Identification of a methylase required for 2-methylhopanoid production and implications for the interpretation of sedimentary hopanes

The rise of atmospheric oxygen has driven environmental change and biological evolution throughout much of Earth’s history and was enabled by the evolution of oxygenic photosynthesis in the cyanobacteria. Dating this metabolic innovation using inorganic proxies from sedimentary rocks has been difficult and one important approach has been to study the distributions of fossil lipids, such as steranes and 2-methylhopanes, as biomarkers for this process. 2-methylhopanes arise from degradation of 2-methylbacteriohopanepolyols (2-MeBHPs), lipids thought to be synthesized primarily by cyanobacteria. The discovery that 2-MeBHPs are produced by an anoxygenic phototroph, however, challenged both their taxonomic link with cyanobacteria and their functional link with oxygenic photosynthesis. Here, we identify a radical SAM methylase encoded by the hpnP gene that is required for methylation at the C-2 position in hopanoids. This gene is found in several, but not all, cyanobacteria and also in α -proteobacteria and acidobacteria. Thus, one cannot extrapolate from the presence of 2-methylhopanes alone, in modern environments or ancient sedimentary rocks, to a particular taxonomic group or metabolism. To understand the origin of this gene, we reconstructed the evolutionary history of HpnP. HpnP proteins from cyanobacteria, Methylobacterium species, and other α-proteobacteria form distinct phylogenetic clusters, but the branching order of these clades could not be confidently resolved. Hence,it is unclear whether HpnP, and 2-methylhopanoids, originated first in the cyanobacteria. In summary, existing evidence does not support the use of 2-methylhopanes as biomarkers for oxygenic photosynthesis

Fatigue damage assessment of a car body-in-white using a frequency-domain approach

This work presents an application of a frequency-domain methodology developed for the fatigue damage and service life assessment of mechanical components under multiaxial random loadings. The road-induced random loadings in a virtual laboratory bench test (four post test rig) are determined using an integrated MB/FE (Multi-Body/Finite Element) analysis. A method (i.e. the variance method) based on the statistics of the observed multiaxial loadings is used to determine the critical direction. The shear stress resolved on the critical direction is then assumed as the reference loading for the subsequent fatigue analysis. A frequency-domain approach recently proposed in the literature (i.e. the non-Gaussian TB method), capable to include the load non-normality into the fatigue assessment procedure, is used to estimate the loading spectrum. A comparison between the observed and the estimated loading spectrum, extrapolated from short to longer time (e.g. the entire vehicle service life), is shown. The presented results show how the proposed methodology could be a very useful tool for the reliable and quick analysis of components under multiaxial random loadings

Fatigue damage assessment of a car body-in-white using a frequency-domain approach

This work presents an application of a frequency-domain methodology developed for the fatigue damage and service life assessment of mechanical components under multiaxial random loadings. The road-induced random loadings in a virtual laboratory bench test (four post test rig) are determined using an integrated Multi-Body/Finite Element (MB/FE) analysis. A method (i.e. the variance method) based on the statistics of the observed multiaxial loadings is used to determine the critical direction. The shear stress resolved in the critical direction is then assumed as the reference loading for the subsequent fatigue analysis. A frequency-domain approach recently proposed in the literature (i.e. the non-Gaussian TB method), capable to include the load non-normality into the fatigue assessment procedure, is used to estimate the loading spectrum. A comparison between the observed and the estimated loading spectrum, extrapolated from shorter to longer time is shown. The presented results show how the proposed methodology could be a very useful tool for the reliable and quick analysis of components under multiaxial random loading

Engineering NixCoyP electrocalaysts for HER and OER in alkaline media

International audienc

How to build an information system application to the health domain.

Computer based information systems aim at improving knowledge, evaluation and management processes. Such tools are still developing in the non-profit sector. This paper aims at: a) reminding how to evolve from data to management information systems b) describing how to build a population based health information system c) describing the concepts required for conceiving a retrieval system and a system for inserting data d) documenting the importance of conceiving the system not only from a user driven perspective, but also taking into account the requirements of an information management system