Indifference

The matter of indifference is always relevant. Almost everyone has ever experienced it in relation to the difficulties of other people, even to the loved ones. Perhaps, a man is organized in such a way that firstly he is oriented on solving his own problems and only then on difficulties of close people. But you will not spend a lot of time and money on being responsive as it is more important to be kind, compassionate, ready to share the warmth of your soul with people in need.Тема равнодушия актуальна во все времена. Вероятно, каждый из нас хоть раз проявлял равнодушие по отношению к трудностям близких. Возможно, человек так устроен, что он склонен сначала решить собственные проблемы, а неприятности близких откладывает на второй план. Скорее всего, так мы просто пытаемся найти оправдание нашей слабости, нежеланию оказать внимание и поддержку близким людям. Отзывчивость тесно связана со способностью проявлять милосердие, сострадать. Иметь доброе сердце – не значит тратить много денег. Гораздо важнее отдавать теплоту своей души, уважать, ценить – всё это умения отзывчивого человека

Software of the Educational Process in the Preparation of Students of Secondary Vocational Education

В данной статье рассматривается вопрос необходимости составления электронных справочников по ознакомлению обучающихся с программами, которые не рассматриваются в учебном процессе, но могут существенно изменить сам процесс обучения и дополнить его.This article discusses the issue of the need to compile electronic reference books to familiarize students with programs that are not considered in the educational process, but can signifi cantly change the learning process itself and supplement it

Pulsations and Long-Term Light Variability of Three Candidates to Protoplanetary Nebulae

We present new photometric data and analysis of the long-duration UBV photoelectric observations for three candidates to protoplanetary objects - F-supergiants with IR-excesses located at large galactic latitudes, IRAS 18095+2704, IRAS 19386+0155, and IRAS 19500-1709. All three stars have revealed quasiperiodic low-amplitude variabilities caused by pulsations observed against the long-term trends of brightnesses. For IRAS 18095+2704=V887 Her we have found a pulsation period of 109 days and a linear trend of brightness under the constant colours if being averaged over the year timescale. The light curve of IRAS 19386+0155=V1648 Aql over 2000-2008 can be approximated by a wave with a main period of 102 days which is modulated by close frequency, with a period of 98 days, that results in brightness oscillations with a variable amplitude. V1648 Aql has also shown synchronous reddening together with a persistent rise of brightness in the V-band. IRAS 19500-1709=V5112 Sgr experiences irregular pulsations with the periods of 39 and 47 days. The long-term component of the variability of V5112 Sgr may be related to the binary character of this star.Comment: 11 pages, 6 figures, accepted for publication in Pis'ma Astron. Z

Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes

The lower jaws of archaeal RNA polymerase and eukaryotic RNA polymerase II include orthologous subunits H and Rpb5, respectively. The tertiary structure of H is very similar to the structure of the C-terminal domain of Rpb5, and both subunits are proximal to downstream DNA in pre-initiation complexes. Analyses of reconstituted euryarchaeal polymerase lacking subunit H revealed that H is important for open complex formation and initial transcription. Eukaryotic Rpb5 rescues activity of the ΔH enzyme indicating a strong conservation of function for this subunit from archaea to eukaryotes. Photochemical cross-linking in elongation complexes revealed a striking structural rearrangement of RNA polymerase, bringing subunit H near the transcribed DNA strand one helical turn downstream of the active center, in contrast to the positioning observed in preinitiation complexes. The rearrangement of subunits H and A′′ suggest a major conformational change in the archaeal RNAP lower jaw upon formation of the elongation complex

Influence of Electric Explosive Doping with Yttrium and Electron Beam Treatment on the Structure and Phase Composition of Titanium

The purpose of this article was to identify the features of the formation of the structure and phase composition of technically pure titanium in EVL with yttrium and subsequent EPO. Methods of modern materials science conducted studies of the structure, phase composition, mechanical properties of titanium VT1–0 after combined treatment.Целью настоящей статьи являлось выявление особенностей формирования структуры и фазового состава технически чистого титана при ЭВЛ иттрием и последующей ЭПО. Методами современного материаловедения проводились исследования структуры, фазового состава, механических свойств титана ВТ1–0 после комбинированной обработки

Spectrum of mutations and their phenotypic manifestations in children and adults with long QT syndrome

