77 research outputs found

    Evidence of high genetic connectivity for the longnose spurdog Squalus blainville in the Mediterranean Sea

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    Squalus blainville is one of the least studied Mediterranean shark species. Despite being intensively fished in several locations, biological knowledge is limited and no genetic structure information is available. This is the first study to examine the genetic structure of S. blainville in the Mediterranean Sea. Considering the high dispersal potential inferred for other squalid sharks, the hypothesis of panmixia was tested based on a 585 bp fragment of the mitochondrial DNA cytochrome c oxidase subunit I gene from 107 individuals and six nuclear microsatellite loci from 577 individuals. Samples were collected across the Ionian, Aegean and Libyan Seas and off the Balearic Islands. Twenty three additional sequences of Mediterranean and South African origin were retrieved from GenBank and included in the mitochondrial DNA analysis. The overall haplotype diversity was high, in contrast to the low nucleotide diversity. Low and non-significant pairwise ΦST and FST values along with a Bayesian cluster analysis suggested high connectivity with subsequent genetic homogeneity among the populations studied, and thus a high dispersal potential for S. blainville similar to other squalids. The historical demography of the species was also assessed, revealing a pattern of population expansion since the middle Pleistocene. These findings could be considered in species-specific conservation plans, although sampling over a larger spatial scale and more genetic markers are required to fully elucidate the genetic structure and dispersal potential of S. blainville

    Strong population genetic structure and contrasting demographic histories for the small-spotted catshark (Scyliorhinus canicula) in the Mediterranean Sea

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    Coastal and demersal chondrichthyans, such as the small-spotted catshark, are expected to exhibit genetic differentiation in areas of complex geomorphology like the Mediterranean Basin because of their limited dispersal ability. To test this hypothesis, we used a fragment of the mitochondrial cytochrome c oxidase subunit I gene and 12 nuclear microsatellite loci in order to investigate the genetic structure and historical demography of this species, and to identify potential barriers to gene flow. Samples were collected from the Balearic Islands, the Algerian Basin, the Ionian Sea, the Corinthian Gulf and various locations across the Aegean Sea. Additional sequences from the Atlantic and the Levantine Basin retrieved from GenBank were included in the mitochondrial DNA analysis. Both mitochondrial and nuclear microsatellite DNA data revealed a strong genetic subdivision, mainly between the western and eastern Mediterranean, whereas the Levantine Basin shared haplotypes with both areas. The geographic isolation of the Mediterranean basins seems to enforce the population genetic differentiation of the species, with the deep sea acting as a strong barrier to its dispersal. Contrasting historical demographic patterns were also observed in different parts of the species' distribution, most notably a population growth trend in the western Mediterranean/ Atlantic area and a slight decreasing one in the Aegean Sea. The different effects of the Pleistocene glacial periods on the habitat availability may explain the contrasting demographic patterns observed. The current findings suggest that the smallspotted catshark exhibits several genetic stocks in the Mediterranean, although further study is needed

    Mobile Cloud Support for Semantic-Enriched Speech Recognition in Social Care

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    Nowadays, most users carry high computing power mobile devices where speech recognition is certainly one of the main technologies available in every modern smartphone, although battery draining and application performance (resource shortage) have a big impact on the experienced quality. Shifting applications and services to the cloud may help to improve mobile user satisfaction as demonstrated by several ongoing efforts in the mobile cloud area. However, the quality of speech recognition is still not sufficient in many complex cases to replace the common hand written text, especially when prompt reaction to short-term provisioning requests is required. To address the new scenario, this paper proposes a mobile cloud infrastructure to support the extraction of semantics information from speech recognition in the Social Care domain, where carers have to speak about their patients conditions in order to have reliable notes used afterward to plan the best support. We present not only an architecture proposal, but also a real prototype that we have deployed and thoroughly assessed with different queries, accents, and in presence of load peaks, in our experimental mobile cloud Platform as a Service (PaaS) testbed based on Cloud Foundry

    Autophagy: Regulation and role in disease

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    The development of phonological awareness throughout the school years: The case of a transparent orthography

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    This article discusses phonological awareness, reading decoding and spelling of 280 Greek elementary school students of all grades, with and without reading difficulties. Phonological awareness was assessed by using ten oral activities. Decoding was assessed for accuracy and fluency by using 80 real words and 20 non-words. Spelling was also assessed by using two different scoring methods. We explored differences in performance and developmental images between children with and without reading difficulties. The results revealed that the level of written language manipulation as well as of phonological awareness of children with reading difficulties was significantly lower than that of their peers without reading difficulties. This trend was recorded for all primary school grades. The differences were not eliminated despite the transition from grade to grade. Results pertaining to the development of phonological awareness are discussed in light of the transparent orthography of Greek language. © 2004 Kluwer Academic Publishers

    Phonological awareness skills: internal structure and hierarchy

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    The goal of this study was the examination of the internal structure of phonological awareness skills in the Greek language. A total number of 104 children participated in the study. All of them were first graders. Data collection took place during the first two weeks of the academic year 1997-8, in order to avoid the effects of reading instruction. The data collection instrument included thirteen exercises, of multiple items. A quantitative analysis of the data revealed that there are significant differences in the performance of children on the various levels of phonological awareness, especially in regard to syllable or phoneme awareness

    Strong population genetic structure and contrasting demographic histories for the small-spotted catshark (Scyliorhinus canicula) in the Mediterranean Sea

    No full text
    Coastal and demersal chondrichthyans, such as the small-spotted catshark, are expected to exhibit genetic differentiation in areas of complex geomorphology like the Mediterranean Basin because of their limited dispersal ability. To test this hypothesis, we used a fragment of the mitochondrial cytochrome c oxidase subunit I gene and 12 nuclear microsatellite loci in order to investigate the genetic structure and historical demography of this species, and to identify potential barriers to gene flow. Samples were collected from the Balearic Islands, the Algerian Basin, the Ionian Sea, the Corinthian Gulf and various locations across the Aegean Sea. Additional sequences from the Atlantic and the Levantine Basin retrieved from GenBank were included in the mitochondrial DNA analysis. Both mitochondrial and nuclear microsatellite DNA data revealed a strong genetic subdivision, mainly between the western and eastern Mediterranean, whereas the Levantine Basin shared haplotypes with both areas. The geographic isolation of the Mediterranean basins seems to enforce the population genetic differentiation of the species, with the deep sea acting as a strong barrier to its dispersal. Contrasting historical demographic patterns were also observed in different parts of the species' distribution, most notably a population growth trend in the western Mediterranean/Atlantic area and a slight decreasing one in the Aegean Sea. The different effects of the Pleistocene glacial periods on the habitat availability may explain the contrasting demographic patterns observed. The current findings suggest that the small-spotted catshark exhibits several genetic stocks in the Mediterranean, although further study is needed. © 2015 Macmillan Publishers Limited
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