91 research outputs found
Nutritional Deficiencies and Phospholipid Metabolism
Phospholipids are important components of the cell membranes of all living species. They contribute to the physicochemical properties of the membrane and thus influence the conformation and function of membrane-bound proteins, such as receptors, ion channels, and transporters and also influence cell function by serving as precursors for prostaglandins and other signaling molecules and modulating gene expression through the transcription activation. The components of the diet are determinant for cell functionality. In this review, the effects of macro and micronutrients deficiency on the quality, quantity and metabolism of different phospholipids and their distribution in cells of different organs is presented. Alterations in the amount of both saturated and polyunsaturated fatty acids, vitamins A, E and folate, and other micronutrients, such as zinc and magnesium, are discussed. In all cases we observe alterations in the pattern of phospholipids, the more affected ones being phosphatidylcholine, phosphatidylethanolamine and sphingomyelin. The deficiency of certain nutrients, such as essential fatty acids, fat-soluble vitamins and some metals may contribute to a variety of diseases that can be irreversible even after replacement with normal amount of the nutrients. Usually, the sequelae are more important when the deficiency is present at an early age
Lysosome-targeted octadecyl-rhodamine B-liposomes enhance lysosomal accumulation of glucocerebrosidase in Gaucher’s cells in vitro
Additive effect of red blood cell rigidity and adherence to endothelial cells in inducing vascular resistance
Increased apoptosis in cancer cells in vitro and in vivo by ceramides in transferrin-modified liposomes
Surface modification of liposomes with rhodamine-123-conjugated polymer results in enhanced mitochondrial targeting
Doxorubicin in TAT peptide-modified multifunctional immunoliposomes demonstrates increased activity against both drug-sensitive and drug-resistant ovarian cancer models
Targeted Transferrin-Modified Polymeric Micelles: Enhanced Efficacy in Vitro and in Vivo in Ovarian Carcinoma
In this study, transferrin (Tf)-modified
poly(ethylene glycol)-phosphatidylethanolamine
(mPEG-PE) micelles loaded with the poorly water-soluble drug, R547
(a potent and selective ATP-competitive cyclin-dependent kinase (CDK)
inhibitor), were prepared and evaluated for their targeting efficiency
and cytotoxicity in vitro and in vivo to A2780 ovarian carcinoma cells,
which overexpress transferrin receptors (TfR). At 10 mM lipid concentration,
both Tf-modified and plain micelles solubilized 800 μg of R547.
Tf-modified micelles showed enhanced interaction with A2780 ovarian
carcinoma cells in vitro. The involvement of TfR in endocytosis of
Tf-modified micelles was confirmed by colocalization studies of micelle-treated
cells with the endosomal marker Tf-Alexa488. We confirmed endocytosis
of micelles in an intact form with micelles loaded with a fluorescent
dye and additionally labeled with fluorescent lipid. The in vitro
cytotoxicity and in vivo tumor growth inhibition studies in A2780-tumor
bearing mice confirmed the enhanced efficacy of Tf-modified R547-loaded
micelles compared to free drug solution and to nonmodified micelles.
The results of this study demonstrate the potential application of
Tf-conjugated polymeric micelles in the treatment of tumors overexpressing
TfR
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