Aim. To determine the spectrum of mutations in the genes responsible for the long QT syndrome (LQTS) and study their phenotypic manifestations in patients with LQTS in different age groups.Materials and methods. The study included 35 unrelated probands with a clinical diagnosis of LQTS: 23 adults (8 men) and 12 children (9 boys). There were following clinical features: syncope — 54%, positive family history for SCD — 29%, implanted cardioverter defibrillator (ICD) — 46%. All participants underwent 12-lead electrocardiography (ECG), 24-hour Holter monitoring, genealogical analysis, echocardiography and cardiac MRI. The genetic study was performed by nextgeneration sequencing (NGS) using the MiSeq system (Illumina). The quantitative comparison of two unrelated groups was carried out using the nonparametric MannWhitney U-test. The differences were considered significant at p<0,05.Results. In the examined group of 35 probands, 23 genetic variants of pathogenicity class IV and V (hereinafter referred to as) were identified. The molecular genetic variant of the disease was verified in 66% of probands. At the same time, the detection of mutations in the group with early manifestation (children) was significantly higher: 83% (10 out of 12 children) vs 57% in adults (13 out of 23). Rare genetic variants of uncertain significance (VUS, class III pathogenicity) were detected in 4 probands (11%). In the groups of children and adults with LQT1, LQT2 and LQT3, the sex distribution deviated from the 1:1 ratio. Among children, two-thirds were boys, among adults — the same proportion was represented by women. Disease manifestation time, QTc duration and adverse events risk depended on the genetic type of LQTS, intragenic localization of mutations and sex. In children, all 4 missense mutations in the KCNQ1 gene were located in transmembrane domain, and in adults, 4 mutations were in the transmembrane domain and three — in the C-terminal domain of the protein. LQT1 in boys was characterized by early manifestation, while QTc did not exceed 500 ms and there were no adverse outcomes. Two women out of 7 adults with LQT1 with mutations in the transmembrane domain had na ICD (QTc >520 ms). All patients with LQT2 (4 children, 4 adults) had QTc >500 ms. At the same time, 2 children and 3 women had an ICD. LQT3 was diagnosed only in the children subgroup (2 boys, with QTc of 510 ms and QTc of 610 ms); one of them died suddenly despite beta-blocker therapy. Four adult patients, carriers of class III pathogenicity variants, had QTc <500 ms and delayed disease manifestation (after 30 years). Three of them had episodes of clinical death with subsequent resuscitation and implantation of cardioverter defibrillator.Conclusion. The average diagnostic efficiency of mutation identification using NGS in patients with clinically manifest LQTS was 66%. At the same time, mutations were more common in the children’s group. In genotype-positive probands, the risk of adverse outcomes correlated with sex, age and the genetic variant of disease. The greatest number of adverse outcomes was observed in carriers of mutations in both KCNH2 (LQT2) and SCN5A (LQT3) genes. Variants with unknown clinical significance were identified in 4 probands (11%), which potentially allowed to confirm the diagnosis after functional tests

Efficacy of treatment of newly detected patients with MDR tuberculosis in early detection of drug resistance of M.tuberculosis to rifampicin

