Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases.

Main developmental programs are highly conserved among species of the animal kingdom. Improper execution of these programs often leads to progression of various diseases and disorders. Here we focused on Drosophila wing tissue morphogenesis, a fairly complex developmental program, one of the steps of which - apposition of the dorsal and ventral wing sheets during metamorphosis - is mediated by integrins. Disruption of this apposition leads to wing blistering which serves as an easily screenable phenotype for components regulating this process. By means of RNAi-silencing technique and the blister phenotype as readout, we identify numerous novel proteins potentially involved in wing sheet adhesion. Remarkably, our results reveal not only participants of the integrin-mediated machinery, but also components of other cellular processes, e.g. cell cycle, RNA splicing, and vesicular trafficking. With the use of bioinformatics tools, these data are assembled into a large blisterome network. Analysis of human orthologues of the Drosophila blisterome components shows that many disease-related genes may contribute to cell adhesion implementation, providing hints on possible mechanisms of these human pathologies

Дендрокліматологічні дослідження сосни звичайної в насадженні Лівобережного лісостепу України

Purpose of this research was to detect influence of climatic change on pine radial increment in stand in the Left-bank Forest-steppe of Ukraine as example 100-years-old stand at State Enterprise 'Kharkiv forest scientific research station’. Methods. Dendrochronological. Method of correlation function and multiple regressions (response function) were used to analyze the influence of climatic factors on tree radial increment. For this purpose computer program RESPO was used. Results. Tree ring series were built and were used for correlation analysis between climatic factors and pine radial increment for two periods: 1960-1988 and 1988-2016.Years of minimum (1933, 1942, 1956, 1972, 1976, 1976, 1987, 1992, 2002, 2006 та 2012) and maximum (1933, 1942, 1956, 1972, 1975, 1976, 1979, 1979, 1979, 1987, 1992, 2002 та 2012) of radial increment were detected.  Depressions of pine radial increment were as a result of negative weather conditions: low and high winter and early-spring temperature, droughts during vegetation period.  Maximums of radial increments were depended on favorable weather conditions for pine radial growth. Correlation and Response analysis for two periods showed that increase of temperature and precipitation, excluding winter precipitation, changed response of forest ecosystems to climatic change: if in first period (1960-1988) radial increment limited by April temperature and together July temperature positively influenced on radial growth, in second period (1988-2016) June temperature negatively infuenced on radial increment. Slight increase precipitation during vegetation period could not soften impact if high temperature during vegetatation period on tree radial increment. Conclusions. At comparison 1960-1988 and 1988-2016 it was detected that during first period positive influence of summer temperature on radial growth was distinctive and for second period one started to limit increment (negative influence) were detected. Slight increase of precipitation for vegetation period in second period significantly didn’t influence on pine radial growth. In second period increase of winter temperature and number of thaws negatively influenced on soil moisture, decreased of tree ring widths.Цель. Выявление влияния изменений климата на радиальный прирост сосны в Левобережной степи Украины на примере 100-летнего соснового насаждения ГП «Харьковская лесная научно-исследовательская станция». Методы. Дендрохронологические. Результаты. Созданы древесно-кольцевые хронологии для установления связей между климатическими показателями и радиальным приростом сосны за два периода: 1960-1988 та 1988-2016 гг. Корреляционный анализ и функция отклика за два периода показали, что при увеличении температур и количества осадков (исключением стали зимние осадки) изменилась реакция лесных екосистем на вариации условий природной среды. Так, если в первом периоде (1960-1988 гг.) радиальный прирост ограничивали апрельские температуры, в то же время на прирост положительно влияли летние (июльские) температуры, то во втором периоде летние температуры (июнькие) стали негативно влиять на прирост деревьев. Незначительное увеличение количества осадков за вегетационный период не смогло смягчить влияние высоких температур вегетационного периода на радиальный прирост. Выводы. При сравнении 1960-1988 и 1988-2016 гг. выявлено, что для первого периода характерно положительное влияние летних температур на радиальный прирост, однако для второго периода они начинают ограничивать прирост деревьев. Незначительное увеличение количества осадков за вегетационный период не смогло смягчить влияние высоких температур вегетационного периода на радиальный прирост.Мета. Виявлення впливу змін клімату на радіальний приріст сосни в Лівобережному степу на прикладі 100-річного соснового насадження ДП «Харківська лісова науково-дослідна станція». Методи. Дендрохронологічні. Результати. Створено деревно-кільцеві хронології, які використано у встановленні зв’язків між кліматичними чинниками та радіальним приростом сосни за два періоди: 1960-1988 та 1988-2016 рр. Кореляційний аналіз та функція відгуку за два періоди показали, що при збільшенні температур та кількості опадів (виняток – зимові опади) змінилася реакція лісових екосистем на зміни клімату: так, якщо в першому періоді (1960-1988 рр.) радіальний приріст обмежували квітневі температури, водночас позитивно впливали липневі температури, то у другому періоді (1988-2016 рр.) червневі температури вже негативно впливали на приріст. Незначне збільшення кількості опадів за вегетаційний період не змогли пом’якшити вплив високих температур вегетаційного періоду на радіальний приріст. Висновки. При порівнянні 1960-1988 та 1988-2016 рр. виявлено, що якщо для першого періоду характерний позитивний вплив літніх температур на радіальний приріст, то для другого періоду вони починають обмежувати приріст. Незначне збільшення кількості опадів за вегетаційний період у другому періоді не змогло пом’якшити вплив високих температур  на радіальний приріст дерев.

