489 research outputs found
Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
Aim: Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we investigated the effects of Ganoderma lucidum extract using SW 480 human colorectal cancer cell line. Materials and Methods: Two different fractions of Ganoderma lucidum extract, i.e., a fraction containing mainly polysaccharides (GLE-1), and a triterpenoid fraction without polysaccharides (GLE-2) were analyzed. Their antiproliferative activity was evaluated by cell proliferation assay and 3H-thymidine incorporation assay. Scavenging effects of DPPH radical were assessed using ESR-spectroscopy. Results: Our data showed that both GLE-1 and GLE-2 significantly inhibited the proliferation of SW 480 cells. The inhibitory effect of GLE-2 was much stronger than that of GLE-1. GLE-1 inhibited DNA synthesis in the cells and reduced the formation of DPPH radicals. Conclusion: Ganoderma lucidum extract inhibits proliferation of human colorectal cancer cells and possesses antioxidant properties.Π¦Π΅Π»Ρ: Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ, Π² ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΉ ΠΊΠΈΡΠ°ΠΉΡΠΊΠΎΠΉ
ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Π΅ ΡΠ°ΡΡΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Ganoderma lucidum. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ Π±ΡΠ»ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠΊΡΡΡΠ°ΠΊΡΠ° Ganoderma
lucidum Π½Π° ΠΊΠ»Π΅ΡΠΊΠΈ ΠΊΠΎΠ»ΠΎΡΠ΅ΠΊΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π»ΠΈΠ½ΠΈΠΈ SW 480. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΈΠ·ΡΡΠ°Π»ΠΈ Π΄Π²Π΅ ΡΡΠ°ΠΊΡΠΈΠΈ ΡΠΊΡΡΡΠ°ΠΊΡΠ°
Ganoderma lucidum: ΡΡΠ°ΠΊΡΠΈΡ GLE-1, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΡΡ ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄Ρ, ΠΈ ΡΡΠΈΡΠ΅ΡΠΏΠ΅Π½ΠΎΠΈΠ΄Π½ΡΡ ΡΡΠ°ΠΊΡΠΈΡ GLE-2. ΠΡ
ΡΠΎΡΡ-ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΡΡ
Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΈ ΠΏΠΎ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΡ 3
H-ΡΠΈΠΌΠΈΠ΄ΠΈΠ½Π°. ΠΠ»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ
Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΠ‘Π -ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΠΎΠ±Π΅ ΡΡΠ°ΠΊΡΠΈΠΈ Ganoderma lucidum ΠΏΠΎΠ΄Π°Π²Π»ΡΠ»ΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ
SW 480. Π ΠΎΡΡ-ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠΈΠΉ ΡΡΡΠ΅ΠΊΡ GLE-2 Π±ΡΠ» Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΌ, ΡΠ΅ΠΌ ΡΡΠ°ΠΊΡΠΈΠΈ GLE-1. Π€ΡΠ°ΠΊΡΠΈΡ GLE-1 ΠΏΠΎΠ΄Π°Π²Π»ΡΠ»Π° ΡΠΈΠ½ΡΠ΅Π· ΠΠΠ
Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Π»ΠΈΠ½ΠΈΠΈ SW 480 ΠΈ ΠΏΠΎΠ½ΠΈΠΆΠ°Π»Π° ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ DPPH ΡΠ°Π΄ΠΈΠΊΠ°Π»ΠΎΠ². ΠΡΠ²ΠΎΠ΄Ρ: ΡΠΊΡΡΡΠ°ΠΊΡ Ganoderma lucidum ΠΏΠΎΠ΄Π°Π²Π»ΡΠ΅Ρ
ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΊΠΎΠ»ΠΎΡΠ΅ΠΊΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΡΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ
Detection of SARS Coronavirus in Patients with Suspected SARS
Cases of severe acute respiratory syndrome (SARS) were investigated for SARS coronavirus (SARS-CoV) through RNA tests, serologic response, and viral culture. Of 537 specimens from patients in whom SARS was clinically diagnosed, 332 (60%) had SARS-CoV RNA in one or more clinical specimens, compared with 1 (0.3%) of 332 samples from controls. Of 417 patients with clinical SARS from whom paired serum samples were available, 92% had an antibody response. Rates of viral RNA positivity increased progressively and peaked at day 11 after onset of illness. Although viral RNA remained detectable in respiratory secretions and stool and urine specimens for >30 days in some patients, virus could not be cultured after week 3 of illness. Nasopharyngeal aspirates, throat swabs, or sputum samples were the most useful clinical specimens in the first 5 days of illness, but later in the illness viral RNA could be detected more readily in stool specimens
Environment-friendly surface acoustic wave humidity sensor with sodium alginate sensing layer
