Clinical determinants of thirty-day mortality in a cohort of patients with severe alcoholic hepatitis

Background: Aim and objectives was to study clinical profile of patients with severe alcoholic hepatitis (SAH) and evaluate clinical factors associated with short term (30-day) mortality.Methods: This is a prospective study conducted from January 2016 to January 2017 at Liver Care Unit, Osmania General Hospital. This study was approved by ethics committee of the hospital and written informed consent was obtained from all subjects included in the study. Patients with clinical alcoholic hepatitis with serum bilirubin >5mg/dl, aspartate amino transferase (AST)/ alanine amino transferase (ALT) ratio >2 with an AST level >45 but <500U/L, Maddrey’s Discriminant function (MDF) ≥32 were included in the study.Results: The 30-day mortality of severe AH in the current study was 40%. Alcoholic hepatitis was most common in males between 40-50 years with a median age of 46.9±7.7 (31-60) years. The clinical complications consisted of hepatic encephalopathy (HE) in 40%, hepato renal syndrome (HRS) and renal failure in 18.2% and infections in 40%. HRS, bilirubin, ALT, AST, urea, creatinine, Na+ and all prognostic scores showed significant association with in hospital mortality at 30days on univariate analysis while United Kingdom end liver disease (UKELD) and Child-Turcotte-Pugh (CTP) scores showed most significance on multivariate regression analysis.Conclusions: The 30-day mortality of severe AH in the current study was 40%. High UKELD, CTP scores and presence of HRS/Renal dysfunction at time of admission are associated with high 30-day mortality. Patients with advanced age, decompensated cirrhosis, coagulopathy, renal injury, malnourished status and low sodium respond poorly to therapy

Genetic variation of trypsin and chymotrypsin inhibitors in pigeonpea [Cajanus cajan (L.) Millsp.] and its wild relatives

Variation in the trypsin inhibitors (TIs) and the chymotrypsin inhibitors (CIs) among 69 pigeonpea [Cajanus cajan (L.) Millsp.] strains from a wide geographical distribution and among 17 accessions representing seven wild Cajanus species was studied by electrophoretic banding pattern comparisons and by spectrophotometric activity assays. The TI and CI electrophoretic migration patterns among the pigeonpea strains were highly uniform but varied in the inhibitor band intensities. The migration patterns of the inhibitors in the wild Cajanus species were highly species specific. The mean TI activity of pigeonpea strains (2279 units) was significantly higher than that of the wild Cajanus species (1407 units). However, the mean CI activity in the pigeonpea strains (62 units) was much lower than that in the wild species (162 units). Kenya 2 and ICP 9151 were the lowest and the highest, respectively, in both the TI and CI activities among all the pigeonpea strains used in this study. A highly-significant positive correlation was observed between the TI and CI activities. The Bowman-Birk type inhibitors with both TI and CI activities were identified in all the pigeonpea strains and also in the accessions of all the wild species except C. volubilis (Blanco) Blanco. The C. volubilis accession ICPW 169 was found to be ‘null’ for both CI bands and CI activity. Environment, strain, and environment x strain interaction showed highly-significant effects on both the TI and CI activities. Growing the pigeonpea strains at a different environment from their area of adaptation increased TI and CI activities and also altered the maturity perio

Time Domain Explorations With Digital Sky Surveys

One of the new frontiers of astronomical research is the exploration of time variability on the sky at different wavelengths and flux levels. We have carried out a pilot project using DPOSS data to study strong variables and transients, and are now extending it to the new Palomar-QUEST synoptic sky survey. We report on our early findings and outline the methodology to be implemented in preparation for a real-time transient detection pipeline. In addition to large numbers of known types of highly variable sources (e.g., SNe, CVs, OVV QSOs, etc.), we expect to find numerous transients whose nature may be established by a rapid follow-up. Whereas we will make all detected variables publicly available through the web, we anticipate that email alerts would be issued in the real time for a subset of events deemed to be the most interesting. This real-time process entails many challenges, in an effort to maintain a high completeness while keeping the contamination low. We will utilize distributed Grid services developed by the GRIST project, and implement a variety of advanced statistical and machine learning techniques.Comment: 5 pages, 2 postscript figures, uses adassconf.sty. To be published in: "ADASS XIV (2004)", Eds. Patrick Shopbell, Matthew Britton and Rick Ebert, ASP Conference Serie

Protease inhibitors of Cajanus conferring resistance to pod borer of pigeonpea (Cajanus cajan L. Millsp.).

