完全長転写産物の網羅的解析による肝細胞癌における新規スプラシング異常と発がん性転写産物の解明

京都大学新制・課程博士博士(医学)甲第24783号医博第4975号新制||医||1066(附属図書館)京都大学大学院医学研究科医学専攻(主査)教授 村川 泰裕, 教授 波多野 悦朗, 教授 小川 誠司学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

On the Mourre estimates for Floquet Hamiltonians

In the spectral and scattering theory for a Schrödinger operator with a time-periodic potential H(t)=p²/2+V(t, x) , the Floquet Hamiltonian K=−i∂t+H(t) associated with H(t) plays an important role frequently, by virtue of the Howland–Yajima method. In this paper, we introduce a new conjugate operator for K in the standard Mourre theory, that is different from the one due to Yokoyama, in order to relax a certain smoothness condition on V

Protective Effects of Vitamin E Analogs against Carbon Tetrachloride-Induced Fatty Liver in Rats

Recently, it has been reported that α-tocopherol (α-Toc) is effective for amelioration of liver damage. However, it is unknown whether other vitamin E analogs are effective. In this study, we investigated the effects of γ-tocopherol (γ-Toc) and tocotrienols (T3) in rats with fatty liver. Rats fed a vitamin E-deficient diet for four weeks were divided into eight groups: Control, carbon tetrachloride (CCl4), α-Toc, α-Toc + CCl4, γ-Toc, γ-Toc + CCl4, T3 mix, T3 mix + CCl4. After a 24 h fast, the rats were administered 20 mg of each of the vitamin E analogs, respectively. Moreover, the CCl4 group were given 0.5 ml/kg body weight corn oil preparation containing CCl4 6 h after vitamin E administration. We measured the activities of aspartate aminotransferase and alanine aminotransferase (ALT) in plasma, and the contents of triglyceride (TG), total cholesterol (T-Chol) and vitamin E analogs in the liver. Also, we determined the hepatic expression of mRNA for inflammatory cytokines. The liver TG content in the γ-Toc + CCl4 and T3 mix + CCl4 groups was decreased in comparison with the CCl4 group. Moreover, ALT activity in the T3 mix + CCl4 group was significantly lower than CCl4 group. These findings suggest that γ-Toc and T3 are effective for amelioration of fatty liver

Apocope of Late Old Chinese short *ă: Early Central Asian loanword and Old Japanese evidence for Old Chinese disyllabic morphemes*

The morphophonology of Old Chinese has usually been reconstructed as an earlier version of the traditional reconstruction of Middle Chinese, with exclusively monosyllabic morphemes. For Old Chinese some scholars have posited syllabic morphemes with long or short vowels, or even polysyllabic morphemes, and other theories have been proposed, but it is still assumed that by Late Old Chinese any longer morphemes that once existed were already monosyllabic. However, Central Asian loans in Chinese suggest some disyllabic morphemes still existed in Late Old Chinese. They seem to be confirmed by a new study of little-noted Old Japanese transcriptions known as nigōgana. Thus the hitherto problematic Old Chinese and Old Japanese transcriptions of foreign words such as Saka and Buddha, and the monosyllabic theory of Old Chinese morphology, must be reconsidered. This paper’s new reconstructions conform to the data and have great implications for the reconstruction of Old Chinese as well as for the reconstruction of the early Chinese loans into Japanese

Affinity for α-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs

Abstractα-Tocopherol transfer protein (αTTP), a product of the gene which causes familial isolated vitamin E deficiency, plays an important role in determining the plasma vitamin E level. We examined the structural characteristics of vitamin E analogs required for recognition by αTTP. Ligand specificity was assessed by evaluating the competition of non-labeled vitamin E analogs and α-[3H]tocopherol for transfer between membranes in vitro. Relative affinities (RRR-α-tocopherol=100%) calculated from the degree of competition were as follows: β-tocopherol, 38%; γ-tocopherol, 9%; δ-tocopherol, 2%; α-tocopherol acetate, 2%; α-tocopherol quinone, 2%; SRR-α-tocopherol, 11%; α-tocotrienol, 12%; trolox, 9%. Interestingly, there was a linear relationship between the relative affinity and the known biological activity obtained from the rat resorption-gestation assay. From these observations, we conclude that the affinity of vitamin E analogs for αTTP is one of the critical determinants of their biological activity

γ-Tocopherol Accelerated Sodium Excretion in a Dose-Dependent Manner in Rats with a High Sodium Intake

We have previously reported that γ-tocopherol (γ-Toc) displays a natriuretic potency in rats fed a NaCl diet and administered 20 mg γ-Toc. In this study, we investigated whether γ-Toc has natriuretic potency at a dose lower or higher than 20 mg in rats given a NaCl diet. Male rats were fed a control diet or a NaCl diet and administered either placebo or 10, 20 or 40 mg of γ-Toc. The rat urine was collected for 24 hours (divided into 6 hour periods) and the 2,7,8-trimethyl-2-(2'-carboxyethyl)-6-hydroxychroman (γ-CEHC) level, the sodium excretion content, and the urine volume were determined. The 24-hour γ-CEHC and sodium levels in the urine of the NaCl groups given 20 mg or 40 mg γ-Toc were significantly higher than those in the placebo group. The peak levels of urine sodium and γ-CEHC in the NaCl group given 40 mg γ-Toc appeared at 0–6 h, which was a more rapid increase than that seen in the group given 20 mg γ-Toc. The 24-hour urine volumes of the NaCl groups given 10 and 20 mg γ-Toc were significantly higher than the urine volume of the placebo group. Our findings suggested that γ-Toc increased sodium excretion in a dose-dependent manner in rats fed a NaCl diet. Moreover, a high dose of γ-Toc may accelerate its metabolism and cause an increase in the rate of sodium excretion