Effect of weather, nitrogen fertilizer, and biostimulators on the root size and yield components of Hordeum vulgare

In this study, the effect of nitrogen doses (52, 80, 110, 140 kg/ha N) and the application of biostimulant preparations containing Ascophyllum nodosum L. algae extract were assessed. During the years 2018-2019, the influence of the preparations on the electrical capacity of the roots (C R) and yield components of spring barley was determined. Root electrical capacitance was determined in growth stages 45-50, 55-65, and 70-75 according to the BBCH-scale. The best phases of vegetation growth for the application of biostimulators with Ascophyllum nodosum extract were the barley tillering and elongation phases. This application increased C R while reducing the amount of N required to achieve similar or higher production of barley yield components compared to high N treatments. The root electrical capacitance, the number of productive tillers, and the number of grains per plant were significantly influenced (p > 0.05) by the weather of the year. The number of productive tillers was closely correlated with C R (r = 0.912∗∗) as well as the number of grains per plant (r = 0.859∗∗) and their weight (r = 0.850∗∗). These relationships were the highest at the beginning of the grain formation (BBCH 70-75). Foliar biostimulation was not very effective in the dry year of 2018. The problem may be the foliar application itself. The effect of foliar application is strongly dependent on weather conditions and may be ineffective in many cases. We recommend the foliar application of effective biostimulants in tillering and elongation phases. They can reduce production costs and environmental pollution by reducing the amount of fertilizer needed while maintaining yields.Internal Grant Agency of Mendel University in Brno [AF-IGA2019-IP053

Praxe studijního programu Učitelství pro mateřské školy: metodická příručka pro studenty

Title in English: Practice of the Study Program Teacher Training for Kindergartens: Methodical Handbook for Students The methodological manual was created with the aim of helping students of the bachelor's study program Teacher Training for Kindergartens to better understand the system of pedagogical and didactic practices so that they have a comprehensive idea of their organization and relationship to some theoretical subjects. At the same time, it should provide a deeper insight into the relationship between theory and practice through the portfolio and approach its function at the very end of the study, ie during the final state examination

The important figures of the French philology of Brno from the beginning up to now

This bachelor thesis deals with important persons of French philology of Brno from the beginning up to now and especially with one particular person - Otakar Novák. The work is divided into two parts. The first part describes the life and work of Otakar Novák before he started to work as a teacher at the university in Brno. The second part then describes his life and work since the beginning of his work in Brno until his death. Otakar Novák was born on 28 December 1905 in Vienna. He studied Czech and French language and literature at the university in Brno. Since 1946 he worked as an assistant at Charles University in Prague. In 1952 he was moved to Brno, where he worked until the end of his career. Besides being great teacher, he was lecturer at many conferences in Czechoslovakia and abroad, he has published seven books and dozens of articles, reports and reviews of French literature in particular and many forewords and afterwords to classics works of French literature. In 1969 he was elected chairman of the Circle of modern philologists and in 1964 was appointed professor of history of French literature. Otakar Novák was at the time the central figure of Romance studies of Brno and decisively contributed to its development in the area of French literary history, and helped her maintain an internationally comparable level

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

Actinobacteria belonging to the genus Rubrobacter are known for their multi-extremophilic growth conditions-they are highly radiation-resistant, halotolerant, thermotolerant or even thermophilic. This work demonstrates that the members of the genus are capable of accumulating polyhydroxyalkanoates (PHA) since PHA-related genes are widely distributed among Rubrobacter spp. whose complete genome sequences are available in public databases. Interestingly, all Rubrobacter strains possess both class I and class III synthases (PhaC). We have experimentally investigated the PHA accumulation in two thermophilic species, R. xylanophilus and R. spartanus. The PHA content in both strains reached up to 50% of the cell dry mass, both bacteria were able to accumulate PHA consisting of 3-hydroxybutyrate and 3-hydroxyvalerate monomeric units, none other monomers were incorporated into the polymer chain. The capability of PHA accumulation likely contributes to the multi-extremophilic characteristics since it is known that PHA substantially enhances the stress robustness of bacteria. Hence, PHA can be considered as extremolytes enabling adaptation to extreme conditions. Furthermore, due to the high PHA content in biomass, a wide range of utilizable substrates, Gram-stain positivity, and thermophilic features, the Rubrobacter species, in particular Rubrobacter xylanophilus, could be also interesting candidates for industrial production of PHA within the concept of Next-Generation Industrial Biotechnology

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due to their biodegradability and production from renewable sources. A potential increase in competitiveness of PHA production process comes with a combination of the use of thermophilic bacteria with the mutual use of waste substrates. In this work, the thermophilic bacterium Tepidimonas taiwanensis LMG 22826 was identified as a promising PHA producer. The ability to produce PHA in T. taiwanensis was studied both on genotype and phenotype levels. The gene encoding the Class I PHA synthase, a crucial enzyme in PHA synthesis, was detected both by genome database search and by PCR. The microbial culture of T. taiwanensis was capable of efficient utilization of glucose and fructose. When cultivated on glucose as the only carbon source at 50 & DEG;C, the PHA titers reached up to 3.55 g/L, and PHA content in cell dry mass was 65%. The preference of fructose and glucose opens the possibility to employ T. taiwanensis for PHA production on various food wastes rich in these abundant sugars. In this work, PHA production on grape pomace extracts was successfully tested.</p

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties