Serological methods in modern biotechnologies and their bioanalytical standardization

Серологічні методи використовувались в першу чергу перед усіма методами клінічної лабораторної діагностики, і вони залишаються надзвичайно актуальними і донині. Серологічні методи застосовуються для діагностики як інфекційних (бактеріальних, вірусних, грибкових, паразитарних), так і неінфекційних (онкологічних, ендокринних, алергічних) захворювань. Значна частка діагностичних обстежень, проведених лабораторною службою, стосується саме серологічних тестів. Серологічні методи залишаються невід'ємною частиною забезпечення санітарного та епідеміологічного благополуччя кожної країни. Розробка продуктів серологічної діагностики є предметом імунобіотехнології – розділу біотехнології, метою якого є створення діагностичних та імунних препаратів для діагностики, лікування та профілактики захворювань людини та тварин. Продукти для серологічної діагностики належать до класу медичних апаратів. Очевидно, що проблема, вирішена такими медичними препаратами, є надзвичайно важливою, адже від вірогідності результатів лабораторного дослідження залежить здоров’я та життя пацієнта. Ось чому на сучасному етапі особлива увага приділяється якості медичних пристроїв для діагностики in vitro, що, в свою чергу, пов'язано з питаннями стандартизації та технічного регулювання. У роботі здійснено огляд медикобіологічних характеристик основних поширених серологічних методів діагностики. Узагальнено сучасні наукові та нормативні вимоги до аналітичної якості продукції для серологічної діагностики in vitro.Serological methods have become to use first turn before all methods of clinical laboratory diagnostics, and they stay remain extremely relevant to the present day. Serologic methods are used for diagnostics as infectious (bacterial, viral, fungal, parasitic), and non-infectious (oncological, endocrine, allergic) diseases. A significant proportion of diagnostic examinations carried out by the laboratory service relates precisely to serological tests. Serologic methods remain an indispensable part of ensuring the sanitary and epidemiological well-being of each country. Development of serological diagnostics products is the subject of immunobiotechnology – a section of biotechnology, which aims to create diagnostic and immune preparations for the diagnosis, treatment and prevention of human and animal diseases. Products for serological diagnostics belong to a class of medical devises. Obviously, the problem solved by such medical products is extremely responsible, because the health and life of the patient depends on the correctness of the outcome of the laboratory study. That is why, at the present stage, special attention is paid to the quality of medical devises for in vitro diagnostics, which, in turn, is connected with questions of standardization and technical regulation. A review of the medical and biological characteristics of the main common serological methods of diagnosis was made. The modern scientific and regulatory requirements for the analytical quality of products for serological in vitro diagnostics were summarized

Type And Material of Fixed Prosthodontic Appliances in Patients Living in the Region of Metković

The aim of this study was to evaluate the type and the aesthetic material in relation to age, gender, level of education, employment, socio-economic status and frequency of visits to the dentist. The examination was performed on 212 patients who had a fixed prosthodontic appliance for more than a year (55% males and 45% females, age 18-80 yrs.). The following conclusions were made: 1. The older patients and retired patients had significantly more bridges than crowns (p0.05). Patients who visit their dentist regularly have significantly more crowns than bridges than patients who visit their dentist irregularly or when in pain (p0.05). 2. Almost all fixed prosthodontic appliances older than 10 years were made of porcelain (98%), while acrylic veneer crowns were more frequent in appliances older than 10 or 15 years (p0.05). Patients older than 60 years had more acrylic material compared to younger patients. While patients younger than 39 years had almost exclusively ceramic appliances (p<0.01). Less educated patients had more acrylic veneer appliances. Employed patients had significantly more ceramic appliances than retired patients

Symmetry, singularities and integrability in complex dynamics III: approximate symmetries and invariants

The different natures of approximate symmetries and their corresponding first integrals/invariants are delineated in the contexts of both Lie symmetries of ordinary differential equations and Noether symmetries of the Action Integral. Particular note is taken of the effect of taking higher orders of the perturbation parameter. Approximate symmetries of approximate first integrals/invariants and the problems of calculating them using the Lie method are considered

Electromagnetic Energy for a Charged Kerr Black Hole in a Uniform Magnetic Field

With the Komar mass formula we calculate the electromagnetic energy for a charged Kerr black hole in a uniform magnetic field. We find that the total electromagnetic energy takes the minimum when the Kerr black hole possesses a non-zero net charge $Q = 2\xi B_0 J_H$ where $B_0$ is the strength of the magnetic field, $J_H$ is the angular momentum of the black hole, $\xi$ is a dimensionless parameter determined by the spin of the black hole.Comment: 9 pages, 1 figur

Analysis of Occlusal Contacts in Different Types of Prosthodontic Appliances, Eichner Classifications, Presence RCP-ICP Slide and the Type of Occlusion

