Sutton Hoo: the body in the mound

Seven miles from the Deben River in Suffolk, England, is a large pagan cemetery named Sutton Hoo, which consists of seventeen burial mounds. The most impressive of these mounds contains a ninety-foot Anglo-Saxon ship buried beneath the earth. Atop the ship is a burial chamber that contained artifacts such as: a helmet, sword, shield, scepter, standard and a purse holding thirty-seven Merovingian coins. This ship-burial has intrigued scholars since it was discovered and subsequently excavated in 1939. Dozens of theories still circulate on the burial’s intended purpose and date as well as whether or not there was an individual buried within, and if so, who. This thesis will discuss the royal artifacts found inside the burial chamber of the ship and conclude, based on historical writings and physical evidence, that a body was interred and will identify the deceased. By regarding the artifacts as regalia, objects associated with kingship, it can be established that the grave is that of a supreme ruler. The issue of who is venerated by the ship-burial can best be determined by the proper dating of the burial itself. The dates are largely dictated by the coins and have changed several times in the sixty years since their discovery. The year of c. 625 A.D. was finally agreed upon by experts at the British museum. The vacillation in the dating of the coins has led to various hypotheses as to who was memorialized by the elaborate ship-burial and why. In this paper, the various theories as to the occupant of the mound will be addressed and scrutinized. Based on the evidence presented, I will conclude that the burial did contain a body and it is that of the seventh-century king of East Anglia, Rædwald, who died in c. 625. The artifacts included in the ship-burial are some of the finest examples of Anglo-Saxon craftsmanship found in Britain. This single discovery changed not only Britain’s perception of their past, but the very definition of “the dark ages.

The provision of health information to stroke patients within an acute hospital setting: What actually happens and how do patients feel about it?

This preliminary study describes how health information is provided to stroke patients in an acute hospital and describes their perceptions of health information provision. A further aim was to determine if patients with aphasia were disadvantaged in their receipt of information. Seven stroke patients were observed in hospital for an average of 102 minutes each and then interviewed using a semi-structured interview. When communication occurred, only 17.5% of communication time was spent providing information. Patients with aphasia received information for less time and on fewer topics. Implications regarding approaches to information provision for patients with and without aphasia are discussed

Environmental mastery and depression in older adults in residential care

This study investigated the association between environmental mastery and depression in a sample of 96 older adults (aged 64&ndash;98 years) in residential care. The participants completed a scale that assessed depression along with measures for risk factors for depression such as functional capacity, self-evaluated physical health, bereavement experiences and environmental mastery. The results showed that 49 per cent of the variance in participants&rsquo; scores in depression could be attributed to their self-reported level of environmental mastery. Given the complexity of depression and the likelihood of reduced environmental mastery among older adults in residential care, the construct was further assessed as a mediating variable between the risk factors and depression. With environmental mastery taken as such, the explained variance in depression increased to 56 per cent. It was concluded that environmental mastery may be one of the more important factors affecting the mental health of older adults living in residential care and that strategies for increasing the residents&rsquo; environmental mastery are important to their psychological wellbeing. The discussion notes that among the questions needing further investigation are whether older adults who experience high environmental mastery make the transition from community living to residential nursing home care more successfully than others, and whether perceived mastery diminishes over time or occurs at the point of transition from community independent living to dependent supported living.<br /

Proteasome-based selection systems for generation of recombinant CHOK1SV GS- KO™ cell lines with enhanced productivity

Chinese hamster ovary (CHO) cells are widely used industrially for the production of biotherapeutic proteins. In order to generate recombinant CHO cell lines expressing the target biotherapeutic gene(s) of interest, metabolic markers are used to select for those cells that have stably incorporated the gene(s) of interest. Such selection systems work very efficiently but do not directly select cells based upon secreted biotherapeutic recombinant protein productivity characteristics. When such biotherapeutic proteins are synthesised in eukaryotic cells, typically the polypeptide is co-translationally fed into the endoplasmic reticulum where it is folded, and if required, assembled with other polypeptides/domains, as in the case of antibodies. An overload of the capacity of the ER to fold and assemble recombinant proteins can result in upregulation of ER-associated degradation (ERAD) where unfolded or incorrectly folded or assembled material is retro-translocated out of the ER to the proteasome for degradation and recycling of amino acids. We have therefore investigated whether the susceptibility of cells to proteasome inhibitors during cell line construction when a recombinant load is placed upon the cell can be used to select for cells with a greater capacity for producing recombinant biotherapeutic proteins whilst maintaining or enhancing the quality of the material secreted. A number of proteasome inhibitors were therefore investigated, epoxomicin, MG-132 and bortezomib, at different concentrations to identify concentrations that would provide selection but not result in complete cell death. A range of concentrations was then added to CHO cells, along with MSX, during cell pool construction using Lonza’s CHOK1SV GS-KO™ proprietary host cell line to investigate whether this resulted in the generation of cell pools with enhanced productivity characteristics as compared to selection using MSX alone. Using this approach, and a number of different recombinant biotherapeutic molecules, we have found that CHO pools giving enhanced product concentrations can be generated (see Figure 1 for example) and validated this in ambr15 miniature bioreactor experiments. We have thus shown that stable transfectants derived from pools that had been cultured with proteasome inhibitors were more productive than pools generated without proteasome inhibitors. Further, stable transfectants generated using proteasome inhibitors retained their higher productivity characteristics even when the proteasome inhibitors were no longer added at subculture, meaning that proteasome inhibitors are only required in the initial stages of cell line construction. Please click Additional Files below to see the full abstract