Pigeonpea is susceptible to pod borer damage with resistance lacking in its primary gene pool. Many Cajanus species harbor high levels of resistance. Host plant resistance can play an important role in minimizing the extent of losses due to insects and pests as well as the use of insecticides/pesticides and thus protect the environment. A major initiative was undertaken to tap the defence genes from wild relatives of secondary and tertiary gene pool through wide hybridization and thereby introgress resistance to pod borer. A range of interspecific derivatives derived from C. lanceolatus, C. cajanifolius, C. volubilis and C. platycarpus along with their parents were screened for the pod borer resistance under unprotected field conditions at ICRISAT, Patancheru, India. Biochemical basis of resistance was also identified by studying the levels of defence proteins active against bovine pancreatic trypsin, chymotrypsin and trypsin-like enzymes of H. armigera mid-gut proteases. Protease inhibitor profiles of parents and interspecific derivatives differed in terms of activity units, number and intensities of activity bands visualized on gelatin-PAGE. As the protease inhibitors are anti-nutritional factors, parents and interspecific derivatives, which resulted in high levels of Helicoverpa gut protease inhibitor (HGPI) units were screened for Human pancreatic trypsin inhibitor (HPTI) activity levels. Samples with high ratio of HGPI/HPTI represent less or no effect on human pancreatic trypsin and high effect on insect gut proteases

Implementation of Transmission Line Fault Detection System using Long Range Wireless Sensor Networks

This paper proposes a fault detection system designed for transmission lines using Long-Range Wireless Sensor Network (LoRAWSN). The system is designed to detect and locate faults across transmission lines in real-time, which can significantly improve the reliability and efficiency of power transmission systems. A WSN will be built across transmission lines over an area. The faults identified by these sensor nodes is then transmitted to a central control unit, which analyses and displays the data. The LoRaWAN technology enables the WSN to cover long distances while consuming minimal power, making it ideal for monitoring transmission lines. The proposed fault detection system is evaluated through real world experiments, which demonstrate the feasibility and effectiveness of the proposed system. Overall, this paper presents a novel and practical approach for fault detection on transmission lines, which has the potential to improve the reliability and efficiency of power transmission systems

Chronic testicular Chlamydia muridarum infection impairs mouse fertility and offspring development

With approximately 131 million new genital tract infections occurring each year, Chlamydia is the most common sexually transmitted bacterial pathogen worldwide. Male and female infections occur at similar rates and both cause serious pathological sequelae. Despite this, the impact of chlamydial infection on male fertility has long been debated, and the effects of paternal chlamydial infection on offspring development are unknown. Using a male mouse chronic infection model, we show that chlamydial infection persists in the testes, adversely affecting the testicular environment. Infection increased leukocyte infiltration, disrupted the blood:testis barrier and reduced spermiogenic cell numbers and seminiferous tubule volume. Sperm from infected mice had decreased motility, increased abnormal morphology, decreased zona-binding capacity, and increased DNA damage. Serum anti-sperm antibodies were also increased. When both acutely and chronically infected male mice were bred with healthy female mice, 16.7% of pups displayed developmental abnormalities. Female offspring of chronically infected sires had smaller reproductive tracts than offspring of noninfected sires. The male pups of infected sires displayed delayed testicular development, with abnormalities in sperm vitality, motility, and sperm-oocyte binding evident at sexual maturity. These data suggest that chronic testicular Chlamydia infection can contribute to male infertility, which may have an intergenerational impact on sperm quality

The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier

Podocytes form the outer part of the glomerular filter, where they have to withstand enormous transcapillary filtration forces driving glomerular filtration. Detachment of podocytes from the glomerular basement membrane precedes most glomerular diseases. However, little is known about the regulation of podocyte adhesion in vivo. Thus, we systematically screened for podocyte-specific focal adhesome (FA) components, using genetic reporter models in combination with iTRAQ-based mass spectrometry. This approach led to the identification of FERM domain protein EPB41L5 as a highly enriched podocyte-specific FA component in vivo. Genetic deletion of Epb41l5 resulted in severe proteinuria, detachment of podocytes, and development of focal segmental glomerulosclerosis. Remarkably, by binding and recruiting the RhoGEF ARGHEF18 to the leading edge, EPB41L5 directly controls actomyosin contractility and subsequent maturation of focal adhesions, cell spreading, and migration. Furthermore, EPB41L5 controls matrix-dependent outside-in signaling by regulating the focal adhesome composition. Thus, by linking extracellular matrix sensing and signaling, focal adhesion maturation, and actomyosin activation EPB41L5 ensures the mechanical stability required for podocytes at the kidney filtration barrier. Finally, a diminution of EPB41L5-dependent signaling programs appears to be a common theme of podocyte disease, and therefore offers unexpected interventional therapeutic strategies to prevent podocyte loss and kidney disease progression

CANDELS+3D-HST: Compact SFGs at \u3cem\u3ez\u3c/em\u3e ~ 2-3, the Progenitors of the First Quiescent Galaxies

