Three new p-fluorophenylalanine resistant (fpr) mutants

Three new fpr mutant

Law Enforcement and Executive Order: Duplication in Missouri’s Prescription Drug Monitoring Program

Missouri had long been scrutinized as the only state operating without a prescription drug monitoring program. These programs are seen as an effective way to monitor prescription opioids as opioid-related deaths have risen in the past decade. The opioid crisis has gained significant media attention and cast scrutiny on pharmaceutical companies, physicians, and state and federal governments. This comment explores the history of the opioid crisis and details Missouri’s struggle to implement a prescription drug monitoring program legislatively. In 2017, former Governor Eric Greitens signed an Executive Order directing the Missouri Department of Health and Senior Services to implement one of these programs. This program is centered on law enforcement, and as written, aims to monitor and collect data that is already available via alternative means. For Missouri to successfully combat the opioid crisis, the state needs to utilize resources already at its disposal to create an effective means of monitoring prescription opioids. At the time of publication, the program had been implemented; however, there is no available data currently available to determine the effectiveness of the program

Measurements of the spatial structure and directivity of 100 KeV photon sources in solar flares using PVO and ISEE-3 spacecraft

The objective of this grant was to measure the spatial structure and directivity of the hard X-ray and low energy gamma-ray (100 keV-2 MeV) continuum sources in solar flares using stereoscopic observations made with spectrometers aboard the Pioneer Venus Orbiter (PVO) and Third International Sun Earth Explorer (ISEE-3) spacecraft. Since the hard X-ray emission is produced by energetic electrons through the bremsstrahlung process, the observed directivity can be directly related to the 'beaming' of electrons accelerated during the flare as they propagate from the acceleration region in the corona to the chromosphere/transition region. Some models (e.g., the thick-target model) predict that most of the impulsive hard X-ray/low energy gamma-ray source is located in the chromosphere, the effective height of the X-ray source above the photosphere increasing with the decrease in the photon energy. This can be verified by determining the height-dependence of the photon source through stereoscopic observations of those flares which are partially occulted from the view of one of the two spacecraft. Thus predictions about beaming of electrons as well as their spatial distributions could be tested through the analysis proposed under this grant

Epsilon-Near-Zero Al-Doped ZnO for Ultrafast Switching at Telecom Wavelengths: Outpacing the Traditional Amplitude-Bandwidth Trade-Off

Transparent conducting oxides have recently gained great attention as CMOS-compatible materials for applications in nanophotonics due to their low optical loss, metal-like behavior, versatile/tailorable optical properties, and established fabrication procedures. In particular, aluminum doped zinc oxide (AZO) is very attractive because its dielectric permittivity can be engineered over a broad range in the near infrared and infrared. However, despite all these beneficial features, the slow (> 100 ps) electron-hole recombination time typical of these compounds still represents a fundamental limitation impeding ultrafast optical modulation. Here we report the first epsilon-near-zero AZO thin films which simultaneously exhibit ultra-fast carrier dynamics (excitation and recombination time below 1 ps) and an outstanding reflectance modulation up to 40% for very low pump fluence levels (< 4 mJ/cm2) at the telecom wavelength of 1.3 {\mu}m. The unique properties of the demonstrated AZO thin films are the result of a low temperature fabrication procedure promoting oxygen vacancies and an ultra-high carrier concentration. As a proof-of-concept, an all-optical AZO-based plasmonic modulator achieving 3 dB modulation in 7.5 {\mu}m and operating at THz frequencies is numerically demonstrated. Our results overcome the traditional "modulation depth vs. speed" trade-off by at least an order of magnitude, placing AZO among the most promising compounds for tunable/switchable nanophotonics.Comment: 14 pages, 9 figures, 1 tabl

2007 Supermarket Panel Report

Replaced with revised version of paper 12/16/10.Agribusiness, Industrial Organization,

Identification and cloning of the Neurospora crassa glyceraldehyde-3-phosphate dehydrogenase gene, gpd-1

In work initially intended to use the am gene coding sequences as a reporter gene, 5’ RACE PCR (Frohman et al., 1988 Proc. Natl. Acad. Sci. USA. 85:8998-9002) with three gene specific nested primers was performed. The product was cloned and sequenced, but found not to represent the am gene. Comparison to sequences in Genbank revealed that the product could encode a product homologous to glyceraldehyde-3-phosphate dehydrogenase (GPD) from a variety of other organisms. Consequently the PCR product was used to screen a lambda gt-11 expression library (Sachs et al. 1986 J. Biol. Chem 261:869-873). The 1.3 kb insert from one cDNA clone was sequenced (Figure 1) and used to screen a Neurospora genomic library made in an EMBL-3 vector by E. Cambareri. All of the positive clones had a 7 kb BamHI fragment. Relevant portions of one of the genomic clones was sequenced (Figure 1) revealing two introns. Although the complete genomic clone was not sequenced, comparison of restriction fragments from the cDNA and genomic clones indicated that no other introns are present in the Neurospora gpd-1 gene

Fungal Genetics Stock Center Catalogue of Strains, 9th edition

Catalogue of Strains, 9th edition, 2002, supplement to Fungal Genetics Newsletter No. 49. This catalogue contains lists of materials held by the Fungal Genetics Stock Center

An ultra-fast method of DNA extraction from Neurospora

We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) while giving excellent yield and size of DNA, is somewhat cumbersome and also results in the occasional sample that proves to be uncuttable