Impact of Maintenance on Domestic Wastewater Treatment Systems

Wastewater treatment systems are important sources of contaminants of emerging substances, including pharmaceuticals, and personal care products. Onsite wastewater treatment systems provide alternative solutions to centralized systems; although they are becoming increasingly popular, little is known about the effect of maintenance on their performance. In the current study, chemical and microbiological parameters in the effluents from two identical on-site wastewater treatment systems were analyzed, one being properly maintained while the other not maintained at all. Taxonomic profiles vastly differed from each other, and organic micropollutants are present at higher concentrations in the effluent of the non-maintained unit. The results highlight the importance of proper maintenance

The Justy mutation identifies Gon4-like as a gene that is essential for B lymphopoiesis

A recessive mutation named Justy was found that abolishes B lymphopoiesis but does not impair other major aspects of hematopoiesis. Transplantation experiments showed that homozygosity for Justy prevented hematopoietic progenitors from generating B cells but did not affect the ability of bone marrow stroma to support B lymphopoiesis. In bone marrow from mutant mice, common lymphoid progenitors and pre-pro–B cells appeared normal, but cells at subsequent stages of B lymphopoiesis were dramatically reduced in number. Under culture conditions that promoted B lymphopoiesis, mutant pre-pro–B cells remained alive and began expressing the B cell marker CD19 but failed to proliferate. In contrast, these cells were able to generate myeloid or T/NK precursors. Genetic and molecular analysis demonstrated that Justy is a point mutation within the Gon4-like (Gon4l) gene, which encodes a protein with homology to transcriptional regulators. This mutation was found to disrupt Gon4l pre-mRNA splicing and dramatically reduce expression of wild-type Gon4l RNA and protein. Consistent with a role for Gon4l in transcriptional regulation, the levels of RNA encoding C/EBPα and PU.1 were abnormally high in mutant B cell progenitors. Our findings indicate that the Gon4l protein is required for B lymphopoiesis and may function to regulate gene expression during this process

Prediction of long-term localized corrosion rates in a carbon steel cooling water system is enhanced by metagenome analysis

To predict variation of maximum localized penetration with exposure time, long-term localized corrosion was assessed in an emergency cooling water system composed of two carbon steel pipelines of 700 mm diameter transporting raw river water at flow velocities of 1 m/s and 0.1 m/s. Field tests, visual inspection, ultrasonic testing, BART testing, SEM-EDS and metagenomic analyses were performed to assess the progress of long-term corrosion and determine the influence of microbes in the corrosion process. High corrosion was linked to sulphate reducing bacteria and potentially to methanogenic archaea in the low-velocity pipeline, while moderate corrosion was linked to non-sulphate reducing bacteria in the higher velocity pipeline. Using historical and literature data available as well as our own test results, an empirical model was developed to predict Maximum Localized Penetration change over time to be applied in the ageing management of cooling water systems. Molecular Microbiological Methods in combination with traditional techniques are useful tools in the ageing management of pipelines. By applying the empirical model developed and the approach presented, unexpected through-wall leaking can be avoided, thus, saving costs and assets

Genome-level insights into the operation of an on-site biological wastewater treatment unit reveal the importance of storage time

On-site wastewater treatment systems are gaining popularity in areas where centralized wastewater treatment is not available. In the current case study a domestic activated sludge system was investigated, where treated effluent was stored in a short-term (1 week turn-over time) and a long-term (over 2–3 months) storage tank and was then used for irrigation. This design provided a unique opportunity to assess the chemical and microbial changes of the effluent upon storage. Long-term storage greatly improved both the chemical quality and the degradation efficiency of most organic micropollutants examined, including petroleum hydrocarbons and the pesticide diethyltoluamide. Taxonomic profile of the core microbiome of the effluent was also influenced upon storage. Relative abundance values of the members of Azoarcus and Thauera genera, which are important in degrading polycyclic aromatic hydrocarbons compounds, clearly indicated the biodegrading activity of these microbes across samples. The abundance of xenobiotics degradation functions correlated with the observed organic micropollutant degradation values indicating efficient microbial decomposition of these contaminants. Functions related to infectious diseases also had the highest abundance in the short-term storage tank corresponding well with the relative abundance of indicator organisms and implying to the significance of storage time in the elimination of pathogens. Based on these results, small, on-site wastewater treatment systems could benefit from long-term storage of wastewater effluent

Mechanism of Ca2+ activation of the NADPH oxidase 5 (NOX5)

NADPH oxidase 5 (NOX5) is a homologue of the gp91(phox) subunit of the phagocyte NADPH oxidase. NOX5 is expressed in lymphoid organs and testis and distinguished from the other NADPH oxidases by its unique N terminus, which contains three canonical EF-hands, Ca(2+)-binding domains. Upon heterologous expression, NOX5 was shown to generate superoxide in response to intracellular Ca(2+) elevations. In this study, we have analyzed the mechanism of Ca(2+) activation of NOX5. In a cell-free system, Ca(2+) elevations triggered superoxide production by NOX5 (K(m) = 1.06 microm) in an NADPH- and FAD-dependent but cytosol-independent manner. That result indicated a role for the N-terminal EF-hands in NOX5 activation. Therefore, we generated recombinant proteins of NOX5 N terminus and investigated their interactions with Ca(2+). Flow dialysis experiments showed that NOX5 N terminus contained four Ca(2+)-binding sites and allowed us to define the hitherto unidentified fourth, non-canonical EF-hand. The EF-hands of NOX5 formed two pairs: the very N-terminal pair had relatively low affinity for Ca(2+), whereas the more C-terminal pair bound Ca(2+) with high affinity. Ca(2+) binding caused a marked conformation change in the N terminus, which exposed its hydrophobic core, and became able to bind melittin, a model peptide for calmodulin targets. Using a pull-down assay, we demonstrate that the regulatory N terminus and the catalytic C terminus of NOX5 interact in a Ca(2+)-dependent way. Our results indicate that the Ca(2+)-induced conformation change of NOX5 N terminus led to enzyme activation through an intra-molecular interaction. That represents a novel mechanism of activation among NAD(P)H oxidases and Ca(2+)-activated enzymes

Long-Term Changes of Species Composition and Functional Traits of Epiphytic Diatoms in the Szigetköz Region (Hungary) of the Danube River

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