3 research outputs found
Liver Fibrosis and Metabolic Alterations in Adults With alpha-1-antitrypsin Deficiency Caused by the Pi*ZZ Mutation
BACKGROUND & AIMS: Alpha-1 antitrypsin deficiency (AATD) is among the most common genetic disorders. Severe AATD is caused by a homozygous mutation in the SERPINA1 gene that encodes the Glu342Lys substitution (called the Pi*Z mutation,
Pi*ZZ genotype). Pi*ZZ carriers may develop lung and liver diseases. Mutation- associated lung disorders have been well studied, but less is known about the effects in liver. We assessed the liver disease burden and associated features in
adults with this form of AATD. METHODS: We collected data from 554 Pi*ZZ adults (403 in an exploratory cohort, 151 in a confirmatory cohort), in 9 European countries, with AATD who were homozygous for the Pi*Z mutation, and 234 adults without
the Pi*Z mutation (controls), all without pre-existing liver disease. We collected data on demographic parameters, comorbidities, lung- and liver-related health, and blood samples for laboratory analysis. Liver fibrosis was assessed non-invasively
via the serum tests Aspartate Aminotransferase to Platelet Ratio Index and HepaScore and via transient elastography. Liver steatosis was determined via transient elastography-based controlled attenuation parameter. We performed histologic
analyses of livers from transgenic mice that overexpress the AATD-associated Pi*Z variant. RESULTS: Serum levels of liver enzymes were significantly higher in Pi*ZZ carriers vs controls. Based on non-invasive tests for liver fibrosis, significant fibrosis
was suspected in 20%–36% of Pi*ZZ carriers, whereas signs of advanced fibrosis were 9- to 20-fold more common in Pi*ZZ carriers compared to non-carriers. Male sex; age older than 50 years; increased levels of alanine aminotransferase, aspartate
aminotransferase, or g-glutamyl transferase; and low numbers of platelets were associated with higher liver fibrosis burden. We did not find evidence for a relationship between lung function and liver fibrosis. Controlled attenuation parameter 280 dB/m, suggesting severe steatosis, was detected in 39% of Pi*ZZ carriers vs 31% of controls. Carriers of Pi*ZZ had lower serum concentrations of triglyceride and low- and very-lowdensity lipoprotein cholesterol than controls, suggesting impaired hepatic secretion of lipid. Livers from Pi*Zoverexpressing mice had steatosis and down-regulation of
genes involved in lipid secretion. CONCLUSIONS: In studies of AATD adults with the Pi*ZZ mutation, and of Pi*Z-overexpressing mice, we found evidence of liver steatosisinfo:eu-repo/semantics/publishedVersio
O sistema tradicional de exploração de ovinos em Bragança
Este número da série Estudos reproduz parte significativa de uma tese de Mestrado apresentada na UTAD - Universidade de Trás-os-Montes e Alto Douro, em 1993, com o tÃtulo Pastores, rebanhos de ovinos e pastoreio. Que futuro para o sistema tradicional de exploração.
Da tese original, retirámos um capÃtulo, referente ao estudo da produção ovina numa aldeia do concelho de Bragança e ao estudo económico das explorações de ovinos dessa aldeia; e alguns anexos, cuja ausência não prejudicam a leitura e compreensão deste texto.
Passados estes anos, o texto perdeu alguma actualidade, pois algumas das condições então analisadas sofreram alterações. Porque conhecemos a evolução entretanto verificada (o nosso trabalho de investigação, realizado entretanto, permitiu acompanhar a actividade) podemos afirmar que o texto não perdeu, nos seus aspectos fundamentais, actualidade. Podemos referir que as alterações mais importantes que se verificaram se relacionam com aspectos de legislação (por exemplo, regulamentação da actividade, posturas autárquicas); com as modificações verificadas nalguns organismos ou instituições (por exemplo, serviços ligados ao Ministério da Agricultura); alterações nas normas e regulamentos de apoio e subsÃdios concedidos aos criadores de ovinos.
Será interessante, no futuro, fazer a comparação, relativamente a este estudo, e analisar a evolução do sistema tradicional de exploração de ovinos, e procurar encontrar as razões das alterações verificadas