A low-cost and environment-friendly surface acoustic wave (SAW) humidity sensor was fabricated on a quartz substrate using sol-gel/spin-coated sodium alginate (SA) sensing layer. The sensing mechanism is based on the frequency shift of the SAW sensor caused by both mass loading and electrical loading, with the former being the dominant factor. The SA film prepared in this study is an environment-friendly material with a large number of hydroxyl and carboxylate groups, which easily adsorb and react with H2O molecules to form hydrogen bonds. These adsorbed H2O molecules lead to significantly enhanced mass loading and signal responses of the SAW sensor. Electrical loading effect is also generated due to the transfer of hydrogen ions in the H2O molecules, which alters the electrical resistance and results in changes of resonant frequencies of the SAW device. When the relative humidity (RH) is increased from 35% to 85%, the responses of the SAW sensor with 1β―wt% SA are significantly decreased. Whereas in a low humidity environment (e.g., RH <35%), the responses of the sensor show a linear relationship with the change of humidity. The developed humidity sensor shows good short-term/long-term stabilities and a low temperature coefficient of frequency
Facile Synthesis of ZnO Nanorods by Microwave Irradiation of ZincβHydrazine Hydrate Complex
ZnO nanorods have been successfully synthesized by a simple microwave-assisted solution phase approach. Hydrazine hydrate has been used as a mineralizer instead of sodium hydroxide. XRD and FESEM have been used to characterize the product. The FESEM images show that the diameter of the nanorods fall in the range of about 25β75 nm and length in the range of 500β1,500 nm with an aspect ratio of about 20β50. UVβVIS and photoluminescence spectra of the nanorods in solution have been taken to study their optical properties. A mechanism for microwave synthesis of the ZnO nanorods using hydrazine hydrate precursor has also been proposed
ZnO Nanorods via Spray Deposition of Solutions Containing Zinc Chloride and Thiocarbamide
In this work we present the results on formation of ZnO nanorods prepared by spray of aqueous solutions containing ZnCl2and thiocarbamide (tu) at different molar ratios. It has been observed that addition of thiocarbamide into the spray solution has great impact on the size, shape and phase composition of the ZnO crystals. Obtained layers were characterized by scanning electron microscopy (SEM) equipped with energy selected backscattered electron detection system (ESB), X-ray diffraction (XRD) and photoluminescence spectroscopy (PL). Small addition of thiocarbamide into ZnCl2solution (ZnCl2:tu = 1:0.25) supports development of significantly thinner ZnO nanorods with higher aspect ratio compared to those obtained from ZnCl2solution. Diameter of ZnO rods decreases from 270 to 100 nm and aspect ratio increases from βΌ2.5 to 12 spraying ZnCl2and ZnCl2:tu solutions, respectively. According to XRD, well crystallized (002) orientated pure wurtzite ZnO crystals have been formed. However, tiny βspotββlike formations of ZnS were detected on the side planes of hexagonal rods prepared from the thiocarbamide containing solutions. Being adsorbed on the side facets of the crystals ZnS inhibits width growth and promotes longitudinalc-axis growth
Trabectedin plus pegylated liposomal doxorubicin in relapsed ovarian cancer delays third-line chemotherapy and prolongs the platinum-free interval
Background: OVA-301 is a large randomized trial that showed superiority of trabectedin plus pegylated liposomal doxorubicin (PLD; CentoCor Ortho Biotech Products L.P., Raritan, NJ, USA). over single-agent PLD in 672 patients with relapsed ovarian cancer, particularly in the partially platinum-sensitive subgroup [platinum-free interval (PFI) of 6β12 months]. This superiority has been suggested to be due to the differential impact of subsequent (platinum) therapy
Measurements of the Mass and Full-Width of the Meson
In a sample of 58 million events collected with the BES II detector,
the process J/ is observed in five different decay
channels: , , (with ), (with
) and . From a combined fit of all five
channels, we determine the mass and full-width of to be
MeV/ and
MeV/.Comment: 9 pages, 2 figures and 4 table. Submitted to Phys. Lett.
A Measurement of Psi(2S) Resonance Parameters
Cross sections for e+e- to hadons, pi+pi- J/Psi, and mu+mu- have been
measured in the vicinity of the Psi(2S) resonance using the BESII detector
operated at the BEPC. The Psi(2S) total width; partial widths to hadrons,
pi+pi- J/Psi, muons; and corresponding branching fractions have been determined
to be Gamma(total)= (264+-27) keV; Gamma(hadron)= (258+-26) keV, Gamma(mu)=
(2.44+-0.21) keV, and Gamma(pi+pi- J/Psi)= (85+-8.7) keV; and Br(hadron)=
(97.79+-0.15)%, Br(pi+pi- J/Psi)= (32+-1.4)%, Br(mu)= (0.93+-0.08)%,
respectively.Comment: 8 pages, 6 figure
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