Presentation Evaluation Of Multistoried RC Particular Instant Resisting Structures

Strength analysis can be done in two ways, one is the response method and the other is the historical method. In response to the demo method, the letter was taken as the code for IS 1894 2003 but during the history the previous method was used for earthquake data. Loads conforming to me criteria are used. In this study, a time analysis report should be used because date analysis time is more expensive and feedback is the response method. Multi-storey buildings were read with (G + 10) articles using Tabs for Zone II Insulation in India. Using the historical time, the multi-storey building method calculated the transportation issue and the issue of borrowing. The analysis period is also known as nonlinear dynamic analysis. History of time is the highest form of energy analysis. The method of historical time analysis is also the ability to adjust the harmonic effect function that can be defined by sinusoidal curves having a specified arrival time, frequency, amplitude, and time

A Unique Presentation of Concomitant Hypo-Hyperdontia in Seven Year Old Child: A Rare Report

Numerical variations of teeth are common. Hypodontia considered being presence of less number of teeth in normal complement while extra teeth to normal dentition are considered as hyperdontia. Hypodontia and hyperdontia are two opposite numerical variations of human dentition and occurrence of these two conditions is called as concomitant hypo-hyperdontia. The occurrence of hypo-hyperdontia in a patient is common. This report describes a rare occurrence of conical shape supernumerary teeth in premolar region and agenesis of tooth 55 and 81 in primary dentition and teeth 15, 25 and 41 in permanent dentition.DOI: 10.14693/jdi.v22i3.97

Applicability of genetic algorithms to reconstruction of projected data from ultrasonic tomography

In this paper simulation studies of the ultrasound computerized tomography (CT) technique employing time of flight data is presented. An enhanced genetic algorithm based reconstruction technique is proposed that is capable of detecting multiple types of inclusions in the test specimen to be reconstructed. It is assumed that the physical properties of the inclusions are known a priori. The preliminary results of our algorithm for a simple configuration are found to be better than those reported with MART1. In addition to being able to identify inclusions of different materials, both the shape and location of the inclusions could be reconstructed using the proposed algorithm. The results are found to be consistent and satisfactory for a wide range of grid sizes and geometries of inclusion(s). Based on the regression analysis an empirical relation between the number of unknowns and the reconstruction time is found which enables one to predict the reconstruction time for higher resolutions

Dependence of recombination mechanisms and strength on processing conditions in polymer solar cells

Charge carrier recombination due to carrier trapping is not often considered in polymer based solar cells, except in those using non-fullerene acceptors or new donor polymers with limited short-range order. However, we show that even for the canonical poly(3-hexylthiophene): phenyl-C61-butyric acid methyl ester (P3HT:PCBM) system, relative strengths of bimolecular and trap-assisted recombination are strongly dependent on processing conditions. For slow-grown active-layers, bimolecular recombination is indeed the major loss mechanism under one sun illumination. However, for fast-grown active-layers, trap-assisted recombination dominates over bimolecular recombination by an order of magnitude, and recombination strength at short-circuit condition is 3-4 times higher, leading to loss of photocurrent and lowering of fill factor

Wwox deletion leads to reduced GABA-ergic inhibitory interneuron numbers and activation of microglia and astrocytes in mouse hippocampus

