Phycocyanin as a proxy for algal blooms in surface waters: case study of Ukerewe Island, Tanzania

Knowledge of the parameters that contribute to water body eutrophication is essential for proper monitoring and management of water quality for human consumption. This study assessed water quality parameters in relation to phycocyanin (PC) as a proxy indicator for harmful algal blooms (HABs). Samples were collected from 23 water sources – lakes, wells, springs and boreholes – in selected villages, for six months. Parameters measured included temperature, pH, redox potential, dissolved oxygen, electrical conductivity, total dissolved solids, nitrate nitrogen, nitrite nitrogen, phosphorus, reactive phosphate and total chlorophyll, which were related to (PC) occurrence. The PC concentration detected in Lake Victoria ranged from 5 to 58.4 μg/l above the WHO alert level and exceeded that in other water sources by almost 30 μg/l (P < 0.001). Univariate relationship between water quality parameters and PC indicates association with temperature, redox potential, total chlorophyll, nitrate nitrogen, nitrite nitrogen, phosphate and reactive phosphorus (P < 0.001). The multivariate model indicates that redox potential, nitrate nitrogen and phosphorus are significant statistically (P < 0.05). A predictive model indicates that nitrate nitrogen and reactive phosphorus contribute significantly to PC occurrence whereby unit (1 mg/l) increases in these parameters increase PC by 9.55 and 4.38 μg/l (P < 0.05) respectively. This study demonstrates that water quality parameters can be used to predict increases in PC and hence as a proxy for HABs. It remains important to be able to classify algal blooms, to understand which species are present and their potential cyanotoxin production

Post-harvest interventions decrease aflatoxin and fumonisin contamination in maize and subsequent dietary exposure in Tanzanian infants : a cluster randomised-controlled trial

A cluster randomised controlled trial was performed in three agro-ecological zones of Tanzania to evaluate the effectiveness of locally available post-harvest mitigation strategies in preventing and reducing aflatoxin and fumonisin contamination in maize. A total of 300 children, each from one household, were randomly selected from 30 villages (intervention: n=15). The mitigation strategies focused on hand sorting (prior to storage and use), drying maize on mat/raised platforms, proper sun drying, application of storage insecticides and de-hulling before milling. Maize sample was collected from each household at harvest (baseline) and six months after harvest. Maize intake by each child, estimated using the 24 h dietary recall technique and its body weight measured using standard procedures were taken at six months after harvest. Aflatoxins and fumonisins in the maize samples were determined using HPLC. Follow-up (six month after harvest) data were available for 261 of the 300 households (intervention: n=136). Mean concentration of aflatoxins, or fumonisins was significantly (P<0.05) lower in the intervention than in the control group: intervention effects: mu g/kg (95% confidence interval (CI)) -4.9 (-7.3,-2.5), and -405, (-647,-162), respectively. The difference corresponds to 83 and 70% for aflatoxins, and fumonisins, respectively. At the end of the intervention, aflatoxin and fumonisin estimated mean intakes were lower in the intervention than in the control group by 78 and 65%, respectively. Six months after harvest, prevalence of underweight in the intervention group was 6.7% lower (P=0.014) than in the control group. Mean weight-for-age Z-score difference between the groups was 0.57 (95% CI; 0.16,-0.98; P=0.007). Post-harvest practices are effective in preventing and reducing aflatoxin and fumonisin contamination in maize and subsequent dietary exposure to infants. The interventions may be applied in these and other communities with similar environmental conditions or agricultural practices that favour production of aflatoxin and fumonisins in food crops

Harmful algal bloom and associated health risks among users of Lake Victoria freshwater: Ukerewe Island, Tanzania

There is a global concern regarding the occurrences of harmful algal blooms (HABs) and their effects on human health. Lake Victoria (LV) has been reported to face eutrophication challenges, resulting in an increase of bloom-forming cyanobacteria. This study is aimed at understanding the association of HABs and health risks at Ukerewe Island. A cross-sectional study conducted on 432 study subjects and water samples for cyanobacteria species identification were collected at LV shores. The results reveal that concentrations of cyanobacteria cells are beyond (WHO) acceptable limits; species of Microcystis aeruginosa range from 90,361.63 to 3,032.031.65 cells/mL and Anabaena spp. range from 13,310.00 to 4,814,702 cells/mL. Water usage indicates that 31% use lake water, 53% well water and 16% treated supplied pipe water. Vomiting and throat irritation was highly reported by lake water users as compared to wells and pipe water (P < 0.001). Gastrointestinal illness (GI) was significantly elevated among lake water users as compared to pipe and well water users (P < 0.001). Visible blooms in lake water were associated with GI, skin irritation and vomiting as compared to water without visible blooms (P < 0.001). The concentration of cyanobacteria blooms poses greater risks when water is used without treatment

Probabilistic dietary based estimation of the burden of aflatoxin-induced hepatocellular carcinoma among adult Malawians

