Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Proceedings of the 13th annual conference of INEBRIA

CITATION: Watson, R., et al. 2016. Proceedings of the 13th annual conference of INEBRIA. Addiction Science & Clinical Practice, 11:13, doi:10.1186/s13722-016-0062-9.The original publication is available at https://ascpjournal.biomedcentral.comENGLISH SUMMARY : Meeting abstracts.https://ascpjournal.biomedcentral.com/articles/10.1186/s13722-016-0062-9Publisher's versio

ErbB2 initiated tumors with comparable latency in MMTV-<i>tva</i> and K6a-<i>tva</i> mice.

<p><b>(A)</b> Kaplan-Meier tumor-free survival curves of RCAS-ca<i>ErbB2</i>-infected mice of the indicated genotypes. <b>(B)</b> Immunofluorescent staining for HA-tagged ErbB2 in tumors from MMTV-<i>tva</i> and K6a-<i>tva</i> mice (scale bar: 50 um). Antibody specificity was determined by staining early lesions expressing HA-tagged ErbB2 (third panel, bottom left; positive control) and normal ducts (third panel, top right; negative control).</p