2,399 research outputs found
Digital Technologies in Youth Career Guidance Management for Professions of the Agricultural and Industrial Complex of the Sverdlovsk Region
The research is aimed at finding ways to effectively integrate traditional career guidance approaches and the capabilities of digital platforms working in the field of youth policy management in the Sverdlovsk region. The ways of intersection of interests of universities and youth policy management bodies in the emerging unified Internet space are outlined. The analysis of digital transformation process in the field of career guidance is carried out. The possibilities of the main federal information platforms in the field of youth work and their effectiveness in terms of indicators are presented: the number of events, the coverage of participants and, what is important for us- the project activities of young people with a career-oriented component. The article provides examples of social projects that already have a digital footprint and a sufficiently large involvement of schoolchildren and students of the Sverdlovsk region. The educational component containing elements of career guidance, which can be further strengthened, is of particular interest. The ways of creating a territorial multilevel digital model of youth career guidance of the Sverdlovsk region are outlined. Β© Published under licence by IOP Publishing Ltd.A major social project involving schoolchildren was carried out by the Ural SAU in partnership with the Sverdlovsk regional branch of the All-Russian Public Youth Organization "All-Russian Student Rescue Corps", with the support of the Autonomous Non-profit Organization "Resource Center for the Support of Volunteering in the Field of Safety Culture and Disaster Response Sverdlovsk region", and the Main Directorate of the Ministry of Emergency Situations of Russia in the Sverdlovsk region. The form of career guidance work through the joint participation of schoolchildren and students in such projects shows the social significance of joint actions, creates a community of like-minded people and helps to attract schoolchildren to the university
Current achievements in modifying crop genes using CRISPR/Cas system
With the advent of the new genome editing tool of target-specifically customizable endonucleases, a huge variety of novel opportunities have become feasible. The crop improvement is one of the main applications of genome editing in plant science and plant biotechnology. The amount of publications referring to genome editing and CRISPR/Cas system based molecular tools application in crops is permanently growing. The aim of this study is the systematization and cataloging of these data. Earlier we published the first catalog of targeted crop genome modifications as of February 10, 2017. The current review is an update of the catalog; it covers research papers on crop genome modifications from February 10, 2017 to August 17, 2018, found by searching 47 crop names in the Scopus database. Over one year and a half, 377 articles mentioning CRISPR/Cas and crop names have been published, of which 131 articles describe an experimental application of this tool for editing 193 genes in 19 crops, including rice with the largest number of genes modified (109 genes). Editing 50 of 193 genes was aimed at crop improvement. The catalog presented here includes these 50 genes, specifying the cultivars, each gene and gene product function, modification type and delivery method used. The current full list of genes modified with CRISPR/Cas with the aim of crop improvement is 81 in 16 crops (for 5 years from August 2013 to August 2018). In this paper, we also summarize data on different modifications types in different crops and provide a brief review of some novel methods and approaches that have appeared in crop genome editing research over the reviewed period. Taken together, these data provide a clear view on current progress in crop genome modifications and traits improvement using CRISPR/Cas based genome editing technology
Stimulating of entrepreneursβ innovative activity in the Republic of Bashkortostan
