Non-destructive methods for mango ripening prediction: Visible and near-infrared spectroscopy (visNIRS) and laser Doppler vibrometry (LDV)

With up to 19% of mango fruit being lost during ripening, the need for non-destructive technologies to predict internal physiochemical traits is paramount. This study compared two non-destructive technologies, visible and near-infrared spectroscopy (visNIRS) and laser Doppler vibrometry (LDV), for predicting the ripeness of mango fruit in two cultivars, ‘Kent’ and ‘Keitt’. An internal quality index (IQI) in ‘Kent’ was predicted using visNIRS (RP2 = 0.729, RMSEP = 0.532) using partial least squares regression, which gave a single measure for ripeness incorporating firmness, sweetness, and pulp colour. This model was improved by using the sum of the individual sugar contents (glucose, sucrose, and fructose) over the conventional total soluble solids (TSS) measure. LDV provided poor predictions of firmness (R2 < 0.5) in both ‘Kent’ and ‘Keitt’ using least squares regression line. The resonant frequency, as measured by LDV, decreased linearly with time, while firmness quantified destructively (quasi-static) showed an exponential decrease, suggesting the vibrational and destructive firmness measure distinct characteristics, which would contribute to poor model performance. These results showed that LDV is not suitable for assessing mango ripening. While visNIRS models have been successful at predicting quality traits, our results suggested that using individual sugar content in place of TSS can improve the prediction of ripening. This understanding of the strengths and limitations of both visNIRS and LDV, and how they relate to destructive quality measurements, can be used to improve postharvest management practices whilst reducing commercial losses in the mango industry

Growing your own in times of crisis: the role of home food growing in perceived food insecurity and well-being during the early COVID-19 lockdown

Household food insecurity and poor well-being have increased during the coronavirus disease 2019 (COVID-19) pandemic and resulting lockdown measures. Home food growing has been associated with improved food access and well-being, but it is unknown what role it plays during food supply crises and lockdown. It is also unclear how home food growing and social restrictions may affect opinions about growing food in urban areas (i.e., urban agriculture [UA]). A cross-sectional online survey was conducted during the UK national lockdown in March-April 2020 to measure home food growing, perceived food insecurity, well-being, and opinions of UA. The participants were 477 UK-based adults (369 female, mean age 39.57 years ± 13.36); 152 participants were engaged in home food growing prior to the pandemic. Responses were compared to data collected from a separate sample of participants before the pandemic (N = 583) to explore potential shifts in opinions about UA. Participants who engaged in home food growing had lower levels of food insecurity (U ­= 19894.50, z = -3.649, p<.001, r = -.167) and higher well-being (U = 19566.50, z = -3.666, p<.001, r = -.168) than those not engaged in home food growing. Perceived food insecurity partially mediated the relationship between home food growing and well-being; home food growing was associated with less food insecurity, which in turn was associated with better well-being. There were no differences in opinions of UA compared to the sample of participants from before the pandemic. Home food growing may have had a protective effect over perceived food security and well-being in the early stages the pandemic. Opinions of UA were positive and unchanged compared to data collected pre-pandemic. Policies that support home food growing and access to suitable growing spaces and resources may be beneficial for food system resilience and well-being.Biotechnology and Biological Sciences Research Council (BBSRC): BB/S01425X/1, ESRC, NERC and the Scottish Governmen

Improving the tea withering process using ethylene or UV-C

Using a combination of biochemical, transcriptomic, and physiological analyses, we elucidated the mechanisms of physical and chemical withering of tea shoots subjected to UV-C and ethylene treatments. UV-C irradiation (15 kJ m–2) initiated oxidation of catechins into theaflavins, increasing theaflavin-3-monogallate and theaflavin digallate by 5- and 13.2–4.4-fold, respectively, at the end of withering. Concomitantly, a rapid change to brown/red, an increase in electrolyte leakage, and the upregulation of peroxidases (viz. Px2, Px4, and Px6) and polyphenol oxidases (PPO-1) occurred. Exogenous ethylene significantly increased the metabolic rate (40%) and moisture loss (30%) compared to control during simulated withering (12 h at 25 °C) and upregulated transcripts associated with responses to dehydration and abiotic stress, such as those in the ethylene signaling pathway (viz. EIN4-like, EIN3-FBox1, and ERFs). Incorporating ethylene during withering could shorten the tea manufacturing process, while UV-C could enhance the accumulation of flavor-related compounds

Potential of urban green spaces for supporting horticultural production: a national scale analysis

As urban areas and land-use constraints grow, there is increasing interest in utilizing urban spaces for food production. Several studies have uncovered significant potential for urban growing to supplement production of fruit and vegetables, focusing on one or two cities as case studies, whilst others have assessed the global scale potential. Here, we provide a national-scale analysis of the horticultural production potential of urban green spaces, which is a relevant scale for agri-food and urban development policy making using Great Britain (GB) as a case study. Urban green spaces available for horticultural production across GB are identified and potential yields quantified based on three production options. The distribution of urban green spaces within 26 urban towns and cities across GB are then examined to understand the productive potential compared to their total extent and populations. Urban green spaces in GB, at their upper limit, have the capacity to support production that is 8× greater than current domestic production of fruit and vegetables. This amounts to 38% of current domestic production and imports combined, or >400% if exotic fruits and vegetables less suited to GB growing conditions are excluded. Most urban green spaces nationally are found to fall within a small number of categories, with private residential gardens and amenity spaces making up the majority of space. By examining towns and cities across GB in further detail, we find that the area of green space does not vary greatly between urban conurbations of different sizes, and all are found to have substantial potential to meet the dietary needs of the local urban population. This study highlights that national policies can be suitably developed to support urban agriculture and that making use of urban green spaces for food production could help to enhance the resilience of the national-scale food system to shocks in import pathways, or disruptions to domestic production and distribution.Biotechnology and Biological Sciences Research Council (BBSRC): BB/S01425X/1, ESRC and NERC Scottish Governmen

