Reduced contextually induced muscle thermogenesis in rats with calorie restriction and lower aerobic fitness but not monogenic obesity

We have previously identified predator odor as a potent stimulus activating thermogenesis in skeletal muscle in rats. As this may prove relevant for energy balance and weight loss, the current study investigated whether skeletal muscle thermogenesis was altered with negative energy balance, obesity propensity seen in association with low intrinsic aerobic fitness, and monogenic obesity. First, weight loss subsequent to three weeks of 50% calorie restriction suppressed the muscle thermogenic response to predator odor. Next, we compared rats bred based on artificial selection for intrinsic aerobic fitness—high- and low-capacity runners (HCR, LCR)—that display robust leanness and obesity propensity, respectively. Aerobically fit HCR showed enhanced predator odor-induced muscle thermogenesis relative to the less-fit LCR. This contrasted with the profound monogenic obesity displayed by rats homozygous for a loss of function mutation in Melanocortin 4 receptor (Mc4rK314X/K314X rats), which showed no discernable deficit in thermogenesis. Taken together, these data imply that body size or obesity per se are not associated with deficient muscle thermogenesis. Rather, the physiological phenotype associated with polygenic obesity propensity may encompass pleiotropic mechanisms in the thermogenic pathway. Adaptive thermogenesis associated with weight loss also likely alters muscle thermogenic mechanisms.</p

Pion-Production in Heavy-Ion Collisions at SIS energies

We investigate the production of pions in heavy-ion collisions in the energy range of $1$ - $2$ GeV/A. The dynamics of the nucleus-nucleus collisions is described by a set of coupled transport equations of the Boltzmann-Uehling-Uhlenbeck type for baryons and mesons. Besides the $N(938)$ and the $\Delta(1232)$ we also take into account nucleon resonances up to masses of $1.9 GeV/c^2$ as well as $\pi$-, $\eta$- and $\rho$-mesons. We study in detail the influence of the higher baryonic resonances and the $2\pi$-production channels ($NN\to NN \pi\pi$) on the pion spectra in comparison to $\pi^-$ data from $Ar + KCl$ collisions at $1.8$ GeV/A and $\pi^0$-data for $Au+Au$ at 1.0 GeV/A. We, furthermore, present a detailed comparison of differential pion angular distributions with the BEVALAC data for Ar + KCl at 1.8 GeV/A. The general agreement obtained indicates that the overall reactions dynamics is well described by our novel transport approach.Comment: 31 pages, 18 figures (inlcuded), to appear in Z. Phys.

Baryon Density Correlations in High Temperature Hadronic Matter

As part of an ongoing effort to characterize the high temperature phase of QCD, in a numerical simulation using the staggered fermion scheme, we measure the quark baryon density in the vicinity of a fixed test quark at high temperature and compare it with similar measurements at low temperature and at the crossover temperature. We find an extremely weak correlation at high temperature, suggesting that small color singlet clusters are unimportant in the thermal ensemble. We also find that at $T = 0.75\ T_c$ the total induced quark number shows a surprisingly large component attributable to baryonic screening. A companion simulation of a simple flux tube model produces similar results and also suggests a plausible phenomenological scenario: As the crossover temperature is approached from below, baryonic states proliferate. Above the crossover temperature the mean size of color singlet clusters grows explosively, resulting in an effective electrostatic deconfinement.Comment: 26 pp, RevTeX, 12 postscript figures, combined in a single shell archive file. (Also available in 13 postscript files by anonymous ftp from einstein.physics.utah.edu, /pub/milc/paper.sh.Z.

A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D2O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells were incubated in D2O-enriched media and myotubes ±50 ng/ml IGF-I. Second, rat quadriceps (untrained, n = 9; 7-wk interval-“like” training, n = 13) were collected after ~3-wk D2O (70 atom %) administration, with body-water enrichment monitored via blood sampling. Finally, 10 (23 ± 1 yr) men consumed 150-ml D2O followed by 50 ml/wk and undertook 6-wk resistance exercise (6 × 8 repetitions, 75% 1-repetition maximum 3/wk) with body-water enrichment monitored by saliva sampling and muscle biopsies (for determination of RNA synthesis) at 0, 3, and 6 wk. Ribose mole percent excess (r-MPE) from purine nucleotides was analyzed via GC-MS/MS. Proliferating C2C12 cell r-MPE exhibited a rise to plateau, whereas IGF-I increased myotube RNA from 76 ± 3 to 123 ± 3 ng/μl and r-MPE by 0.39 ± 0.1% (both P < 0.01). After 3 wk, rat quadriceps r-MPE had increased to 0.25 ± 0.01% (P < 0.01) and was greater with running exercise (0.36 ± 0.02%; P < 0.01). Human muscle r-MPE increased to 0.06 ± 0.01 and 0.13 ± 0.02% at 3/6 wk, respectively, equating to synthesis rates of ~0.8%/day, increasing with resistance exercise to 1.7 ± 0.3%/day (P < 0.01) and 1.2 ± 0.1%/day (P < 0.05) at 3/6 wk, respectively. Therefore, we have developed and physiologically validated a novel technique to explore ribosomal biogenesis in a multimodal fashion

