The Grizzly, January 24, 1995

Ursinus College and the U.S. News and World Report Rating System • Ursinus Concludes The Next Step Campaign • 4,000 Casualties in Japanese Earthquake • Newt Confronts Ethics Issues • Local Bank Robber Brought Up on Federal Charges • Politics Department Update • Servant-Leadership Program Comes to Ursinus • St. Andrew\u27s Society of Philadelphia Scholarships • An Afternoon with Picasso and Moore • Thirdgill Kicks Off This Semester\u27s Comedy • Star Trek: Voyager Launches • Support Your Coffee House • Not-So-Super Super Bowl • Alumnus Expresses Concern Over Oversight • Men\u27s B-Ball Snaps Two-Game Skid • Bears Ready For Conference Showdown with Muhlenberg • Gymnasts Off to a Raging Start • Bears Win Triangular Meet • Aqua Bears Swept by Gettysburg • Lady Bears are Ranked For the First Timehttps://digitalcommons.ursinus.edu/grizzlynews/1350/thumbnail.jp

The Grizzly, September 13, 1994

Budget Concerns Discussed • The Search Continues • Convocation Opens Academic Year • Military Action Likely • U.S. Relations with Cuba • Sculptures, Sculptures Everywhere • All Lines Busy • Capital Improvements • Excellence Should not be Overlooked • Things That I Like and Don\u27t Like About the Morning • Summer Entertainment Review \u2794: Movies; Concerts • DeLuca Dazzles Audience • Moments in Time • Around the NFL • Bears Fall in Season Openerhttps://digitalcommons.ursinus.edu/grizzlynews/1339/thumbnail.jp

The Grizzly, September 20, 1994

Sweeping Changes for Pledging • Berry \u27Suspends\u27 Campaign • Selling of a Folk Tradition • Clinton Shows Decisiveness • Plane Crashes Into White House • Campus Organizations Unite • Sororities: Disturbing the Peace or Boosting Self-Esteem? • Attention Seniors! • Ursinus Represented at Miss America Pageant • Apathy • Wismer Observations • A Personal Look at Who\u27s Among Us • New Works at Berman • CAB Update • Jazz Group Bright Moments to Perform Saturday • Voice of Ursinus Reborn • Ellie Keeps Mail Room Running Smoothly • The Lights are on, but Nobody\u27s Home • Program Offers Cultural Exchange • Martial Arts Club Available for Students • Taking Advantage of an Opportunity • UC Field Hockey Blanks Davis and Elkins • UC Football Impressive in Winhttps://digitalcommons.ursinus.edu/grizzlynews/1340/thumbnail.jp

The Grizzly, October 4, 1994

1994 Homecoming Court Spotlighted • Research Opportunities • Family Day \u2794 a Success • Arms Embargo Still Intact • Mining Industry Saved From Taxation • Homecoming Queen Nominees • New Lab Expands Horizons of Freshman Biologists • Recycling Lets Everyone Breathe a Little Easier • Kilmartin Lightens up Lower Lounge • Bright Moments and Sweet Sounds • Executing Justice with Pro-Theatre • Suggestions, Please! • Exploring Secrets & Truths Otherwise Unknown • Jam at the Trench • A Lesson in Life • The Real World: Collegeville • Prejudice Can Eclipse Unique Differences • Swarthmore Hands Bears Second Straight Defeat • UC Volleyball Loses Pair • UC Captain\u27s Council Set to Make Changeshttps://digitalcommons.ursinus.edu/grizzlynews/1342/thumbnail.jp

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Bilayer Edge and Curvature Effects on Partitioning of Lipids by Tail Length: Atomistic Simulations

The partitioning of lipids among different microenvironments in a bilayer is of considerable relevance to characterization of composition variations in biomembranes. Atomistic simulation has been ill-suited to model equilibrated lipid mixtures because the time required for diffusive exchange of lipids among microenvironments exceeds typical submicrosecond molecular dynamics trajectories. A method to facilitate local composition fluctuations, using Monte Carlo mutations to change lipid structures within the semigrand-canonical ensemble (at a fixed difference in component chemical potentials, Δμ), was recently implemented to address this challenge. This technique was applied here to mixtures of dimyristoylphosphatidylcholine and a shorter-tail lipid, either symmetric (didecanoylphosphatidylcholine (DDPC)) or asymmetric (hexanoyl-myristoylphosphatidylcholine), arranged in two types of structure: bilayer ribbons and buckled bilayers. In ribbons, the shorter-tail component showed a clear enrichment at the highly curved rim, more so for hexanoyl-myristoylphosphatidylcholine than for DDPC. Results on buckled bilayers were variable. Overall, the DDPC content of buckled bilayers tended to exceed by several percent the DDPC content of flat ones simulated at the same Δμ, but only for mixtures with low overall DDPC content. Within the buckled bilayer structure, no correlation could be resolved between the sign or magnitude of the local curvature of a leaflet and the mean local lipid composition. Results are discussed in terms of packing constraints, surface area/volume ratios, and curvature elasticity