Analysis for Molecular Distinction in the Chloroplast DNA Sequences of Gymnospora montana (Celastraceae) and Belanites aegyptiaca (Balanitaceae) from Semi-arid Area

Gymnospora montana (Celastraceae) and Belanites aegyptiaca  (Balanitaceae) showed marked similarity in  their cpDNA sequences. Therefore, its detail analysis of cpDNA sequences is performed for codon use bias and its index, relative synonymous codon use value (RSCU), effective number of codons (ENC), GC content of the gene and frequencies of the nucleotides G+C at various positions in synonymous codon were calculated and compared it with Tribulus terresties. Length of the gene and ENC showed close relationship which suggest that longer genes has less codon bias. The codons for leucine, isoleucine and serine were most abundant in the studied plant species. The correlation analysis suggested that codon usage patterns in both cp genomes appear due to the different forces; natural selection, mutation pressure, GC content of gene and protein length.   Their role in gene evolution process is discussed

Standardization of leaf age for radical scavenging activity in Terminalia arjuna

Terminalia arjuna is one of the many such plant that used in indigenous system of medicine for curing different diseases. For effective formulation of herbal drug selection of proper age of the plant is imperative. In the present study, the T. arjuna plant (leaves) was collected from the Botanical garden of Saurashtra University. The leaves were grouped in to various size and growth analysis was performed. Each extract homogenized in methanol and was used for chlorophyll estimation and antioxidant activities. Radical scavenging activity of the methanol extract of different stages leaves were determined using a stable free radical 2, 2-diphenyl-1-picryl hydrazyl (DPPH), using Micro plate assay. Further the phenolic compounds were separated by Reverse phase high pressure liquid chromatography analysis (RP-HPLC). At the leaf stage when maximum antioxidant activity was observed, phenol like Gallic acid showed distinct peak. The role of phenolic compounds in antioxidant activities is discussed

Molecular and Computational Analysis of Chlorophyll Pigment-binding Protein cp47 from Selected Species of Semi Arid Region of Western India

Photosynthesis means “synthesis with the help of light”, involves the composite functioning of various protein complexes. CP47 is a pigment-binding protein of PSII of a molecular mass of about 56 kDa. CP47, encoded by the chloroplastic psbB gene, is an integral part of the oxygen-evolving complex of PS -II centres. In the present study, analysis of a psbB gene was performed from various tree, shrub, vine and herb species of Saurashtra region. The genomic DNA was isolated from the 46 samples and psbB gene was amplified using specific primers (60R-61F) in PCR. The amplified gene was sequenced from all plant samples and submitted to NCBI database. The length of the amplified sequence was ~300 bp, was translated to the protein sequence. The obtained sequences were analyzed with the help of CPH and Pyre2 tools. The Pyre2 tools showed 40 reliable structure prediction out of 46. ProtParam was used for carrying out the protein physico-chemical analysis of all the proteins showing variations in the protein properties. The number of residues in favored region, as observed in the Ramachandran plot analysis, indicates reliability of the protein structure prediction. The obtained results for the sequence and structure analyses may help to understand the functional application of these proteins

Biochemical and Immunological Studies on Plant pathogenic organisms

Not availabl

Genome sequence of Ochrobactrum anthropi strain SUBG007, a plant pathogen and potential xenobiotic compounds degradation bacterium

Ochrobactrum anthropi SUBG007 was isolated from the fruit of Prunus dulcis in Rajkot (22.30°N, 70.78°E), Gujarat, India. Here we present the 4.37 Mb genome sequence strain SUBG007, which may provide the genetic information for the application in environment pollution degradation and agriculture field. The strain also posses many genes cluster which involved in production of important secondary metabolites. The nucleotide sequence of this genome was deposited into NCBI GenBank under the accession LUAY00000000