Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novel ornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl(-1) with NAA ( 0.0, 0.5, 1.0 mgl(-1)) and BA (0.0, 0.5, 1.0, 2.0 mgl(-1)) with IAA ( 0.0, 0.5, 1.0, mgl(-1)) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl(-1) BA and 1.0 mgl(-1) NAA (70%) followed by 1.0 mgl(-1) BA and 0.5 mgl(-1) NAA (50%), 1.0 mgl(-1) BA and 1.0 mgl(-1) NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl(-1) BA and 0.5 mgl(-1) IAA (43%) followed by 0.5 mgl(-1) BA and 1.0 mgl(-1) IAA (23%)

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novelornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-¹ with NAA (0.0, 0.5, 1.0 mgl-¹) and BA (0.0, 0.5, 1.0, 2.0 mgl-¹) with IAA (0.0, 0.5, 1.0, mgl-¹) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-¹ BA and 1.0 mgl-¹ NAA (70%) followed by 1.0 mgl-¹ BA and 0.5 mgl-¹ NAA (50%), 1.0 mgl-¹ BA and 1.0 mgl-¹ NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-¹ BA and 0.5 mgl-¹ IAA (43%) followed by 0.5 mgl-¹ BA and 1.0 mgl-¹ IAA (23%)

MULTI-HAZARD SUSCEPTIBILITY ASSESSMENT WITH HYBRID MACHINE LEARNING METHODS FOR TUT REGION (ADIYAMAN, TURKIYE)

Recent Kahramanmaras earthquakes (Mw 7.7 and 7.6) occurred on 6 February 2023 have shown the importance of site selection for settlements and infrastructure considering the fact that multiple hazards may affect the same area and even interact with each other. The Kahramanmaras earthquakes triggered several landslides, which also increased the level of destruction. Here, we implemented a multi-hazard susceptibility assessment approach for Tut town of Golbasi, Adiyaman and its surroundings. Over 600 landslides were triggered in the area by the earthquakes. In addition, the region is prone to flooding and a devastating one occurred on March 15, 2023 after heavy rains. In this study, we employed co-seismic landslide inventory for landslide susceptibility assessment with random forest. Regarding flood susceptibility, a modified analytical hierarchical process was utilized based on expert opinion on factor importance. The earthquake hazard probability distribution was obtained from a distance-based interpolation of Arias intensity values. We utilized Mamdani Fuzzy Inference System for producing a multi-hazard susceptibility map from univariate maps of earthquake, landslide and flood. The result shows that the selected methods for each type of susceptibility map was suitable and the output of the study can be utilized for the site selection in Tut region, which is a crucial subject due to the need of new construction sites after the earthquakes

FREQUENCY RATIO ASSESSMENT FOR LANDSLIDES TRIGGERED BY 6 FEBRUARY 2023 KAHRAMANMARAS TURKIYE EARTHQUAKES BETWEEN GOLBASI AND ERKENEK

Landslides triggered by earthquakes are significant geological hazards that can have devastating consequences, posing risks to human lives, infrastructure, and the environment. These seismic events may cause the instability of slopes and result in the displacement of soil and rock materials, leading to landslides. It is crucial to understand the characteristics and mechanisms of earthquake-triggered landslides in order to effectively manage and mitigate their associated risks. The number of landslides triggered by the 2023 Kahramanmaraş earthquakes (with magnitudes of 7.7 and 7.6) was over three thousand and their destructive effects were also devastating as secondary hazards. This study aims to examine the characteristics of landslides using the frequency ratio (FR) model. A landslide susceptibility map (LSM) was also produced using the output. For this purpose, in this study, we derived landslides triggered by the earthquakes in a part of the earthquake-affected region, between Golbasi town of Adiyaman and Erkenek village of Malatya covering an area with a size of 625 km2. The study utilized a landslide inventory that was manually delineated by visual interpretation based on pre-event and post-event. These associations can serve as a foundation for the application of various data-driven machine learning techniques. The findings of this study will contribute to the development of accurate LSMs, providing crucial insights into the behavior of earthquake-triggered landslides

Prognostic value of fractional flow reserve: Linking physiologic severity to clinical outcomes

BACKGROUND: Fractional flow reserve (FFR) has become an established tool for guiding treatment, but its graded relationship to clinical outcomes as modulated by medical therapy versus revascularization remains unclear.OBJECTIVES: The study hypothesized that FFR displays a continuous relationship between its numeric value and prognosis, such that lower FFR values confer a higher risk and therefore receive larger absolute benefits from revascularization.METHODS: Meta-analysis of study- and patient-level data investigated prognosis after FFR measurement. An interaction term between FFR and revascularization status allowed for an outcomes-based threshold.RESULTS: A total of 9,173 (study-level) and 6,961 (patient-level) lesions were included with a median follow-up of 16 and 14 months, respectively. Clinical events increased as FFR decreased, and revascularization showed larger net benefit for lower baseline FFR values. Outcomes-derived FFR thresholds generally occurred around the range 0.75 to 0.80, although limited due to confounding by indication. FFR measured immediately after stenting also showed an inverse relationship with prognosis (hazard ratio: 0.86, 95% confidence interval: 0.80 to 0.93; p < 0.001). An FFR-assisted strategy led to revascularization roughly half as often as an anatomy-based strategy, but with 20% fewer adverse events and 10% better angina relief.CONCLUSIONS: FFR demonstrates a continuous and independent relationship with subsequent outcomes, modulated by medical therapy versus revascularization. Lesions with lower FFR values receive larger absolute benefits from revascularization. Measurement of FFR immediately after stenting also shows an inverse gradient of risk, likely from residual diffuse disease. An FFR-guided revascularization strategy significantly reduces events and increases freedom from angina with fewer procedures than an anatomy-based strategy