A versatile method for simulating pp -> ppe+e- and dp -> pne+e-p_spec reactions

We have developed a versatile software package for the simulation of di-electron production in $pp$ and $dp$ collisions at SIS energies. Particular attention has been paid to incorporate different descriptions of the Dalitz decay $\Delta \to N e^+e^-$ via a common interface. In addition, suitable parameterizations for the virtual bremsstrahlung process $NN \to NN e^+e^-$ based on one-boson exchange models have been implemented. Such simulation tools with high flexibility of the framework are important for the interpretation of the di-electron data taken with the HADES spectrometer and the design of forthcoming experiments

Dilepton Spectra from Decays of Light Unflavored Mesons

The invariant mass spectrum of the $e^{+}e^{-}$ and $\mu ^{+}\mu ^{-}$ pairs from decays of light unflavored mesons with masses below the $\phi (1020)$-meson mass to final states containing along with a dilepton pair one photon, one meson, and two mesons are calculated within the framework of the effective meson theory. The results can be used for simulations of the dilepton spectra in heavy-ion collisions and for experimental searches of dilepton meson decays.Comment: 73 pages, 19 figures, 3 tables, REVTeX, new references adde

Hydrodynamical Description of 200 A GeV/c S+Au Collisions: Hadron and Electromagnetic Spectra

We study relativistic S+Au collisions at 200 A GeV/c using a hydrodynamical approach. We test various equations of state (EOSs), which are used to describe the strongly interacting matter at densities attainable in the CERN-SPS heavy ion experiments. For each EOS, suitable initial conditions can be determined to reproduce the experimental hadron spectra; this emphasizes the ambiguity between the initial conditions and the EOS in such an approach. Simultaneously, we calculate the resulting thermal photon and dielectron spectra, and compare with experiments. If one allows the excitation of resonance states with increasing temperature, the electro-magnetic signals from scenarios with and without phase transition are very similar and are not resolvable within the current experimental resolution. With regard to the CERES dilepton data, none of the EOSs considered, in conjunction with the standard leading order dilepton rates, succeed in reproducing the observed excess of dileptons below the rho peak. Our work, however, suggests that an improved measurement of the photon and dilepton spectra has the potential to strongly constrain the EOS.Comment: Uses REVTeX, 48 pages, 13 Postscript figure

Measurement of the Proton Spin Structure Function g1p with a Pure Hydrogen Target

A measurement of the proton spin structure function g1p(x,Q^2) in deep-inelastic scattering is presented. The data were taken with the 27.6 GeV longitudinally polarised positron beam at HERA incident on a longitudinally polarised pure hydrogen gas target internal to the storage ring. The kinematic range is 0.021<x<0.85 and 0.8 GeV^2<Q^2<20 GeV^2. The integral Int_{0.021}^{0.85} g1p(x)dx evaluated at Q0^2 of 2.5 GeV^2 is 0.122+/-0.003(stat.)+/-0.010(syst.).Comment: 7 pages, 3 figures, 1 table, RevTeX late

Determination of the Deep Inelastic Contribution to the Generalised Gerasimov-Drell-Hearn Integral for the Proton and Neutron

The virtual photon absorption cross section differences [sigma_1/2-sigma_3/2] for the proton and neutron have been determined from measurements of polarised cross section asymmetries in deep inelastic scattering of 27.5 GeV longitudinally polarised positrons from polarised 1H and 3He internal gas targets. The data were collected in the region above the nucleon resonances in the kinematic range nu < 23.5 GeV and 0.8 GeV**2 < Q**2 < 12 GeV**2. For the proton the contribution to the generalised Gerasimov-Drell-Hearn integral was found to be substantial and must be included for an accurate determination of the full integral. Furthermore the data are consistent with a QCD next-to-leading order fit based on previous deep inelastic scattering data. Therefore higher twist effects do not appear significant.Comment: 6 pages, 3 figures, 1 table, revte

Observation of a Coherence Length Effect in Exclusive Rho^0 Electroproduction

Exclusive incoherent electroproduction of the rho^0(770) meson from 1H, 2H, 3He, and 14N targets has been studied by the HERMES experiment at squared four-momentum transfer Q**2>0.4 GeV**2 and positron energy loss nu from 9 to 20 GeV. The ratio of the 14N to 1H cross sections per nucleon, known as the nuclear transparency, was found to decrease with increasing coherence length of quark-antiquark fluctuations of the virtual photon. The data provide clear evidence of the interaction of the quark- antiquark fluctuations with the nuclear medium.Comment: RevTeX, 5 pages, 3 figure