The association of WW domain-containing oxidoreductase WWOX gene loss of function with central nervous system (CNS) related pathologies is well documented. These include spinocerebellar ataxia, epilepsy and mental retardation (SCAR12, OMIM: 614322) and early infantile epileptic encephalopathy (EIEE28, OMIM: 616211) syndromes. However, there is complete lack of understanding of the pathophysiological mechanisms at play. In this study, using a Wwox knockout (Wwox KO) mouse model (2 weeks old, both sexes) and stereological studies we observe that Wwox deletion leads to a significant reduction in the number of hippocampal GABA-ergic (γ-aminobutyric acid) interneurons. Wwox KO mice displayed significantly reduced numbers of calcium-binding protein parvalbumin (PV) and neuropeptide Y (NPY) expressing interneurons in different subfields of the hippocampus in comparison to Wwox wild-type (WT) mice. We also detected decreased levels of Glutamic Acid Decarboxylase protein isoforms GAD65/67 expression in Wwox null hippocampi suggesting lower levels of GABA synthesis. In addition, Wwox deficiency was associated with signs of neuroinflammation such as evidence of activated microglia, astrogliosis, and overexpression of inflammatory cytokines Tnf-a and Il6. We also performed comparative transcriptome-wide expression analyses of neural stem cells grown as neurospheres from hippocampi of Wwox KO and WT mice thus identifying 283 genes significantly dysregulated in their expression. Functional annotation of transcriptome profiling differences identified ?neurological disease? and ?CNS development related functions? to be significantly enriched. Several epilepsy-related genes were found differentially expressed in Wwox KO neurospheres. This study provides the first genotype-phenotype observations as well as potential mechanistic clues associated with Wwox loss of function in the brain.Fil: Hussain, Tabish. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Kil, Hyunsuk. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Hattiangady, Bharathi. Texas A&M Health Science Center College of Medicine; Estados UnidosFil: Lee, Jaeho. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Kodali, Maheedhar. Texas A&M Health Science Center College of Medicine; Estados UnidosFil: Shuai, Bing. Texas A&M Health Science Center College of Medicine; Estados UnidosFil: Attaluri, Sahithi. Texas A&M Health Science Center College of Medicine; Estados UnidosFil: Takata, Yoko. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Shen, Jianjun. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados UnidosFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Shetty, Ashok K.. Texas A&M Health Science Center College of Medicine; Estados UnidosFil: Aldaz, Claudio Marcelo. University of Texas Health Science Center at Houston. University of Texas Md Anderson Cancer Center; Estados Unido

A Novel Disulfide-Rich Protein Motif from Avian Eggshell Membranes

Under the shell of a chicken egg are two opposed proteinaceous disulfide-rich membranes. They are fabricated in the avian oviduct using fibers formed from proteins that are extensively coupled by irreversible lysine-derived crosslinks. The intractability of these eggshell membranes (ESM) has slowed their characterization and their protein composition remains uncertain. In this work, reductive alkylation of ESM followed by proteolytic digestion led to the identification of a cysteine rich ESM protein (abbreviated CREMP) that was similar to spore coat protein SP75 from cellular slime molds. Analysis of the cysteine repeats in partial sequences of CREMP reveals runs of remarkably repetitive patterns. Module a contains a C-X4-C-X5-C-X8-C-X6 pattern (where X represents intervening non-cysteine residues). These inter-cysteine amino acid residues are also strikingly conserved. The evolutionarily-related module b has the same cysteine spacing as a, but has 11 amino acid residues at its C-terminus. Different stretches of CREMP sequences in chicken genomic DNA fragments show diverse repeat patterns: e.g. all a modules; an alternation of a-b modules; or an a-b-b arrangement. Comparable CREMP proteins are found in contigs of the zebra finch (Taeniopygia guttata) and in the oviparous green anole lizard (Anolis carolinensis). In all these cases the long runs of highly conserved modular repeats have evidently led to difficulties in the assembly of full length DNA sequences. Hence the number, and the amino acid lengths, of CREMP proteins are currently unknown. A 118 amino acid fragment (representing an a-b-a-b pattern) from a chicken oviduct EST library expressed in Escherichia coli is a well folded, highly anisotropic, protein with a large chemical shift dispersion in 2D solution NMR spectra. Structure is completely lost on reduction of the 8 disulfide bonds of this protein fragment. Finally, solid state NMR spectra suggest a surprising degree of order in intact ESM fibers

Analysis of Social Network Data Mining for Security Intelligence Privacy Machine Learning