Assessment of imidacloprid toxicity on reproductive organ system of adult male rats

In the current study it was aimed to investigate the toxicity of low doses of imidacloprid (IMI) on the reproductive organ systems of adult male rats. The treatment groups received 0.5 (IMI-0.5), 2 (IMI-2) or 8 mg IMI/kg body weight by oral gavage (IMI-8) for three months. The deterioration in sperm motility in IMI-8 group and epidydimal sperm concentration in IMI-2 and IMI-8 groups and abnormality in sperm morphology in IMI-8 were significant. The levels of testosterone (T) and GSH decreased significantly in group IMI-8 compared to the control group. Upon treatment with IMI, apoptotic index increased significantly only in germ cells of the seminiferous tubules of IMI-8 group when compared to control. Fragmentation was striking in the seminal DNA from the IMI-8 group, but it was much less obvious in the IMI-2 one. IMI exposure resulted in elevation of all fatty acids analyzed, but the increases were significant only in stearic, oleic, linoleic and arachidonic acids. The ratios of 20:4/20:3 and 20:4/18:2 were decreased and 16:1n-9/16:0 ratio was increased. In conclusion, the present animal experiments revealed that the treatment with IMI at NOAEL dose-levels caused deterioration in sperm parameters, decreased T level, increased apoptosis of germ cells, seminal DNA fragmentation, the depletion of antioxidants and change in disturbance of fatty acid composition. All these changes indicate the suppression of testicular function

Concurrent validity of self-rating scale of self-directed learning and self-directed learning instrument among Italian nursing students

BACKGROUND: Self-Directed Learning develops when students take the initiative for their learning, recognising needs, formulating goals, identifying resources, implementing appropriate strategies and evaluating learning outcomes. This should be seen as a collaborative process between the nurse educator and the learner. At the international level, various instruments have been used to measure Self-Directed Learning abilities (SDL), both in original and in culturally-adapted versions. However, few instruments have been subjected to full validation, and no gold standard reference has been established to date. In addition, few researchers have adopted the established tools to assess the concurrent validity of the emerging new tools. Therefore, the aim of this study was to measure the concurrent validity between the Self-Rating Scale of Self-Directed Learning (SRSSDL_Ita) - Italian version and the Self-Directed Learning Instruments (SDLI) in undergraduate nursing students. METHODS: A concurrent validity study design was conducted in a Bachelor level nursing degree programme located in Italy. All nursing students attending the first, second or third year (n=428) were the target sample. The SRSSDL_Ita, and the SDLI were used. The Pearson correlation was used to determine the concurrent validity between the instruments; the confidence of intervals (CI 95%) bias-corrected and accelerated bootstrap (BCa), were also calculated. RESULTS: The majority of participants were students attending their first year (47.9%), and were predominately female (78.5%). Their average age was 22.5\ub14.1. The SDL abilities scores, as measured with the SRSSDL_Ita (min 40, max 200), were, on average, 160.79 (95% CI 159.10-162.57; median 160); while with the SDLI (min 20, max 100), they were on average 82.57 (95% CI 81.79-83.38; median 83). The Pearson correlation between the SRSSDL_Ita and SDLI instruments was 0.815 (CI BCa 95% 0.774-0.848), (p=0.000). CONCLUSIONS: The findings confirm the concurrent validity of the SRSSDL_Ita with the SDLI. The SRSSDL_Ita instrument can be useful in the process of identifying Self-Directed Learning abilities, which are essential for students to achieve the expected learning goals and become lifelong learners

Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics.

Nsp15, a uridine specific endoribonuclease conserved across coronaviruses, processes viral RNA to evade detection by host defense systems. Crystal structures of Nsp15 from different coronaviruses have shown a common hexameric assembly, yet how the enzyme recognizes and processes RNA remains poorly understood. Here we report a series of cryo-EM reconstructions of SARS-CoV-2 Nsp15, in both apo and UTP-bound states. The cryo-EM reconstructions, combined with biochemistry, mass spectrometry, and molecular dynamics, expose molecular details of how critical active site residues recognize uridine and facilitate catalysis of the phosphodiester bond. Mass spectrometry revealed the accumulation of cyclic phosphate cleavage products, while analysis of the apo and UTP-bound datasets revealed conformational dynamics not observed by crystal structures that are likely important to facilitate substrate recognition and regulate nuclease activity. Collectively, these findings advance understanding of how Nsp15 processes viral RNA and provide a structural framework for the development of new therapeutics