Concordancia entre la técnica de hemaglutinación indirecta e inmunoabsorción ligado a enzimas en el diagnóstico de toxoplasmosis porcina

El documento digital no refiere un asesorDetermina la concordancia entre las pruebas de HAI y ELISA, para detectar IgG anti-Toxoplasma gondii en el diagnóstico de toxoplasmosis porcina. El trabajo se desarrolla en 407 animales provenientes de crianzas porcinas ubicadas en la franja costera del departamento de Lima. Se colectan las muestras de sangre de cerdos en la fase de acabado, posteriormente las muestras de suero son conservadas en congelación (-70ºC) hasta su procesamiento en el Laboratorio de Parasitología de la Facultad de Medicina Veterinaria de la UNMSM. La concordancia de las técnicas diagnósticas y la seroprevalencia correspondiente se evalúan mediante dos diferentes modelos estadísticos: Indice de Kappa y la prueba de McNemar. En los resultados se halla que la concordancia entre las pruebas de HAI-ELISA a través del índice de Kapa es igual a 26% considerándose de tipo regular, con valores de 18.7 ± 3.8% por el método de HAI, y 14.7 ± 3.4%, por el método de ELISA; sin embargo, mediante la prueba de McNemar no se encuentra diferencias significativas y sugerirían que ambas técnicas son mutuamente reemplazables. Concluyendo que, como la correlación es regular, no se recomienda.Tesi

A GROUP OF CHALCOLITHIC POTTERY DISCOVERED AT ASOPOS TEPESI

Although the material culture of the Early and Late Chalcolithic periods are well defined and illustrated in Western Anatolia, the nature of the 5th millennium B.C. material culture is not clear, probably because of the limited number of excavations. The pottery assemblage unearthed in a thin cultural layer above the main rock in the Laodikeia/Asopos Tepesi belonging to the province of Denizli, provides valuable information on this barely known period. The forms and surface features of this pottery group reveal the existence of a settlement dated to the first half of the 5th millennium B.C. Though local qualities are dominant in the settlement, the traces of the interaction with the Aegean Islands are clearly visible

A GROUP OF CHALCOLITHIC POTTERY DISCOVERED AT ASOPOS TEPESI

Although the material culture of the Early and Late Chalcolithic periods are well defined and illustrated in Western Anatolia, the nature of the 5th millennium B.C. material culture is not clear, probably because of the limited number of excavations. The pottery assemblage unearthed in a thin cultural layer above the main rock in the Laodikeia/Asopos Tepesi belonging to the province of Denizli, provides valuable information on this barely known period. The forms and surface features of this pottery group reveal the existence of a settlement dated to the first half of the 5th millennium B.C. Though local qualities are dominant in the settlement, the traces of the interaction with the Aegean Islands are clearly visible

Public Health Reports ; v. 53, no. 24 : table of contents

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ARCHAEOLOGICAL AND ARCHAEOMETRIC OBSERVATIONS ON INHABITATION OF THE

Hoyuk Settlement is located within the boundary of Cal district of Denizli Province. It was strategically located on a natural passage and an important centre surrounded by walls during the 2nd millennium BC. Surveys conducted in the Upper Menderes Basin have revealed that the mountainous and plateau areas are at least as densely settled as in lowland sections and these areas have significant importance in archaeological point of view. An intensive archaeological survey was conducted in order to find answers on Hoyuk settlements. Therefore, The Middle and Late Bronze Age ceramics which were found in the settlement area were evaluated from archaeological and archaeometric perspective. These ceramics were characterized by using typological evaluations, optical microscopy and X-Ray Fluorescence analyses (XRF). Analogical evaluations shown that the settlement's interregional relations reach from Central Anatolian to the West Anatolian shores, but the local features known from Beycesultan are prominent. Archaeometric analyses revealed that the Middle and Late Bronze Age ceramics contain metamorphic rock fragments and very abundant carbonate. Small and fine quartz grains, a small amount of pyroxene and plagioclase minerals and abundant carbonate are observed in these samples. The results obtained from the intensive survey supported the idea that the importance of the settlements in the mountainous and plateau areas increased after the decline seen in the region in 16th century BC.C1 [Abay, Esref; Temur, Bora] Ege Univ, Fac Letters Protohist & Near Eastern Archaeol, Izmir, Turkey.[Konakci, Erim] Izmir Demokrasi Univ, Fac Sci & Letters Protohist & Near Eastern Archae, Izmir, Turkey.[Semiz, Baris] Pamukkale Univ, Dept Geol Engn, Denizli, Turkey

Naringenin induces tolerance to salt/osmotic stress through the regulation of nitrogen metabolism, cellular redox and ROS scavenging capacity in bean plants

The present study was conducted to uncover underlying possible effect mechanisms of flavonoid naringenin (Nar, 0.1–0.4 mM) in nitrogen assimilation, antioxidant response, redox status and the expression of NLP7 and DREB2A, on salt (100 mM NaCl) and osmotic-stressed (10% Polyethylene glycol, ?0.54 MPa) Phaseolus vulgaris cv. Yunus 90). Nar ameliorated salt/osmotic stresses-induced growth inhibition and improved the accumulation of proline, glycine betaine and choline. In response to stress, Nar increased endogenous content of nitrate (NO3?) and nitrite (NO2?) by regulating of nitrate reductase and nitrite reductase. Stress-triggered NH4+ was eliminated with Nar through increases in glutamine synthetase and glutamate synthase. After NaCl or NaCl + PEG exposure, Nar utilized the aminating activity of glutamate dehydrogenase in the conversion of NH4+. The stress-inducible expression levels of DREB2A were increased further by Nar, which might have affected stress tolerance of bean. Nar induced effectively the relative expression of NLP7 in the presence of the combination or alone of stress. Also, the impaired redox state by stress was modulated by Nar and hydrogen peroxide (H2O2) and TBARS decreased. Nar regulated the different pathways for scavenging of H2O2 under NaCl and/or PEG treatments. When Nar + NaCl exposure, the damage was removed by superoxide dismutase (SOD), catalase (CAT), POX (only at 0.1 mM Nar + NaCl) and AsA-GSH cycle. Under osmotic stress plus Nar, the protection was manifested by activated CAT and, glutathione S-transferase and the regeneration of ascorbate. 0.1 mM Nar could protect bean plant against salt/osmotic stresses, likely by regulating nitrogen assimilation pathways, improving expression levels of genes associated with tolerance mechanisms and modulating the antioxidant capacity and AsA-GSH redox-based systems. © 2020 Elsevier Masson SASAuthors were grateful to Bahri Dagdas International Agricultural Research Institute for providing the seeds of bean. We thank to Selcuk University Scientific Research Projects Coordinating Office (project number: 20401044) for financial support