Infekcja dakronowej protezy pachowo-dwuudowej wykonanej z powodu niedrożności aorty brzusznej - indywidualna, dostosowana do przypadku taktyka postępowania

An infection of an axillobifemoral prosthetic graft was treated by partially in situ reconstruction using autologous vein and artery. Autologous material for a new axillofemoral graft was obtained using the superficial femoral vein and the thrombendarterectomised superficial femoral artery of the same extremity. Chronic venous insufficiency did not result. Recurrent stenosis of the arterial autograft required a redo-thrombendarterectomy with patch plasty 17 months postoperatively.Zakażenie protezy przeszczepu pachowo-dwuudowego leczono, wykonując rekonstrukcję z wykorzystaniem autologicznej żyły i tętnicy. Materiał autologiczny do nowego przeszczepu pachowo-dwuudowego uzyskano z żyły udowej powierzchownej oraz z tętnicy udowej powierzchownej, uprzednio poddanej trombendarterektomii; pochodzących z jednej kończyny. Nie obserwowano cech przewlekłej niewydolności żylnej. Nawrót zwężenia autologicznego przeszczepu tętniczego wystąpił po 17 miesiącach od zabiegu i wymagał powtórnej trombendarterektomii oraz plastyki z użyciem łaty

Increased Terpenoid Accumulation in Cotton (Gossypium hirsutum) Foliage is a General Wound Response

The subepidermal pigment glands of cotton accumulate a variety of terpenoid products, including monoterpenes, sesquiterpenes, and terpenoid aldehydes that can act as feeding deterrents against a number of insect herbivore species. We compared the effect of herbivory by Spodoptera littoralis caterpillars, mechanical damage by a fabric pattern wheel, and the application of jasmonic acid on levels of the major representatives of the three structural classes of terpenoids in the leaf foliage of 4-week-old Gossypium hirsutum plants. Terpenoid levels increased successively from control to mechanical damage, herbivory, and jasmonic acid treatments, with E-β-ocimene and heliocide H1 and H4 showing the highest increases, up to 15-fold. Herbivory or mechanical damage to older leaves led to terpenoid increases in younger leaves. Leaf-by-leaf analysis of terpenes and gland density revealed that higher levels of terpenoids were achieved by two mechanisms: (1) increased filling of existing glands with terpenoids and (2) the production of additional glands, which were found to be dependent on damage intensity. As the relative response of individual terpenoids did not differ substantially among herbivore, mechanical damage, and jasmonic acid treatments, the induction of terpenoids in cotton foliage appears to represent a non-specific wound response mediated by jasmonic acid

Influence of Narratives of Vision and Identity on Collective Behavior Change

Profound societal transformations are needed to move society from unsustainability to greater sustainability under continually changing social and environmental conditions. A key challenge is to understand the influences on and the dynamics of collective behavior change toward sustainability. In this paper we describe our approach to (1) understanding how affective narrative expressions influence transitions to more sustainable collective behaviors and (2) how that understanding, as well as the potential for using narrative expressions in modeling of social movements, can become a basis for improving community responses to change in a rapidly changing world. Our focus is on narratives that express visions of desirable futures and narratives that reflect individual and social identities, on the cultures and contexts in which they are embedded, exchanged, and modified, and through which they influence the dynamics of social movements toward sustainability. Using an analytical categorization of narrative expressions of case studies in the Caribbean, Micronesia, and Africa, we describe insights derived from the narratives of vision and social identities in diverse communities. Finally, we suggest that narrative expressions may provide a basis for agent-based modeling to expand thinking about potential development pathways of social movements for sustainable futures

Contactless in ovo sex determination of chicken eggs

We resume the research for contactless in ovo sex determination of the domestic chicken, which has the final aim to provide an alternative to day-old cockerels culling in the layer industry. In ovo Raman and fluorescence spectroscopy of blood of eggs incubated until day 3.5 enables correct sexing rates over 90% barely affecting the hatching rate. Full automatization of the processes to guarantee high sexing speed and fulfill industrial demands is required to allow transferring the technology inside the hatcheries in the next future