Genetic control of stature in ornamental plants

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Analysis of developmental control genes using virus-induced gene silencing

A consistent challenge in studying the evolution of developmental processes has been the problem of explicitly assessing the function of developmental control genes in diverse species. In recent years, virus-induced gene silencing (VIGS) has proved to be remarkably adaptable and efficient in silencing developmental control genes in species across the angiosperms. Here we describe proven protocols for Nicotiana benthamiana and Papaver somniferum, representing a core and basal eudicot species.status: publishe

Is urban growing of fruit and vegetables associated with better diet quality and what mediates this relationship? Evidence from a cross-sectional survey.

Urban agriculture (UA), the growing of fruits and vegetables in urban and peri-urban areas, may improve food security and access, public health and dietary quality on both a broad and personal scale. However, there is little research on the relationship between UA and diet, and potential mediating factors are also unclear. This study aimed to investigate if proximity to and engagement with UA is associated with better diet quality, and what accounts for this relationship. UK-based adults (N=583, 69% Female) completed measures of proximity to and engagement with UA, perceived access to fruits and vegetables, health and ethical food choice motivations, connection with nature, psychological distress and dietary quality in an online survey. Participants were recruited from UA-related groups and the general public. Proposed relationships were analysed using a structural equation model. Greater proximity to and engagement with UA was associated with greater perceived access to fruits and vegetables, more health-related food choice motivations, more ethical-related food choice, feeling more connected with nature, and, surprisingly greater psychological distress. Furthermore, proximity to and engagement with UA was indirectly associated with better diet quality via health-, and ethical-related, food choice motivations. While the direct pathway between proximity to and engagement with UA and diet quality was not significant, UA is associated with better diet quality, partly via healthier and ethical food choice motivations. Upscaling UA may have benefits for dietary quality via these factors, and more research is needed to test causal relationships and understand these complex interactions

Urban agriculture in times of crisis: the role of home food growing in perceived food insecurity and well-being during the early COVID-19 lockdown

Household food insecurity and poor well-being have increased during the coronavirus disease 2019 (COVID-19) pandemic and resulting lockdown measures. Home food growing has been associated with improved food access and well-being, but it is unknown what role it plays during food supply crises and lockdown. It is also unclear how home food growing and social restrictions may affect opinions about growing food in urban areas (i.e., urban agriculture [UA]). A cross-sectional online survey was conducted during the UK national lockdown in March-April 2020 to measure home food growing, perceived food insecurity, well-being, and opinions of UA.  The participants were 477 UK-based adults (369 female, mean age 39.57 years ± 13.36); 152 participants were engaged in home food growing prior to the pandemic. Responses were compared to data collected from a separate sample of participants before the pandemic (N = 583) to explore potential shifts in opinions about UA. Participants who engaged in home food growing had lower levels of food insecurity (U ­= 19894.50, z = -3.649, p&lt;.001, r = -.167) and higher well-being (U = 19566.50, z = -3.666, p&lt;.001, r = -.168) than those not engaged in home food growing. Perceived food insecurity partially mediated the relationship between home food growing and well-being; home food growing was associated with less food insecurity, which in turn was associated with better well-being. There were no differences in opinions of UA compared to the sample of participants from before the pandemic. Home food growing may have had a protective effect over perceived food security and well-being in the early stages the pandemic. Opinions of UA were positive and unchanged compared to data collected pre-pandemic. Policies that support home food growing and access to suitable growing spaces and resources may be beneficial for food system resilience and well-being.</ns3:p

Scaling-up urban agriculture for a healthy, sustainable and resilient food system: the postharvest benefits, challenges and key research gaps

Sustainably ensuring food security and safety for the urban population is a major challenge. In this perspective, we present the concept of rurbanisation (the ruralisation of urban areas through increased urban agriculture) as a holistic strategy to provide a resilient food system. In particular, we focus on the postharvest benefits of urban agriculture for environmentally sustainable food supply chains, enhanced nutritional content of fresh produce and access to fresh, local and seasonal food. However, upscaling current urban agricultural systems requires improvement in current technologies and local infrastructure as well as the transfer of knowledge and skills to new urban farmers. This perspective summarises the main challenges that urban agriculture is currently facing from a postharvest quality and safety point of view, and highlights the research gaps and opportunities for improvements in that area

Bidirectional promoters in seed development and related hormone/stress responses

Background: Bidirectional promoters are common in genomes but under-studied experimentally, particularly in plants. We describe a targeted identification and selection of a subset of putative bidirectional promoters to identify genes involved in seed development and to investigate possible coordinated responses of gene pairs to conditions important in seed maturation such as desiccation and ABA-regulation. Results: We combined a search for 100–600 bp intergenic regions in the Arabidopsis genome with a cis-element based selection for those containing multiple copies of the G-box motif, CACGTG. One of the putative bidirectional promoters identified also contained a CE3 coupling element 5 bp downstream of one G-box and is identical to that characterized previously in the HVA1 promoter of barley. CE3 elements are significantly under-represented and under-studied in Arabidopsis. We further characterized the pair of genes associated with this promoter and uncovered roles for two small, previously uncharacterized, plant-specific proteins in Arabidopsis seed development and stress responses. Conclusions: Using bioinformatics we identified putative bidirectional promoters involved in seed development and analysed expression patterns for a pair of plant-specific genes in various tissues and in response to hormones/stress. We also present preliminary functional analysis of these genes that is suggestive of roles in seed development