Comparación de dos estilos de la enseñanza del Español como una lengua extranjera

Indiana University-Purdue University Indianapolis (IUPUI)El método de enseñanza de uso común en el aula de hoy, el comunicativo, se ve frecuentemente con algunas ciertas incongruencias. Esto a veces llega a ser una fuente de frustración para el/la profesor/a que quiere transmitir el lenguaje de una manera efectiva, pero que lo ve difícil a causa de lo que parece, según muchos, una falta de información muy clara acerca de cómo y cuándo enseñar las formas gramaticales. Las investigaciones ofrecen puntos de vista muy opuestos: hay mucho debate acerca de si se debe enseñar la gramática en primer lugar; y si se enseña, ¿cómo se enseña para alcanzar el producto final de un grupo de estudiantes que usa la lengua con fluidez? ¿Existe alguna fórmula mágica, algún método eficaz para contestar estas preguntas a la satisfacción de los que requieren evaluar algo tan difícil de medir? En búsqueda de alguna respuesta concreta, aquí se va a investigar dos modelos comunicativos de la enseñanza de la gramática: uno que aparece frecuentemente en los libros de textos comunicativos, y uno de llegada más recién- el de la Instrucción Basada en el Procesamiento del Input (IBPI) de Bill VanPatten (1996, 2004a, 2004b, 2005). Las metas de esta investigación son dos. Primero, se va a demostrar las diferencias metodológicas entre la IBPI y la instrucción comunicativa estándar con lecciones explícitas. Además, se va a explorar la eficacia de estos dos métodos pedagógicos en la enseñanza de un punto gramatical con un estudio piloto de pequeña escala

West Nile Virus Detection in Urine

We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase–polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have ≥99% similarity to the WNV strain NY 2000-crow3356

Transcriptomic analysis of the late stages of grapevine (Vitis vinifera cv. Cabernet Sauvignon) berry ripening reveals significant induction of ethylene signaling and flavor pathways in the skin

Background: Grapevine berry, a nonclimacteric fruit, has three developmental stages; the last one is when berrycolor and sugar increase. Flavors derived from terpenoid and fatty acid metabolism develop at the very end of thisripening stage. The transcriptomic response of pulp and skin of Cabernet Sauvignon berries in the late stages ofripening between 22 and 37 \ub0Brix was assessed using whole-genome micorarrays.Results: The transcript abundance of approximately 18,000 genes changed with \ub0Brix and tissue type. There were alarge number of changes in many gene ontology (GO) categories involving metabolism, signaling and abioticstress. GO categories reflecting tissue differences were overrepresented in photosynthesis, isoprenoid metabolismand pigment biosynthesis. Detailed analysis of the interaction of the skin and pulp with \ub0Brix revealed that therewere statistically significantly higher abundances of transcripts changing with \ub0Brix in the skin that were involved inethylene signaling, isoprenoid and fatty acid metabolism. Many transcripts were peaking around known optimalfruit stages for flavor production. The transcript abundance of approximately two-thirds of the AP2/ERF superfamilyof transcription factors changed during these developmental stages. The transcript abundance of a unique clade ofERF6-type transcription factors had the largest changes in the skin and clustered with genes involved in ethylene,senescence, and fruit flavor production including ACC oxidase, terpene synthases, and lipoxygenases. The transcriptabundance of important transcription factors involved in fruit ripening was also higher in the skin.Conclusions: A detailed analysis of the transcriptome dynamics during late stages of ripening of grapevine berriesrevealed that these berries went through massive transcriptional changes in gene ontology categories involvingchemical signaling and metabolism in both the pulp and skin, particularly in the skin. Changes in the transcriptabundance of genes involved in the ethylene signaling pathway of this nonclimacteric fruit were statisticallysignificant in the late stages of ripening when the production of transcripts for important flavor and aroma compoundswere at their highest. Ethylene transcription factors known to play a role in leaf senescence also appear to play a role infruit senescence. Ethylene may play a bigger role than previously thought in this non-climacteric fruit