10 research outputs found
ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ ΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΡΡΠ±ΡΠΎΠ²ΡΡ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠΉ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΡΡ ΡΠΊΠ»Π°Π΄ΠΎΠΊ
The article is a brief review of publications devoted to the problem of persistent dysphonia. The main cause of voice disorders is the scarring of the vocal folds resulting from trauma, surgical manipulation, inflammatory process. Treatment of cicatricial lesions of the vocal folds remains a challenge, as far as existing methods do not ensure the recovery of the ultrastructure of the vocal folds. The authors present modern data on the structure of the vocal folds at the cellular level. Considered pathologic processes occur in different stages of scarring. Applied technologies of phonosurgery and conservative treatment, their effectiveness and shortcomings are covered. Analysis of experimental research conducted in the world demonstrates the promise of using the methods of tissue engineering to treat scarring of the vocal folds andΒ to restore the microstructure of the latter. Identified current issues remain unresolved, which leads to the need for further experimental and clinical studies in the treatment of this pathology.Π‘ΡΠ°ΡΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΡΠΎΠ±ΠΎΠΉ ΠΊΡΠ°ΡΠΊΠΈΠΉ ΠΎΠ±Π·ΠΎΡ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ ΠΌΠΈΡΠΎΠ²ΠΎΠΉ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ, ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΠΌ ΠΏΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΡΡΠ±ΡΠΎΠ²ΡΡ
ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠΉ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΡΡ
ΡΠΊΠ»Π°Π΄ΠΎΠΊ. ΠΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΡΡ
ΡΠΊΠ»Π°Π΄ΠΎΠΊ, Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡΠΈΠ΅ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΡΠ°Π²ΠΌ, Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΉ, Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, ΡΠ²Π»ΡΡΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΡΡΡ
ΠΏΡΠΈΡΠΈΠ½ ΡΡΠΎΠΉΠΊΠΎΠΉ Π΄ΠΈΡΡΠΎΠ½ΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΡΠ±ΡΠΎΠ²ΡΡ
ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠΉ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΡΡ
ΡΠΊΠ»Π°Π΄ΠΎΠΊ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎ-ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Ρ Π² ΡΠ²ΡΠ·ΠΈ Ρ ΡΠ΅ΠΌ, ΡΡΠΎ Π½Π΅ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΡΠ»ΡΡΡΠ°ΡΡΡΡΠΊΡΡΡΡ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΠΎΠΉ ΡΠΊΠ»Π°Π΄ΠΊΠΈ. ΠΠ²ΡΠΎΡΠ°ΠΌΠΈ ΠΈΠ·Π»ΠΎΠΆΠ΅Π½Ρ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΎ ΡΡΡΠΎΠ΅Π½ΠΈΠΈ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΡΡ
ΡΠΊΠ»Π°Π΄ΠΎΠΊ Π½Π° ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΌ ΡΡΠΎΠ²Π½Π΅. Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΡ, ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΡΡΠΈΠ΅ Π² ΡΠ°Π·Π½ΡΠ΅ ΡΡΠ°Π΄ΠΈΠΈ ΡΡΠ±ΡΠ΅Π²Π°Π½ΠΈΡ. ΠΡΠ²Π΅ΡΠ΅Π½Ρ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ΅ΠΌΡΠ΅ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠΎΠ½ΠΎΡ
ΠΈΡΡΡΠ³ΠΈΠΈ ΠΈ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ, ΠΈΡ
ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΊΠΈ. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΡΡ
Π² ΠΌΠΈΡΠ΅, Π΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΡΡΡΠΈΡ
Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ Π² Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠΈ ΠΌΠΈΠΊΡΠΎΡΡΡΡΠΊΡΡΡΡ Π³ΠΎΠ»ΠΎΡΠΎΠ²ΡΡ
ΡΠΊΠ»Π°Π΄ΠΎΠΊ, ΡΡΠΎ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΎ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΡΠΎΠΉΠΊΠΎΠΉ Π΄ΠΈΡΡΠΎΠ½ΠΈΠΈ. ΠΠ±ΠΎΠ·Π½Π°ΡΠ΅Π½Ρ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠ΅, ΠΎΡΡΠ°ΡΡΠΈΠ΅ΡΡ Π½Π΅ΡΠ΅ΡΠ΅Π½Π½ΡΠΌΠΈ Π²ΠΎΠΏΡΠΎΡΡ, ΡΡΠΎ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»ΠΈΠ²Π°Π΅Ρ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠΈΡ
ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π΄Π°Π½Π½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ
Calculation of fuel distribution in the combustion chamber front device equipped with a three-stage swirler
Bioengineered Bile Duct: the project resume and state of the art in 2018
Aim. Development of the physiologically relevant tissue-engineered graft for repair of bile duct injures (Dyuzheva et al., 2016). <br><br>Conclusions. Designed scaffold showed no cytotoxicity, both BM-MSCs and cholangiocytes migrated into the depth of fibrous material. The constructs was biodegradable in various model mediums: deionized water, phosphate buffer, bile, full culture medium. The next step is pre-clinical trials on rabbits and minipigs for assessment of implantation safety and efficacy. We suppose that this tubular multilayered tissue-engineered construct will be capable to integration in native tissues after implantation and may be used to injured bile duct reparation
Nonwoven polycaprolactone scaffolds for tissue engineering: The choice of the structure and the method of cell seeding
Nonwoven polycaprolactone materials produced by electrospinning are perspective internal prosthetic implants. Seeding these implants with multipotent mesenchymal stromal cells stimulates the replacement of the prosthesis with recipient's own connective tissue. Electrospinning method was used for producing polycaprolactone matrices differing in thickness, pore diameter, fiber size, and biomechanical properties. Labeled cells were seeded on scaffolds in three ways: (1) static, (2) dynamic, and (3) directed flow of the cell suspension generated by capillary action. Cell distribution on the surface and the interior of the scaffolds was studied; the metabolic activity of cells was measured by MTT assay. Static seeding method yielded fully confluence of cells covered the entire scaffold surface, but the cells were located primarily in the upper third of the matrix. Dynamic method proved to be effective only for scaffolds of thickness greater than 500 microns, irrespective of the pore diameter. The third method was effective only for scaffolds with the pore diameter of 20-30 microns, regardless of the material thickness. Resorbable nonwoven polycaprolactone electrospun materials have appropriate biomechanical properties and similar to native tissue matrix structures for internal prosthesis. The choice of the most effective cell seeding method depends on the spatial characteristics - the material thickness, pore diameter, and fibers size, which are determined by the electrospinning conditions