Black Perspectives on Creativity, Trustworthiness, Welcome and Well-Being--Findings From a Qualitative Study

Culture + Community in a Time of Transformation: A Special Edition of Culture Track is a collaborative effort to keep the cultural sector in dialogue with its communities and participants during the pandemic and inform deeper equity and justice in the years to come. The project pivoted from examining public attitudes and behaviors in a "time of crisis" in 2020 to doing so in a "time of transformation" in 2021, with a crucial focus around racialized experiences in connection with cultural participation and cultural organizations.The first phase of the research, conducted in Spring 2020, was a large-scale survey intended to inform not just resilience but also innovation and progress toward equity in the cultural sector, and to give the U.S. public a voice in the future of cultural engagement. But that first phase was designed and conducted before the murder of George Floyd ignited a national upswell of anger, sadness, and activism and the Movement for Black Lives began to reshape the discourse around racism in every aspect of American life. In a follow-up statistical analysis of the same (early 2020) data published in December as "Centering the Picture," we and our colleagues explored respondents' experiences in relation to their racial and ethnic identities to highlight and amplify what people of color have been going through and what they would like to see changed in the future. The report revealed some unique experiences and perspectives that Black and African American adults in the U.S. have in relation to cultural engagement, digital connection with arts and culture, and social change. The Slover Linett team, knowing that qualitative methods would be necessary to understand those perspectives in a more nuanced and holistic way, advocated for an additional phase of research in 2021 that would offer a triangulation with — as well as departure point from — the twowave quantitative survey.To that end, and in order to authentically amplify Black voices and stories, we dedicated this qualitative phase of the research solely to Black and African American participants' perspectives, since those viewpoints have historically been excluded or sidelined in most research studies and planning efforts in the cultural field. We intentionally took a broad approach to this inquiry, exploring general dynamics of creativity, trustworthiness, welcome, and community support rather than focusing narrowly on arts and culture organizations and attendance. This allowed us to hear and explore how culture and community experiences and organizations naturally fit into peoples' lives, and it led to rich insights that can inform practice, funding, and policy

Gut microbiome in serious mental illnesses: A systematic review and critical evaluation.

Schizophrenia and bipolar disorder (BD) are associated with debilitating psychiatric and cognitive dysfunction, worse health outcomes, and shorter life expectancies. The pathophysiological understanding of and therapeutic resources for these neuropsychiatric disorders are still limited. Humans harbor over 1000 unique bacterial species in our gut, which have been linked to both physical and mental/cognitive health. The gut microbiome is a novel and promising avenue to understand the attributes of psychiatric diseases and, potentially, to modify them. Building upon our previous work, this systematic review evaluates the most recent evidence of the gut microbiome in clinical populations with serious mental illness (SMI). Sixteen articles that met our selection criteria were reviewed, including cross-sectional cohort studies and longitudinal treatment trials. All studies reported alterations in the gut microbiome of patients with SMI compared to non-psychiatric comparison subjects (NCs), and beta-diversity was consistently reported to be different between schizophrenia and NCs. Ruminococcaceae and Faecalibacterium were relatively decreased in BD, and abundance of Ruminococcaceae was reported across several investigations of SMI to be associated with better clinical characteristics. Lactic acid bacteria were relatively more abundant in SMI and associated with worse clinical outcomes. There was very limited evidence for the efficacy of probiotic or prebiotic interventions in SMI. As microbiome research in psychiatry is still nascent, the extant literature has several limitations. We critically evaluate the current data, including experimental approaches. There is a need for more unified methodological standards in order to arrive at robust biological understanding of microbial contributions to SMI