The aim of the study was to determine the number and the type of the occlusal contacts (strong, weak) with respect to the type of the prosthodontic appliance (fixed, removable, combined fixed-removable) and with respect to the Eichner classification in patients with their appliances being in a good function for a long time. The aim of the study was also to determine the number and the type of the occlusal contacts (strong, weak) with respect to the type of occlusion (canine guided, group function or balanced) and the presence of the RCP-ICP slide. A total of 440 patients with different types of prosthodontic appliances were examined for the antagonistic occlusal contacts using occlusal strips of 11 m and 50 m. The average number of occlusal contacts was 10.5 for the upper and 10.46 for the lower posterior teeth, approximately 5 on each side of the tooth arch. The results of the study suggest that the biggest number of occlusal contacts were recorded for the small span fixed appliances (2 on average), the greater span fixed and fixed-removable prosthodontic appliances exhibited 1.6 occlusal contacts, and the removable complete denture exhibited 1.2 contact per the tooth in the posterior region. The number of the hard occlusal contacts was significantly greater in fixed and fixedremovable prosthodontic appliances in comparison with the complete dentures (p < 0.05), while there was no significant difference between the prosthodontic appliances for the weak occlusal contacts (p > 0.05). The overall number of the occlusal contacts, as well as the number of the hard occlusal contacts was significantly greater in the Eichner class I cases (p < 0.05) in comparison with the Eichner classes II and III. The number of the weak occlusal contacts showed no significant differences with respect to the Eichner classification (p > 0.05). There was no significant difference in the number of occlusal contacts between the appliances with RCP-ICP slide and where ICP and RCP corresponded (p > 0.05)

Methodical instructions for performing practical work on the subject "Operation and maintenance of machines" for students of all forms of studyDirection of preparation 131 " Applied mechanics"

1. DETERMINATION OF THE LENGTH OF REPAIR CYCLES AND REPAIR PERIODS OF EQUIPMENT OF THE MACHINE-BUILDING ENTERPRISE 5 2. DETERMINING THE CONJUGATION RESOURCE 9 3. DETERMINATION OF THE CRITERIA FOR THE STABILITY OF THE TECHNOLOGICAL PROCESS AND ENSURING THE ACCURACY OF MACHINING ON THE LATHE THROUGH RATIONAL TUNING 18 4. DETERMINATION OF FORCES ON THE HANDLES OF MOVEMENT AND CLAMPING OF ORGANS, BACKLASHES, ACCURACY OF MOVEMENTS ALONG THE LIMBS, THEIR CALCULATION 24 5. EFFECT OF CLAMPING FORCE ON PROCESSING ACCURACY 34 6. THE INFLUENCE OF TEMPERATURE DEFORMATIONS ON THE ACCURACY OF PROCESSING PARTS 41 7. DETERMINATION OF THE DEVIATION FROM THE STRAIGHTNESS OF THE MOVEMENT OF THE WORKING BODY IN A GIVEN PLANE 44 8. LITERATUR

Diffeomorphisms, Noether Charges and Canonical Formalism in 2D Dilaton Gravity

We carry out a parallel study of the covariant phase space and the conservation laws of local symmetries in two-dimensional dilaton gravity. Our analysis is based on the fact that the Lagrangian can be brought to a form that vanishes on-shell giving rise to a well-defined covariant potential for the symplectic current. We explicitly compute the symplectic structure and its potential and show that the requirement to be finite and independent of the Cauchy surface restricts the asymptotic symmetries.Comment: 14 pages, latex with psfig macro, one figur

Schwarzschild black hole levitating in the hyperextreme Kerr field

The equilibrium configurations between a Schwarzschild black hole and a hyperextreme Kerr object are shown to be described by a three-parameter subfamily of the extended double-Kerr solution. For this subfamily, its Ernst potential and corresponding metric functions, we provide a physical representation which employs as arbitrary parameters the individual Komar masses and relative coordinate distance between the sources. The calculation of horizon's local angular velocity induced in the Schwarzschild black hole by the Kerr constituent yields a simple expression inversely proportional to the square of the distance parameter.Comment: 6 pages, 1 figure; improved versio

Quasigroups, Asymptotic Symmetries and Conservation Laws in General Relativity

A new quasigroup approach to conservation laws in general relativity is applied to study asymptotically flat at future null infinity spacetime. The infinite-parametric Newman-Unti group of asymptotic symmetries is reduced to the Poincar\'e quasigroup and the Noether charge associated with any element of the Poincar\'e quasialgebra is defined. The integral conserved quantities of energy-momentum and angular momentum are linear on generators of Poincar\'e quasigroup, free of the supertranslation ambiguity, posess the flux and identically equal to zero in Minkowski spacetime.Comment: RevTeX4, 5 page