We analyze the star-forming and structural properties of 45 massive (log(M/M ☉) \u3e10) compact star-forming galaxies (SFGs) at 2 \u3c z \u3c 3 to explore whether they are progenitors of compact quiescent galaxies at z ~ 2. The optical/NIR and far-IR Spitzer/Herschel colors indicate that most compact SFGs are heavily obscured. Nearly half (47%) host an X-ray-bright active galactic nucleus (AGN). In contrast, only about 10% of other massive galaxies at that time host AGNs. Compact SFGs have centrally concentrated light profiles and spheroidal morphologies similar to quiescent galaxies and are thus strikingly different from other SFGs, which typically are disk-like and sometimes clumpy or irregular. Most compact SFGs lie either within the star formation rate (SFR)-mass main sequence (65%) or below it (30%), on the expected evolutionary path toward quiescent galaxies. These results show conclusively that galaxies become more compact before they lose their gas and dust, quenching star formation. Using extensive HST photometry from CANDELS and grism spectroscopy from the 3D-HST survey, we model their stellar populations with either exponentially declining (τ) star formation histories (SFHs) or physically motivated SFHs drawn from semianalytic models (SAMs). SAMs predict longer formation timescales and older ages ~2 Gyr, which are nearly twice as old as the estimates of the τ models. Both models yield good spectral energy distribution fits, indicating that the systematic uncertainty in the age due to degeneracies in the SFH is of that order of magnitude. However, SAM SFHs better match the observed slope and zero point of the SFR-mass main sequence. Contrary to expectations, some low-mass compact SFGs (log(M/M ☉) =10-10.6) have younger ages but lower specific SFRs than that of more massive galaxies, suggesting that the low-mass galaxies reach the red sequence faster. If the progenitors of compact SFGs are extended SFGs, state-of-the-art SAMs show that mergers and disk instabilities (DIs) are both able to shrink galaxies, but DIs are more frequent (60% versus 40%) and form more concentrated galaxies. We confirm this result via high-resolution hydrodynamic simulations

Taxane-Platin-Resistant Lung Cancers Co-develop Hypersensitivity to JumonjiC Demethylase Inhibitors

Although non-small cell lung cancer (NSCLC) patients benefit from standard taxane-platin chemotherapy, many relapse, developing drug resistance. We established preclinical taxane-platin-chemoresistance models and identified a 35-gene resistance signature, which was associated with poor recurrence-free survival in neoadjuvant-treated NSCLC patients and included upregulation of the JumonjiC lysine demethylase KDM3B. In fact, multi-drug-resistant cells progressively increased the expression of many JumonjiC demethylases, had altered histone methylation, and, importantly, showed hypersensitivity to JumonjiC inhibitors in vitro and in vivo. Increasing taxane-platin resistance in progressive cell line series was accompanied by progressive sensitization to JIB-04 and GSK-J4. These JumonjiC inhibitors partly reversed deregulated transcriptional programs, prevented the emergence of drug-tolerant colonies from chemo-naive cells, and synergized with standard chemotherapy in vitro and in vivo. Our findings reveal JumonjiC inhibitors as promising therapies for targeting taxane-platin-chemoresistant NSCLCs.Fil: Dalvi, Maithili P.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Wang, Lei. University of Texas. Southwestern Medical Center; Estados UnidosFil: Zhong, Rui. University of Texas. Southwestern Medical Center; Estados UnidosFil: Kollipara, Rahul K.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Park, Hyunsil. University of Texas. Southwestern Medical Center; Estados UnidosFil: Bayo Fina, Juan Miguel. University of Texas. Southwestern Medical Center; Estados Unidos. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaFil: Yenerall, Paul. University of Texas. Southwestern Medical Center; Estados UnidosFil: Zhou, Yunyun. University of Texas. Southwestern Medical Center; Estados UnidosFil: Timmons, Brenda C.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Rodriguez Canales, Jaime. University of Texas; Estados UnidosFil: Behrens, Carmen. Md Anderson Cancer Center; Estados UnidosFil: Mino, Barbara. University of Texas; Estados UnidosFil: Villalobos, Pamela. University of Texas; Estados UnidosFil: Parra, Edwin R.. University of Texas; Estados UnidosFil: Suraokar, Milind. University of Texas; Estados UnidosFil: Pataer, Apar. University of Texas; Estados UnidosFil: Swisher, Stephen G.. University of Texas; Estados UnidosFil: Kalhor, Neda. University of Texas; Estados UnidosFil: Bhanu, Natarajan V.. University of Pennsylvania; Estados UnidosFil: Garcia, Benjamin A.. University of Pennsylvania; Estados UnidosFil: Heymach, John V.. University of Texas; Estados UnidosFil: Coombes, Kevin. University of Texas; Estados UnidosFil: Xie, Yang. University of Texas. Southwestern Medical Center; Estados UnidosFil: Girard, Luc. University of Texas. Southwestern Medical Center; Estados UnidosFil: Gazdar, Adi F.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Kittler, Ralf. University of Texas. Southwestern Medical Center; Estados UnidosFil: Wistuba, Ignacio I.. University of Texas; Estados UnidosFil: Minna, John D.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Martinez, Elisabeth D.. University of Texas. Southwestern Medical Center; Estados Unido