The article views some aspects of promoting innovative activity in the Republic of Bashkortostan. The measures stimulating innovative activity have been grouped into blocks.1. Creating favorable conditions for innovative activity. 2. Increasing the populationβs innovative activity. 3. Development of the system of scientific and production cooperation and innovation commercialization. 4. Investment support of innovative projects. 5. Development of innovative infrastructure. 6. Development of innovative small businesses. 7. Information provision of innovative activity. The main directions of implementing the above-mentioned conditions are: - lawmaking activity in the Republic, taking into consideration the federal legislation, international standards and traditions; - financial and tax inducement of scientific-technical and innovative activity; - international technological integration; - private-state partnership in the sphere of innovative activity. Recommendations are suggested, relating to the innovative policy activation for more efficient fulfillment of the key functions of the Republicβs scientific and research sector. The authors conclude that the Republic has all necessary preconditions for small entrepreneurship functioning: the natural conditions favorable for many kinds of economic activity, the forming local market infrastructure for small business servicing, and the availability of large enterprises and centers of economic activity.Keywords: entrepreneur, entrepreneurship, innovation, innovative activity, region, infrastructure, investments, stimulation, monitoring
ΠΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠ·ΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠ²ΡΡ ΠΏΡΠΎΡΠ΅Π°Π· ΠΏΠ»Π°Π·ΠΌΡ, ΠΌΠΎΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ ΠΈ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠΎΠ² ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ°
Aim. To study the level of activity of lysosomal cysteine proteases (cathepsins H, B, L) in blood plasma andΒ fractionated leukocytes (polymorphonuclear and mononuclear) in patients with Alzheimerβs disease in comparisonΒ with similar indicators in persons without signs of neurodegeneration as a possible marker of Alzheimerβs diseaseΒ development and diagnosis.Materials and methods. The spectrofluorimetric study of cathepsins B, L, H activity level in plasma andΒ fractionated leukocytes was conducted in 22 patients diagnosed with Alzheimerβs disease in comparison with theΒ same indicators in 22 patients matched by sex, age and associated diseases with patients of the observation group,Β but having no signs of neurodegeneration.Results. The activity of all three enzymes, and especially cathepsin H, increased significantly in blood plasma.Β A significant increase is also noted in the activity of cathepsins H, B, and L in homogenates of fractionatedΒ leukocytes. AtΒ the same time, in both polymorphonuclear and mononuclear leukocytes the greatest degree of changes is demonstratedΒ by the activity of cathepsin B, and the least is the activity of cathepsin L. Given the available data on an increasedΒ cathepsin B activity in the cerebrospinal fluid of patients with Alzheimerβs disease, we can assume a correlationΒ between the state of lysosomal proteases activity in the Central nervous system and in the peripheral blood cells.Conclusion. Alzheimerβs disease is associated with increased activity of cysteine cathepsins in plasma,Β polymorphonuclear and mononuclear leukocytes of peripheral blood, which can be considered as one of theΒ possible markers of development and diagnosis of the disease.Β Β Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠΈΡΡ ΡΡΠΎΠ²Π΅Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠ·ΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠ²ΡΡ
ΠΏΡΠΎΡΠ΅ΠΈΠ½Π°Π· (ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½ΠΎΠ² H, B, L) Π² ΠΏΠ»Π°Π·ΠΌΠ΅Β ΠΊΡΠΎΠ²ΠΈ ΠΈ ΡΡΠ°ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠ°Ρ
(ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
ΠΈ ΠΌΠΎΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
) ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π±ΠΎΠ»Π΅Π·Π½ΡΡΒ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ° Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ½ΡΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ Ρ Π»ΠΈΡ, Π½Π΅ ΠΈΠΌΠ΅ΡΡΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π½Π΅ΠΉΡΠΎΠ΄Π΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ,Β ΠΊΠ°ΠΊ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠΉ ΠΌΠ°ΡΠΊΠ΅Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½ΠΎΠ²Β B, L, H Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈ ΡΡΠ°ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠ°Ρ
22 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΠ°Π³Π½ΠΎΠ·ΠΎΠΌ Β«ΠΠΎΠ»Π΅Π·Π½Ρ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅Ρа» в ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ½ΡΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ 22 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΡΠΎΠΏΠΎΡΡΠ°Π²ΠΈΠΌΡΡ
ΠΏΠΎ ΠΏΠΎΠ»Ρ, Π²ΠΎΠ·ΡΠ°ΡΡΡ ΠΈ ΡΠΎΠΏΡΡΡΡΠ²ΡΡΡΠΈΠΌΒ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌΠΈ Π³ΡΡΠΏΠΏΡ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ, Π½ΠΎ Π½Π΅ ΠΈΠΌΠ΅ΡΡΠΈΡ
Β ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π½Π΅ΠΉΡΠΎΠ΄Π΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΡΠ΅Ρ
