7 research outputs found
Adherence of Streptococcus mutans to uncoated and saliva-coated glass-ceramics and composites.
Antimicrobial activity of Uncaria tomentosa against oral human pathogens
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Previous issue date: 2007Uncaria tomentosa is considered a medicinal plant used over centuries by the peruvian population as an alternative treatment for several diseases. Many microorganisms usually inhabit the human oral cavity and under certain conditions can become etiologic agents of diseases. The aim of the present study was to evaluate the antimicrobial activity of different concentrations of Uncaria tomentosa on different strains of microorganisms isolated from the human oral cavity. Micropulverized Uncaria tomentosa was tested in vitro to determine the minimum inhibitory concentration (MIC) on selected microbial strains. The tested strains were oral clinical isolates of Streptococcus mutans, Staphylococcus spp., Candida albicans, Enterobacteriaceae and Pseudomonas aeruginosa. The tested concentrations of Uncaria tomentosa ranged from 0.25-5% in Müeller-Hinton agat. Three percent Uncaria tomentosa inhibited 8% of Enterobacteriaceae isolates, 52% of S. mutans and 96% of Staphylococcus spp. The tested concentrations did not present inhibitory effect on P. aeruginosa and C. albicans. It could be concluded that micropulverized Uncaria tomentosa presented antimicrobial activity on Enterobacteriaceae, S. mutans and Staphylococcus spp. isolates.Ccahuana-Vasquez, R.A., Department of Physiology Sciences, School of Dentistry of Piracicaba, State University of Campinas, Campinas, BrazilFerreira dos Santos, S.S., Department of Microbiology, University of Taubaté, Rua dos Operários, 9, Taubaté, SP 12000-000, BrazilKoga-Ito, C.Y., Department of Bioscience and Oral Diagnosis, School of Dentistry of São José dos Campos, São Paulo State University, São Paulo, BrazilCardoso Jorge, A.O., Department of Microbiology, University of Taubaté, Rua dos Operários, 9, Taubaté, SP 12000-000, Brazi
Systematic Screening of Plant Extracts from the Brazilian Pantanal with Antimicrobial Activity against Bacteria with Cariogenic Relevance
Supplementary Material for: Systematic Screening of Plant Extracts from the Brazilian Pantanal with Antimicrobial Activity against Bacteria with Cariogenic Relevance
This study proposes a bioprospection methodology regarding the antimicrobial potential of plant extracts against bacteria with cariogenic relevance. Sixty extracts were obtained from ten plants - (1) <i>Jatropha weddelliana</i>, (2) <i>Attalea phalerata</i>, (3) <i>Buchenavia tomentosa</i>, (4) <i>Croton doctoris</i>, (5) <i>Mouriri elliptica</i>, (6) <i>Mascagnia benthamiana</i>, (7) <i>Senna aculeata</i>, (8) <i>Unonopsis guatterioides</i>, (9) <i>Allagoptera leucocalyx</i> and (10) <i>Bactris glaucescens</i> - using different extraction methods - (A) 70° ethanol 72 h/25°C, (B) water 5 min/100°C, (C) water 1 h/55°C, (D) water 72 h/25°C, (E) hexane 72 h/25°C and (F) 90° ethanol 72 h/25°C. The plants were screened for antibacterial activity at 50 mg/ml using the agar well diffusion test against <i>Actinomyces naeslundii</i> ATCC 19039, <i>Lactobacillus acidophilus</i> ATCC 4356, <i>Streptococcus gordonii</i> ATCC 10558, <i>Streptococcus mutans</i> ATCC 35688, <i>Streptococcus sanguinis</i> ATCC 10556, <i>Streptococcus sobrinus</i> ATCC 33478 and <i>Streptococcus mitis</i> ATCC 9811. The active extracts were tested to determine their minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), cytotoxicity and chemical characterization. Forty-seven extracts (78%) were active against at least one microorganism. Extract 4A demonstrated the lowest MIC and MBC for all microorganisms except <i>S. gordonii</i> and the extract at MIC concentration was non-cytotoxic. The concentrated extracts were slightly cytotoxic. Electrospray ionization with tandem mass spectrometry analyses demonstrated that the extract constituents coincided with the mass of the terpenoids and phenolics. Overall, the best results were obtained for extraction methods A, B and C. The present work proved the antimicrobial activity of several plants. Particularly, extracts from <i>C. doctoris</i> were the most active against bacteria involved in dental caries disease
Silver nanoparticles coated with dodecanethiol used as fillers in noncytotoxic and antifungal PBAT surface based on nanocomposites
In the present study, we report the preparation of antifungal and non-cytotoxic polymer nanocomposites with potential application in biomedical materials. Dodecanethiol-protected silver nanoparticles (AgNPs-DDT) were synthesized by a reduction/precipitation method and dispersed in chloroform to obtain stable colloidal dispersions. PBAT-based nanocomposites containing 0.25, 0.5 and 2 wt% AgNPs-DDT were prepared by casting method. The incorporation of AgNPs-DDT in PBAT matrix resulted in nanocomposites which combine improved mechanical performance and antifungal properties with a non-cytotoxic characteristic988008072016/09588-9; 2016/08595-1NÃO TEMNÃO TEMFAPESP – Fundação de Amparo à Pesquisa Do Estado De São PauloFAPESP – Fundação de Amparo à Pesquisa Do Estado De São PauloCAPES - Coordenação de Aperfeiçoamento de Pessoal e Nível SuperiorCNPQ - Conselho Nacional de Desenvolvimento Científico e Tecnológic