Highly sensitive and quick in ovo sexing of domestic chicken eggs by two-wavelength fluorescence spectroscopy

The in ovo sexing of chicken eggs is a current task and a prerequisite to overcome the mass killing of male day-old chicks from laying lines. Although various methods have been developed and tested in recent years, practicable methods for sex determination are still missing which can be applicated in poultry hatcheries before the chicken embryo is capable of nociception and pain sensation. Optical spectroscopic methods enable an early determination of the sex. In this study, a novel method based on two-wavelength in ovo fluorescence excitation is described. More than 1600 eggs were examined. In ovo fluorescence was sequentially excited at 532 nm and 785 nm. The fluorescence intensities of the spectral regions behave inversely with respect to sex. It is shown that the observed sex-related differences in the fluorescence intensities are based on the embryonic hemoglobin synthesis. The accuracy of sex determination is 96% for both sexes. The hatching rate is not reduced compared to an equivalent reference group

Screening for Protein-DNA Interactions by Automatable DNA-Protein Interaction ELISA

DNA-binding proteins (DBPs), such as transcription factors, constitute about 10% of the protein-coding genes in eukaryotic genomes and play pivotal roles in the regulation of chromatin structure and gene expression by binding to short stretches of DNA. Despite their number and importance, only for a minor portion of DBPs the binding sequence had been disclosed. Methods that allow the de novo identification of DNA-binding motifs of known DBPs, such as protein binding microarray technology or SELEX, are not yet suited for high-throughput and automation. To close this gap, we report an automatable DNA-protein-interaction (DPI)-ELISA screen of an optimized double-stranded DNA (dsDNA) probe library that allows the high-throughput identification of hexanucleotide DNA-binding motifs. In contrast to other methods, this DPI-ELISA screen can be performed manually or with standard laboratory automation. Furthermore, output evaluation does not require extensive computational analysis to derive a binding consensus. We could show that the DPI-ELISA screen disclosed the full spectrum of binding preferences for a given DBP. As an example, AtWRKY11 was used to demonstrate that the automated DPI-ELISA screen revealed the entire range of in vitro binding preferences. In addition, protein extracts of AtbZIP63 and the DNA-binding domain of AtWRKY33 were analyzed, which led to a refinement of their known DNA-binding consensi. Finally, we performed a DPI-ELISA screen to disclose the DNA-binding consensus of a yet uncharacterized putative DBP, AtTIFY1. A palindromic TGATCA-consensus was uncovered and we could show that the GATC-core is compulsory for AtTIFY1 binding. This specific interaction between AtTIFY1 and its DNA-binding motif was confirmed by in vivo plant one-hybrid assays in protoplasts. Thus, the value and applicability of the DPI-ELISA screen for de novo binding site identification of DBPs, also under automatized conditions, is a promising approach for a deeper understanding of gene regulation in any organism of choice

Sexing of chicken eggs by fluorescence and Raman spectroscopy through the shell membrane

<div><p>In order to provide an alternative to day-old chick culling in the layer hatcheries, a noninvasive method for egg sexing is required at an early stage of incubation before onset of embryo sensitivity. Fluorescence and Raman spectroscopy of blood offers the potential for precise and contactless in ovo sex determination of the domestic chicken (<i>Gallus gallus</i> f. dom.) eggs already during the fourth incubation day. However, such kind of optical spectroscopy requires a window in the egg shell, is thus invasive to the embryo and leads to decreased hatching rates. Here, we show that near infrared Raman and fluorescence spectroscopy can be performed on perfused extraembryonic vessels while leaving the inner egg shell membrane intact. Sparing the shell membrane makes the measurement minimally invasive, so that the sexing procedure does not affect hatching rates. We analyze the effect of the membrane above the vessels on fluorescence signal intensity and on Raman spectrum of blood, and propose a correction method to compensate for it. After compensation, we attain a correct sexing rate above 90% by applying supervised classification of spectra. Therefore, this approach offers the best premises towards practical deployment in the hatcheries.</p></div