Cytotoxic Effect Of Boron Aplication On Glioblastoma Cells

AIM: To investigate the cytotoxic effects of boron application at different doses on U-87 MG glioblastoma cells

Relationship of Bradykinin B-2 Receptor Gene C-58T Variation with Total-Cholesterol and Glucose in Essential Hypertension

Objective: Hypertension, which affects 25% of the world's population, is a complex disease, with 30% genes affecting its pathogenesis. In this study, we aimed to examine the frequency of the bradykinin B-2 receptor gene C-58T genotypes in patients with essential hypertension and non-hypertensive controls. In addition, we evaluated the effects of C-58T genotypes on demographic characteristics, phenotypes related to obesity, and hypertension

Association of Sweet Taste Receptor Gene Polymorphisms with Dental Caries Experience in School Children

Sweet taste is a powerful factor influencing food acceptance. The peripheral taste response to sugar is mediated by the TAS1R2/TAS1R3 taste receptors. The aim of the study was to determine the relationship between TAS1R2 (rs35874116 or rs9701796) and/or TAS1R3 (rs307355) single nucleotide polymorphisms with dental caries experience in schoolchildren. A total of 184 schoolchildren aged between 7 and 12 years (101 girls, 83 boys) were included in the study. Genomic DNA was extracted from saliva samples and the genotypes were identified by qPCR. The genotype frequencies were as follows: 6.6% for homozygous wild type, 41.8% for heterozygous and 51.6% for homozygous polymorphic genotype carriers of TAS1R2 gene rs35874116; 27.8% for heterozygous and 72.2% for homozygous polymorphic genotype carriers of TAS1R2 gene rs9701796, and 83.1% for homozygous wild type and 16.9% for heterozygous genotype carriers of TAS1R3 gene rs307355 polymorphism. A significant association was observed between total caries experience (dft + DMFT - decayed filled primary teeth + decayed, missing and filled permanent teeth) and TAS1R2 rs35874116 (p = 0.008) and TAS1R3 rs307355 (p = 0.04) gene polymorphisms but not for TAS1R2 gene rs9701796 polymorphism. TAS1R3 gene rs307355 polymorphism has been found to be an independent risk factor for dental caries experience by logistic regression analysis and to have increased the risk of caries. Moderate caries experience (4-7 caries) was found to be associated with TAS1R3 rs307355 heterozygous genotype, whereas high-risk caries experience (>8 caries) was found to be associated with TAS1R2 rs35874116 homozygous polymorphic genotype. (C) 2015 S. Karger AG, Base

Association of Sweet Taste Receptor Gene Polymorphisms with Dental Caries Experience in School Children

Sweet taste is a powerful factor influencing food acceptance. The peripheral taste response to sugar is mediated by the TAS1R2/TAS1R3 taste receptors. The aim of the study was to determine the relationship between TAS1R2 (rs35874116 or rs9701796) and/or TAS1R3 (rs307355) single nucleotide polymorphisms with dental caries experience in schoolchildren. A total of 184 schoolchildren aged between 7 and 12 years (101 girls, 83 boys) were included in the study. Genomic DNA was extracted from saliva samples and the genotypes were identified by qPCR. The genotype frequencies were as follows: 6.6% for homozygous wild type, 41.8% for heterozygous and 51.6% for homozygous polymorphic genotype carriers of TAS1R2 gene rs35874116; 27.8% for heterozygous and 72.2% for homozygous polymorphic genotype carriers of TAS1R2 gene rs9701796, and 83.1% for homozygous wild type and 16.9% for heterozygous genotype carriers of TAS1R3 gene rs307355 polymorphism. A significant association was observed between total caries experience (dft + DMFT - decayed filled primary teeth + decayed, missing and filled permanent teeth) and TAS1R2 rs35874116 (p = 0.008) and TAS1R3 rs307355 (p = 0.04) gene polymorphisms but not for TAS1R2 gene rs9701796 polymorphism. TAS1R3 gene rs307355 polymorphism has been found to be an independent risk factor for dental caries experience by logistic regression analysis and to have increased the risk of caries. Moderate caries experience (4-7 caries) was found to be associated with TAS1R3 rs307355 heterozygous genotype, whereas high-risk caries experience (>8 caries) was found to be associated with TAS1R2 rs35874116 homozygous polymorphic genotype. (C) 2015 S. Karger AG, Base

The Effects of Peroxiredoxin 6 Gene rs41055489 Variation on Oxidative Stress Mechanisms in Human Model Organism

Objective: Peroxiredoxins (Prx) belong to peroxidase type enzyme group which reduce redox active cysteine residues. The aim of this study was to determine the allele frequencies of Prx 6 gene rs41055489 polymorphism in zebrafish and in which extent may improve protective measures against oxidative stress indicators

THE SUBLETHAL DISRUPTING EFFECTS OF FLUOXETINE-HCI (FLX) ON CATALASE (CAT) ACTIVITY AND MALONDIALDEHYDE (MDA) LEVELS IN ZEBRAFISH, DANIO RERIO

The aim of this study is to investigate sub-lethal disrupting effects of fluoxetine-HCl (FLX) on catalase (CAT) activity and malondialdehyde (MDA) levels of liver tissue and whole body in zebrafish (Danio rerio). FLX is the active compound of the antidepressant Prozac(TM) and acts as a selective serotonin reuptake inhibitor (SSRI) in humans. Zebrafish are well-characterized model organism especially for the antioxidant enzyme activities for humans. Our study was planned as a model to investigate the effects of daily intake doses of FLX, which adapted from human proportionally to weight, on liver tissue of zebrafish. CAT, MDA and total protein levels were detected using spectrophotometric methods. The six experiment study groups were composed as; 150 ng fluoxetine-HCl exposed to each aquarium tank and five zebrafish were studied at 15 min., 30 min., 60 min., 4 days and 8 days of exposure and the last group was composed as the control group. In our study, it is determined that the activity of CAT increases in two experimental group after exposure FLX (15 min. and 30 min.) in liver tissue. Following one hour FLX treatment, it was observed that CAT activity decreased, whereas after four days treatment it was re-increased. Once for all, following 8 days treatment of FLX, it was observed that CAT activity significantly decreased. Likewise we found that the MDA levels decreases in all experimental groups after exposure FLX in liver tissue definitely. In whole body groups, MDA levels was firstly decreased after exposure FLX but MDA levels was increased subsequently compared to the control group in this study. In conclusion, building on the framework surrounding drug metabolism in fish, it is apparent that the capacity of fish liver to metabolize FLX in vitro is variable

THE SUBLETHAL DISRUPTING EFFECTS OF FLUOXETINE-HCI (FLX) ON CATALASE (CAT) ACTIVITY AND MALONDIALDEHYDE (MDA) LEVELS IN ZEBRAFISH, DANIO RERIO

The aim of this study is to investigate sub-lethal disrupting effects of fluoxetine-HCl (FLX) on catalase (CAT) activity and malondialdehyde (MDA) levels of liver tissue and whole body in zebrafish (Danio rerio). FLX is the active compound of the antidepressant Prozac(TM) and acts as a selective serotonin reuptake inhibitor (SSRI) in humans. Zebrafish are well-characterized model organism especially for the antioxidant enzyme activities for humans. Our study was planned as a model to investigate the effects of daily intake doses of FLX, which adapted from human proportionally to weight, on liver tissue of zebrafish. CAT, MDA and total protein levels were detected using spectrophotometric methods. The six experiment study groups were composed as; 150 ng fluoxetine-HCl exposed to each aquarium tank and five zebrafish were studied at 15 min., 30 min., 60 min., 4 days and 8 days of exposure and the last group was composed as the control group. In our study, it is determined that the activity of CAT increases in two experimental group after exposure FLX (15 min. and 30 min.) in liver tissue. Following one hour FLX treatment, it was observed that CAT activity decreased, whereas after four days treatment it was re-increased. Once for all, following 8 days treatment of FLX, it was observed that CAT activity significantly decreased. Likewise we found that the MDA levels decreases in all experimental groups after exposure FLX in liver tissue definitely. In whole body groups, MDA levels was firstly decreased after exposure FLX but MDA levels was increased subsequently compared to the control group in this study. In conclusion, building on the framework surrounding drug metabolism in fish, it is apparent that the capacity of fish liver to metabolize FLX in vitro is variable