On the issue of the Black Sea methane hydrate potential development

First successful pilot test to produce methane from submarine gas hydrate field in the East Nankai trough offshore Japan has resumed practical interest to develop giant methane hydrate potential of the World Ocean and the Black Sea basin as well. The paper features geological aspects and technological problems of submarine gas hydrates exploitation and discuss methanehydrate potential assessment and promising exploration prospects in the Ukrainian part of the basin. The recommendations to methane hydrates development in the Black Sea are given

Distribution of ground beetles of the genus Amara Bonelli, 1810 (Coleoptera, Carabidae) in the agrolandscape in Northwestern Russia

The article investigates the abundance of beetles of the genus Amara in the agrolandscape of the Leningrad Region. In the period from 2004 to 2010, 14 Amara species were collected by pitfall trapping. Amara fulva (O.F. Müll.) is the most common species in the potato and clean fallow fields, Amara similata (Gyll.) – in the grain and grasses fields, and A. communis (Pz.) ˗ in adjacent semi-natural habitats respectively. The cluster analysis shows that assemblages of ground beetles of the genus Amara in fields of different crops, field boundaries, and adjacent habitats are separated from each other. In the agrolandscape, the population of beetles of the genus Amara reaches its greatest species richness and abundance in perennial grassfields. In addition, to our best knowledge, this research was a first time when Amara ingenua (Duft.) was observed feeding on the flowering spikelets of timothy grass (Phleum pratense L.)

Deep learning for inferring cause of data anomalies

Daily operation of a large-scale experiment is a resource consuming task, particularly from perspectives of routine data quality monitoring. Typically, data comes from different sub-detectors and the global quality of data depends on the combinatorial performance of each of them. In this paper, the problem of identifying channels in which anomalies occurred is considered. We introduce a generic deep learning model and prove that, under reasonable assumptions, the model learns to identify 'channels' which are affected by an anomaly. Such model could be used for data quality manager cross-check and assistance and identifying good channels in anomalous data samples. The main novelty of the method is that the model does not require ground truth labels for each channel, only global flag is used. This effectively distinguishes the model from classical classification methods. Being applied to CMS data collected in the year 2010, this approach proves its ability to decompose anomaly by separate channels.Comment: Presented at ACAT 2017 conference, Seattle, US

Nonequilibrium structural condition in the medical TiNi-based alloy surface layer treated by electron beam

The research is devoted to study the structural condition and their evolution from the surface to the depth of TiNi specimens treated by low-energy high-current electron beams with surface melting at a beam energy density E = 10 J/cm2, number of pulses N = 10, and pulse duration [tau] = 50 Ps. Determined thickness of the remelted layer, found that it has a layered structure in which each layer differs in phase composition and structural phase state. Refinement B2 phase lattice parameters in local areas showed the presence of strong inhomogeneous lattice strain

Kinetics of substrate recognition and cleavage by human 8-oxoguanine-DNA glycosylase

Human 8-oxoguanine-DNA glycosylase (hOgg1) excises 8-oxo-7,8-dihydroguanine (8-oxoG) from damaged DNA. We report a pre-steady-state kinetic analysis of hOgg1 mechanism using stopped-flow and enzyme fluorescence monitoring. The kinetic scheme for hOgg1 processing an 8-oxoG:C-containing substrate was found to include at least three fast equilibrium steps followed by two slow, irreversible steps and another equilibrium step. The second irreversible step was rate-limiting overall. By comparing data from Ogg1 intrinsic fluorescence traces and from accumulation of products of different types, the irreversible steps were attributed to two main chemical steps of the Ogg1-catalyzed reaction: cleavage of the N-glycosidic bond of the damaged nucleotide and β-elimination of its 3′-phosphate. The fast equilibrium steps were attributed to enzyme conformational changes during the recognition of 8-oxoG, and the final equilibrium, to binding of the reaction product by the enzyme. hOgg1 interacted with a substrate containing an aldehydic AP site very slowly, but the addition of 8-bromoguanine (8-BrG) greatly accelerated the reaction, which was best described by two initial equilibrium steps followed by one irreversible chemical step and a final product release equilibrium step. The irreversible step may correspond to β-elimination since it is the very step facilitated by 8-BrG

ОРГАНІЗАЦІЯ ВИХОВНОЇ РОБОТИ НА КАФЕДРІ ФАРМАКОЛОГІЇ З КЛІНІЧНОЮ ФАРМАКОЛОГІЄЮ

The article elucidates the educational work with students at the Pharmacology and Clinical Pharmacology Department of I. Ya. Horbachevsky Ternopil State Medical University. // o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "ojs.tdmu.edu.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;eУ статті висвітлено виховну роботу зi студентами Тернопільського державного медичного університету імені І. Я. Горбачевського на кафедрі фармакології з клінічною фармакологією.

Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides

The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 × 10−11 mol. dm−3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection