of the subgrade underneath a constructed railroad track or road during its operation. The study results have proven that the issue related to monitoring and controlling high-quality compaction of a heterogeneous subgrade remains relevant and requires the construction of reliable experimental methods for assessing the subgrade degree of compaction. A procedure for determining the compaction of subgrade in the laboratory has been devised, based on inertial microcomputer technologies, which makes it possible to assess the degree of compaction of subgrade soils depending on the propagation rate of an impact's elastic waves. An experimental study has been performed into the propagation rate of elastic waves across a homogeneous subgrade made of coarse-grained sand and a heterogeneous subgrade made of coarse sand with a layer of clay in the middle of the prism. The study results established that the propagation rate of an elastic wave in a heterogeneous subgrade accepts a lower value than the rate of wave propagation in a homogeneous subgrade. Through the dynamic interpretation, by using a discriminant statistical analysis, the characteristic features have been defined in the distribution of accelerations in the body of the homogeneous and heterogeneous subgrade, depending on the degree of compaction, which would make it possible to monitor the state of the subgrade during operation. As the degree of the subgrade soil compaction affects the technical condition of road

Devising A Procedure for Assessing the Subgrade Compaction Degree Based on the Propagation Rate of Elastic Waves

This paper reports the analysis of the methods for estimating the technical condition of the subgrade underneath a constructed railroad track or road during its operation. The study results have proven that the issue related to monitoring and controlling high-quality compaction of a heterogeneous subgrade remains relevant and requires the construction of reliable experimental methods for assessing the subgrade degree of compaction. A procedure for determining the compaction of subgrade in the laboratory has been devised, based on inertial microcomputer technologies, which makes it possible to assess the degree of compaction of subgrade soils depending on the propagation rate of an impact's elastic waves. An experimental study has been performed into the propagation rate of elastic waves across a homogeneous subgrade made of coarse-grained sand and a heterogeneous subgrade made of coarse sand with a layer of clay in the middle of the prism. The study results established that the propagation rate of an elastic wave in a heterogeneous subgrade accepts a lower value than the rate of wave propagation in a homogeneous subgrade. Through the dynamic interpretation, by using a discriminant statistical analysis, the characteristic features have been defined in the distribution of accelerations in the body of the homogeneous and heterogeneous subgrade, depending on the degree of compaction, which would make it possible to monitor the state of the subgrade during operation. As the degree of the subgrade soil compaction affects the technical condition of road