The Modern communication on the Internet platform is most responsive through social media. Social media has changed and is still reshaping how we share our thoughts and emotions in communication. It has introduced a constant real-time communication pattern that was before unheard of. Young and old, organizations, governmental agencies, professional associations, etc., all have social media accounts that they use exclusively for communication with other users. Social media also acts as a powerful network engine that connects users regardless of where they are in the world. The development of global communication will greatly benefit from the availability of this new communication platform in the future. Consequently, there is a pressing need to research usage trends. Therefore, it is vital to investigate social media platform usage trends in order to develop automated systems that intelligence services can use to help avert national security incidents. Through the use of social media data mining, this research study suggests an automated machine learning model that can improve speedy response to crises involving national and International security

Identification of protein disulfide isomerase 1 as a key isomerase for disulfide bond formation in apolipoprotein B100

Apolipoprotein (apo) B is an obligatory component of very low density lipoprotein (VLDL), and its cotranslational and posttranslational modifications are important in VLDL synthesis, secretion, and hepatic lipid homeostasis. ApoB100 contains 25 cysteine residues and eight disulfide bonds. Although these disulfide bonds were suggested to be important in maintaining apoB100 function, neither the specific oxidoreductase involved nor the direct role of these disulfide bonds in apoB100-lipidation is known. Here we used RNA knockdown to evaluate both MTP-dependent and -independent roles of PDI1 in apoB100 synthesis and lipidation in McA-RH7777 cells. Pdi1 knockdown did not elicit any discernible detrimental effect under normal, unstressed conditions. However, it decreased apoB100 synthesis with attenuated MTP activity, delayed apoB100 oxidative folding, and reduced apoB100 lipidation, leading to defective VLDL secretion. The oxidative folding–impaired apoB100 was secreted mainly associated with LDL instead of VLDL particles from PDI1-deficient cells, a phenotype that was fully rescued by overexpression of wild-type but not a catalytically inactive PDI1 that fully restored MTP activity. Further, we demonstrate that PDI1 directly interacts with apoB100 via its redox-active CXXC motifs and assists in the oxidative folding of apoB100. Taken together, these findings reveal an unsuspected, yet key role for PDI1 in oxidative folding of apoB100 and VLDL assembly

Slow amyloid nucleation via α-helix-rich oligomeric intermediates in short polyglutamine-containing huntingtin fragments

The 17-amino-acid N-terminal segment (httNT) that leads into the polyglutamine (polyQ) segment in the Huntington\u27s disease protein huntingtin (htt) dramatically increases aggregation rates and changes the aggregation mechanism, compared to a simple polyQ peptide of similar length. With polyQ segments near or above the pathological repeat length threshold of about 37, aggregation of htt N-terminal fragments is so rapid that it is difficult to tease out mechanistic details. We describe here the use of very short polyQ repeat lengths in htt N-terminal fragments to slow this disease-associated aggregation. Although all of these peptides, in addition to httNT itself, form α-helix-rich oligomeric intermediates, only peptides with QN of eight or longer mature into amyloid-like aggregates, doing so by a slow increase in β-structure. Concentration-dependent circular dichroism and analytical ultracentrifugation suggest that the httNT sequence, with or without added glutamine residues, exists in solution as an equilibrium between disordered monomer and α-helical tetramer. Higher order, α-helix rich oligomers appear to be built up via these tetramers. However, only httNTQN peptides with N=8 or more undergo conversion into polyQ β-sheet aggregates. These final amyloid-like aggregates not only feature the expected high β-sheet content but also retain an element of solvent-exposed α-helix. The α-helix-rich oligomeric intermediates appear to be both on- and off-pathway, with some oligomers serving as the pool from within which nuclei emerge, while those that fail to undergo amyloid nucleation serve as a reservoir for release of monomers to support fibril elongation. Based on a regular pattern of multimers observed in analytical ultracentrifugation, and a concentration dependence of α-helix formation in CD spectroscopy, it is likely that these oligomers assemble via a four-helix assembly unit. PolyQ expansion in these peptides appears to enhance the rates of both oligomer formation and nucleation from within the oligomer population, by structural mechanisms that remain unclear. © 2011 Elsevier Ltd