Regulatory interactions of αβ and γλ T cells in glomerulonephritis

Regulatory interactions of αβ and γλ T cells in glomerulonephritis.BackgroundSeveral lines of evidence suggest that cellular immune mechanisms contribute to glomerulonephritis.MethodsThe roles of αβ and γλ T cells in the pathogenesis of glomerulonephritis were investigated in a model of nephrotoxic nephritis in mice deficient in either T-cell population [T-cell receptor (TCR)β and TCRλ knockout mice]. The model, induced by the injection of rabbit anti-mouse glomerular basement membrane antibody, is characterized by the development of proteinuria and glomerular damage over a 21-day observation period in wild-type mice.ResultsMice deficient in either αβ or γλ T cells developed minimal proteinuria and glomerular lesions and had a significant reduction in macrophage accumulation compared with wild-type mice. In γλ T-cell–deficient mice, circulating levels and glomerular deposition of autologous IgG were comparable to wild-type levels, while αβ T-cell–deficient mice had no autologous IgG production. Autologous antibody production was not required for the development of glomerulonephritis since mice that lack IgG and B cells (μ-chain-/-) developed similar proteinuria to that observed in wild-type mice.ConclusionsThese studies suggest a proinflammatory role for both αβ and γλ T cells in glomerular injury, independent of the humoral response. This is the first demonstration, to our knowledge, that both T-cell subsets contribute to the progression of a disease, and it suggests that complex regulatory interactions between αβ and γλ T cells play a role in glomerular injury

Associations between women’s economic and social empowerment and intimate partner violence: findings from a microfinance plus program in rural North West Province, South Africa

Given the mixed evidence on whether women's economic and social empowerment is beneficial or not for reducing intimate partner violence (IPV), we explored the relationship between women?s empowerment and IPV risk. We analyzed data from baseline interviews with married women (n = 415) from the Intervention with Microfinance and Gender Equity (IMAGE) longitudinal study in rural South Africa. IMAGE combines a poverty-focused microfinance program with a gender-training curriculum. We fitted logistic regression models to explore associations between women?s economic situation/empowerment and IPV. For the multivariable logistic regression, we fitted three models that progressively included variables to explore these associations further. Women who reported ?few to many times? for not earning enough to cover their business costs faced higher odds of past year physical and/or sexual violence (adjusted odds ratio [aOR] = 6.1, 1.7-22.3, p = .01). Those who received a new loan experienced higher levels of past year emotional (aOR = 2.8, 1.1-7.4, p = .03) and economic abuse (aOR = 6.3, 2.2-18.5, p = .001). Women who reported that partners perceived their household contribution as not important faced higher odds of past year economic abuse (aOR = 2.8, 1.0-7.8, p = .05). Women who reported joint decision-making or partner making sole reproductive decisions reported higher levels of past year physical and/or sexual violence (aOR = 5.7, 0.9-39.4, p = .07) and emotional abuse (aOR = 3.0, 0.9-10.2, p = .08). Economic stress and aspects of women?s empowerment, alongside established gender roles within marital relationships is associated with IPV risk in rural South Africa. Although improved economic conditions for women appears to be protective against physical and sexual IPV, associations between certain indicators of women's economic situation, empowerment, and IPV are inconsistent. We need to consider complementary programming and all types of IPV in research, intervention, and policy, as different aspects of empowerment have varying associations with different types of IPV (physical, sexual, emotional, and economic abuse)

Absence of detectable monkeypox virus DNA in 11,000 English blood donations during the 2022 outbreak

BACKGROUND: A large, worldwide outbreak of mpox (formerly referred to as monkeypox) involving mainly men who have sex with men commenced in May 2022. We evaluated the frequency of positivity for the causative agent, monkeypox virus (MPXV), in blood donations collected in August 2022, during the outbreak period in Southern England. METHODS/MATERIALS: The sensitivity and specificity of an MPXV-specific PCR and a generic non-variola orthopoxvirus (NVO) PCR were evaluated using samples from mpox cases and synthetic DNA standards. Residual minipools from nucleic acid testing were obtained from 10,896 blood donors in Southern England, with 21% from London. RESULTS: MPXV and NVO PCRs were both capable of detection of single copies of target sequence with calculated limits of detection (LOD)90  s of 2.3 and 2.1 DNA copies and analytical sample sensitivities of 46 and 42 MPXV DNA copies/ml, respectively. 454 minipools produced from 10,896 unique donors were assayed for MPXV DNA by both methods. No positive minipools were detected by either PCR. CONCLUSIONS: Although blood donors are unrepresentative of the UK population in terms of MPXV infection risk, the uniformly negative MPXV DNA testing results provide reassurance that MPXV viraemia and potential transmission risk were rare or absent in donors during the outbreak period. Minipools from blood donors allow rapid implementation of large-scale population-based screening for emerging pathogens and represent an important resource for pandemic preparedness