ΡΡΠ΅Ρ
ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²,Β Π² Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ β Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½Π° Π. Π Π³ΠΎΠΌΠΎΠ³Π΅Π½Π°ΡΠ°Ρ
ΡΡΠ°ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠΎΠ²Β ΡΠ°ΠΊΠΆΠ΅ ΠΎΡΠΌΠ΅ΡΠ°Π΅ΡΡΡ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½ΠΎΠ² Π, B, L, ΠΏΡΠΈ ΡΡΠΎΠΌ ΠΊΠ°ΠΊ Π²Β ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
, ΡΠ°ΠΊ ΠΈ Π² ΠΌΠΎΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠ°Ρ
Π² Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΈΠ·ΠΌΠ΅Π½ΡΠ΅ΡΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΒ ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½Π° Π, Π½Π°ΠΈΠΌΠ΅Π½ΡΡΠ΅ΠΉ βΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½Π° L. Π£ΡΠΈΡΡΠ²Π°Ρ ΠΈΠΌΠ΅ΡΡΠΈΠ΅ΡΡ Π΄Π°Π½Π½ΡΠ΅ ΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½Π°Β Π Π² ΡΠ΅ΡΠ΅Π±ΡΠΎΡΠΏΠΈΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π±ΠΎΠ»Π΅Π·Π½ΡΡ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ°, ΠΌΠΎΠΆΠ½ΠΎ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΡΒ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ΠΌ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΠ·ΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΠΈΠ½Π°Π· Π² ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π½Π΅ΡΠ²Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΈ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Β ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΊΡΠΎΠ²ΠΈ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»Π΅Π·Π½Ρ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ° Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π° Ρ Π½Π°ΡΠ°ΡΡΠ°Π½ΠΈΠ΅ΠΌ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠ²ΡΡ
ΠΊΠ°ΡΠ΅ΠΏΡΠΈΠ½ΠΎΠ² Π²Β ΠΏΠ»Π°Π·ΠΌΠ΅, ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
ΠΈ ΠΌΠΎΠ½ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠ°Ρ
ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ, ΡΡΠΎ ΠΌΠΎΠΆΠ΅Ρ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡΒ ΠΊΠ°ΠΊ ΠΎΠ΄ΠΈΠ½ ΠΈΠ· Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ
Spectroscopy of Helium Isotope 6He
The excited states of heavy helium isotope 6He were studied in stopped pion absorption in the reactions 9Be
Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin
Endolysins are peptidoglycan (PG) hydrolases that function as part of the bacteriophage (phage) lytic system to release progeny phage at the end of a replication cycle. Notably, endolysins alone can produce lysis without phage infection, which offers an attractive alternative to traditional antibiotics. Endolysins from phage that infect Gram-positive bacterial hosts contain at least one enzymatically active domain (EAD) responsible for hydrolysis of PG bonds and a cell wall binding domain (CBD) that binds a cell wall epitope, such as a surface carbohydrate, providing some degree of specificity for the endolysin. Whilst the EADs typically cluster into conserved mechanistic classes with well-defined active sites, relatively little is known about the nature of the CBDs and only a few binding epitopes for CBDs have been elucidated. The major cell wall components of many streptococci are the polysaccharides that contain the polyrhamnose (pRha) backbone modified with species-specific and serotype-specific glycosyl side chains. In this report, using molecular genetics, microscopy, flow cytometry and lytic activity assays, we demonstrate the interaction of PlyCB, the CBD subunit of the streptococcal PlyC endolysin, with the pRha backbone of the cell wall polysaccharides, Group A Carbohydrate (GAC) and serotype c-specific carbohydrate (SCC) expressed by the Group A Streptococcus and Streptococcus mutans, respectively
Cellulases from Mycelial fungi <em>Penicillium verruculosum</em> as a Real Alternative to Trichoderma Enzymes in Industrial Hydrolysis of Cellulosic Biomass
Abstract The possibility of using the recipient strain Penicillium verruculosum B1-537 (ΞniaD) as a producer of laboratory and industrial enzymes was considered. The advantage of this strain is its ability to secrete a basic cellulase complex consisting of cellobiohydrolases, endoglucanases, and Ξ²-glucosidase, which exceeds in its hydrolytic ability the enzyme complex of Hypocrea (Trichoderma) strains. Using the expression system, the basic complex of cellulases of the recipient strain Piptochaetium verruculosum B1-537 (ΞniaD) was supplemented with new (booster) enzymes that are necessary to increase its hydrolytic activity. Enzyme preparations adapted to the processing of various types of renewable plant biomass were obtained
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