ΠΠ΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π° Π΄Π»Ρ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ: Π²ΡΠ±ΠΎΡ ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΡΠΏΠΎΡΠΎΠ±Π° Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ
Nonwoven polycaprolactone materials produced by electrospinning are perspective internal prosthetic implants. Seeding these implants with multipotent mesenchymal stromal cells stimulates the replacement of the prosthesis with recipient's own connective tissue. Electrospinning method was used for producing polycaprolactone matrices differing in thickness, pore diameter, fiber size, and biomechanical properties. Labeled cells were seeded on scaffolds in three ways: (1) static, (2) dynamic, and (3) directed flow of the cell suspension generated by capillary action. Cell distribution on the surface and the interior of the scaffolds was studied; the metabolic activity of cells was measured by MTT assay. Static seeding method yielded fully confluence of cells covered the entire scaffold surface, but the cells were located primarily in the upper third of the matrix. Dynamic method proved to be effective only for scaffolds of thickness greater than 500 microns, irrespective of the pore diameter. The third method was effective only for scaffolds with the pore diameter of 20-30 microns, regardless of the material thickness. Resorbable nonwoven polycaprolactone electrospun materials have appropriate biomechanical properties and similar to native tissue matrix structures for internal prosthesis. The choice of the most effective cell seeding method depends on the spatial characteristics - the material thickness, pore diameter, and fibers size, which are determined by the electrospinning conditions.ΠΠ΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ, ΡΠ²Π»ΡΡΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ°ΠΌΠΈ Π΄Π»Ρ ΡΠ½Π΄ΠΎΠΏΡΠΎΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΠΊΠΈΡ
ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΎΠ² ΠΌΡΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΠΌΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ Π·Π°ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅Π·Π° ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΎΠ΅Π΄ΠΈΠ½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΡΡ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΠ°. Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΠ»ΠΎΡΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ΅Ρ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π½Π΅ΡΠΊΠ°Π½ΡΡ
Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌΠΈ. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΡΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΠ° ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½ΠΎΠ²ΡΡ
ΠΌΠ°ΡΡΠΈΡ, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΡ
ΡΡ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ, Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡ ΠΈ Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ. ΠΠ°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΌΠ΅ΡΠ΅Π½ΡΠΌΠΈ ΠΌΡΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΠΌΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΏΡΠΏΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°ΡΠΈΠΊΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΡΠ΅ΠΌΡ ΡΠΏΠΎΡΠΎΠ±Π°ΠΌΠΈ: ΡΡΠ°ΡΠΈΡΠ½ΡΠΌ, Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ°. ΠΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΈ ΡΠΎΠ»ΡΠΈΠ½Π΅ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ², ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ·ΠΌΠ΅ΡΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΠ’Π’-ΡΠ΅ΡΡΠ°. Π‘ΡΠ°ΡΠΈΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ» ΠΏΠΎΠ»ΡΡΠΈΡΡ Π½ΠΎΡΠΈΡΠ΅Π»ΠΈ Ρ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΡΠΌ ΠΏΠΎΠΊΡΡΡΠΈΠ΅ΠΌ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΊΠ»Π΅ΡΠΊΠΈ Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌ ΡΠ°ΡΠΏΠΎΠ»Π°Π³Π°Π»ΠΈΡΡ Π² Π²Π΅ΡΡ
Π½Π΅ΠΉ ΡΡΠ΅ΡΠΈ ΠΌΠ°ΡΡΠΈΠΊΡΠ°. ΠΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΠΊΠ°Π·Π°Π»ΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ Π±ΠΎΠ»Π΅Π΅ 500 ΠΌΠΊΠΌ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΏΠΎΡ. ΠΠ΅ΡΠΎΠ΄ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ° Π±ΡΠ» ΡΡΡΠ΅ΠΊΡΠΈΠ²Π΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Ρ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡ 20-30 ΠΌΠΊΠΌ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ ΡΠΎΠ»ΡΠΈΠ½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°. ΠΠΈΠΎΡΠ΅Π·ΠΎΡΠ±ΠΈΡΡΠ΅ΠΌΡΠ΅ Π½Π΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎ-Π»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΡΡΠΈΠΌΠΈ Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ Π΄Π»Ρ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΡΡΠ΅Π½ΠΎΠΊ Π±ΡΡΡΠ½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΡΠΈ, ΠΈΠΌΠ΅ΡΡ ΡΡ
ΠΎΠ΄Π½ΠΎΠ΅ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠΌ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΡΡΠΎΠ΅Π½ΠΈΠ΅. ΠΡΠ±ΠΎΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ Π΅Π³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ - ΡΠΎΠ»ΡΠΈΠ½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°, Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΏΠΎΡ ΠΈ Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, ΠΊΠΎΡΠΎΡΡΠ΅, Π² ΡΠ²ΠΎΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΡΡ ΡΡΠ»ΠΎΠ²ΠΈΡΠΌΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°