We studied 261 patients with newly detected MDR tuberculosis who were divided into 2 groups. The first group consisted of 164 patients in whom, before hospitalization determined the resistance of M. tuberculosis to rifampicin by the GeneXpert MTB/RIF method. Treatment of this group of patients was initially conducted according to the 4th regimen of chemotherapy (pyrazinamide, kanamycin/amk/capreomycin, fluoroquinolone, cycloserine/terizidone, protionamide, PAS). The second group (the comparison group) included 97 patients in whom MDR was diagnosed by sputum culture method. Prior to receiving data on the drug resistance, these patients received treatment according to the 1st standard chemotherapy regimen (isoniazid, rifampicin, pyrazinamide, ethambutol). After detecting MDR M. tuberculosis (after 2-3 months of treatment) correction treatment with reregistration for 4th regimens were performed. It was found that the new detected MDR tuberculosis in the Stavropol region of Russian Federation is more often detected in men in the form of infiltrative tuberculosis, characterized by the M. tuberculosis positive sputum and destruction in lung tissue up to 2 cm in size. The efficacy of treatment by conversation sputum culture after 3 and 6 months of treatment, as well as to close cavities after 6 months of treatment in the group of patients initially treated with 4 regimens of chemotherapy was significantly higher in comparison with patients initially treated with 1 and then 4 chemotherapy regimens.Под нашим наблюдением находился 261 больной с различными клиническими формами впервые выявленного МЛУ туберкулеза легких, которые были разделены на 2 группы. В первую (основную – «4 режим ХТ») группу вошли 164 больных, у которых при поступлении в стационар методом GeneXpert MTB/RIF определялась устойчивость МБТ к рифампицину. Лечение этой группы больных исходно проводилось по 4-му режиму химиотерапии (ХТ) пиразинамид, канамицин /амикацин/капреомицин, левофлоксацин, циклосерин/теризидон, протионамид, ПАСК). Во вторую группу (группа сравнения – «1/4 режим ХТ») включили 97 больных, у которых МЛУ МБТ диагностировалась методом посева мокроты на плотные питательные среды. до получения данных лекарственной устойчивости МБТ этим больным назначали лечение по 1-му стандартному режиму химиотерапии (изониазид, рифампицин, пиразинамид, этамбутол). После получения данных о МЛУ МБТ (через 2-3 месяца лечения) проводилась коррекция лечения с перерегистрацией на 4 режим ХТ. Было установлено, что впервые выявленный МЛУ туберкулез легких чаще выявлялся у мужчин в виде инфильтративного туберкулеза, характеризовался наличием бактериовыделения и распадом в легочной ткани размером до 2 см. Эффективность лечения впервые выявленного МЛУ туберкулеза легких по прекращению бактериовыделения методом посева мокроты через 3 и 6 месяцев лечения, а также по закрытию полостей распада через 6 месяцев лечения в группе больных, изначально лечившихся 4 режимом химиотерапии была достоверно выше по сравнению с пациентами, исходно леченных 1, а затем 4 режимом химиотерапии

УРОВЕНЬ МОЧЕВОЙ КИСЛОТЫ В СЫВОРОТКЕ КРОВИ У БОЛЬНЫХ ВПЕРВЫЕ ВЫЯВЛЕННЫМ ТУБЕРКУЛЕЗОМ ЛЕГКИХ С МНОЖЕСТВЕННОЙ ЛЕКАРСТВЕННОЙ УСТОЙЧИВОСТЬЮ ВОЗБУДИТЕЛЯ

The level of uric acid in blood serum was investigated in 134 new patients suffering from multiple drug resistant pulmonary tuberculosis (78 men and 56 women). The tests were performed by admission to hospital and then repeated in 1, 2, 3, 4, 5, 6, 7 and 8 months of treatment. Prior to chemotherapy the increased uric acid level was observed in 28.2% of men and 37.5% of women. During treatment with pyrazinamide uric acid level increased in this category of patients in 1 month after treatment start and further it went down; however it was above normal during the whole course of treatment. Monitoring of uric acid level in the patients with initially normal rates has shown that the primary increase of uric acid was observed during the whole course of chemotherapy (8 months) and more often during the first 2 months of treatment both in men and women.Уровень мочевой кислоты (МК) в сыворотке крови изучен у 134 больных впервые выявленным туберкулезом легких с множественной лекарственной устойчивостью возбудителя (78 мужчин и 56 женщин). Исследование проводили при поступлении больных в клинику и затем повторно – через 1, 2, 3, 4, 5, 6, 7 и 8 мес. лечения. Повышение уровня МК до начала химиотерапии наблюдалось у 28,2% мужчин и у 37,5% женщин. В процессе лечения пиразинамидом уровень МК у пациентов этой категории возрастал через 1 мес. лечения, а в дальнейшем начинал снижаться, однако на протяжении всего периода наблюдения превышал нормальные значения. Мониторинг уровня МК у больных с исходно нормальными значениями показал, что первичное повышение уровня МК наблюдалось на протяжении всего периода (8 мес.) химиотерапии, чаще всего в период первых 2 мес. лечения, как у мужчин, так и у женщин

Efficacy of a shorter chemotherapy regimen in a patient with drug-resistant tuberculosis with early surgical intervention

The purpose of the study was to demonstrate the high efficacy of a shortened chemotherapy regimen with surgical intervention.Цель работы – продемонстрировать высокую эффективность укороченного режима химиотерапии с применением оперативного вмешательства