This research article published by Wageningen Academic Publishers, 2019The risk of aflatoxin-induced hepatocellular carcinoma (HCC) among adults (average body weight of 60 kg) in Malawi was assessed based on aflatoxin B1 (AFB1) exposure through groundnut and maize consumption, by Monte Carlo simulation. The risk (cases per year per 100,000 people) of aflatoxin-induced HCC was estimated based on the AFB1 exposures estimated by this study and hepatitis B virus infection prevalence published for Malawi. AFB1 exposures were estimated by probabilistically combining data of AFB1 contamination in 338 groundnut and 604 maize samples with data of per capita groundnut and maize consumption in 274 households. Aflatoxins in the samples were analysed using validated LC-MS/MS, HPLC and VICAM based methods. The groundnut and maize consumption survey was based on household expenditure technique. The simulated mean AFB1 exposures through consumption of groundnuts, maize, and combination thereof were 28±65, 42±174, and 71±211 ng/kg. body weight (bw)/day, respectively. The estimated HCC risks were 1.26±2.72, 1.86±6.66 and 3.10±6.85 cases per 100,000 persons per year, respectively. Further, hypothetical eradication of hepatitis B virus (HBV) reduced the risk of HCC by 78%. This reaffirms the need for integrating HBV vaccination in the fight of aflatoxin induced HCC

Aflatoxin contamination in Tanzania: quantifying the problem in maize and groundnuts from rural households

Published online: 28 Apr 2021Aflatoxins are toxic and carcinogenic secondary metabolites, produced by Aspergillus flavus and Aspergillus parasiticus, which contaminate food and feed and threaten human and animal health. To assess the prevalence of aflatoxins in Tanzania, 180 groundnut and 200 maize samples were collected from 9 and 10 districts, respectively. Aflatoxin contamination was quantified using high performance liquid chromatography. Aflatoxins were detected in samples collected from all districts and prevalence ranged from 92 to 100% for groundnuts and 10 to 80% for maize. The mean aflatoxin level for groundnuts was 6.37 μg/kg and the highly contaminated sample had 40.31 μg/kg. For maize, the mean aflatoxin level was 12.47 μg/kg and the highly contaminated sample had 162.40 μg/kg. The estimated average probable daily intake (APDI) of aflatoxin B1 (AFB1) from groundnuts consumption was 1.88 ng/kg body weight/day, while for maize, it ranged between 151.98-272.89 ng/kg body weight/day. The APDI for both groundnut and maize exceeded the provisional maximum tolerable daily intake (PMTDI) of AFB1 for adults (1 ng/kg body weight/day), bringing about health concerns for populations in Tanzania. Another alarming finding was that 75% of the farmers who provided samples for analysis were not aware of aflatoxins or the negative health impacts from consuming contaminated products. Results reported in this paper show that aflatoxin contaminated staple crops are widely distributed in Tanzania and that the risk of human exposure is high due to diet preferences. Awareness campaigns are required to inform and protect farmers and consumers

Estimating the health burden of aflatoxin attributable stunting among children in low income countries of Africa

Numerous population-based studies have documented high prevalence of aflatoxin associated childhood stunting in low income countries. We provide an estimate of the disease burden of aflatoxin related stunting using data from the four African countries. For this empirical analysis, we obtained blood aflatoxin albumin adduct biomarker based exposure data as measured using ELISA technique and anthropometric measurement data from surveys done over a 12-year period from 2001 to 2012 in four low income countries in Africa. We used these data to calculate population attributable risk (PAR), life time disease burden for children under five by comparing two groups of stunted children using both prevalence and incidence-based approaches. We combined prevalence estimates with a disability weight, measuring childhood stunting and co-occurrence of stunting-underweight to produce years lived with disability. Using a previously reported mortality, years of life lost were estimated. We used probabilistic analysis to model these associations to estimate the disability-adjusted life-years (DALYs), and compared these with those given by the Institute for Health Metrics and Evaluation’s Global Burden of Disease (GBD) 2016 study. The PAR increased from 3 to 36% for aflatoxin-related stunting and 14–50% for co-occurrence of stunting and underweight. Using prevalence-based approach, children with aflatoxin related stunting resulted in 48,965.20 (95% uncertainty interval (UI): 45,868.75–52,207.53) DALYs per 100,000 individuals. Children with co-occurrence of stunting and underweight due to exposure to aflatoxin resulted in 40,703.41 (95% UI: 38,041.57–43,517.89) DALYs per 100,000 individuals. Uncertainty analysis revealed that reducing aflatoxin exposure in high exposure areas upto non-detectable levels could save the stunting DALYs up to 50%. The burden of childhood all causes stunting is greater in countries with higher aflatoxin exposure such as Benin. In high exposure areas, these results might help guide research protocols and prioritisation efforts and focus aflatoxin exposure reduction. HEFCE Global Challenge Research Fund Aflatoxin project

Heavy Metals Contamination in Agricultural Soil and Rice in Tanzania: A Review

Research Article published by Science Publishing GroupHeavy metals contamination in agricultural soil is a potential environmental threat to the safety of agricultural food crops such as rice which is consumed by majority of Tanzanians. The aim of this review is to put together available information on sources of heavy metals, their extent of contamination in agricultural soil and in rice, the risk of exposure through rice consumption as well as the relationship between heavy metals contamination in agricultural soils and in rice in Tanzania. There are several methods of determining the concentrations of heavy metals in soils and in rice. These include Inductively Coupled Plasma Mass Spectrometer (ICP – MS) and Energy Dispersive X – ray Fluorescence spectrometer (EDXRF). It has been mostly reported that the extent of heavy metals contamination in agricultural soils is influenced by their closeness to mining or industrial areas. The use of river waters in mining areas or wastewater from industries for irrigation has been associated to increasing levels of heavy metals in agricultural soils. The elevated level of heavy metals in agricultural soils leads to their accumulation in crops especially rice which upon consumption poses health effects to human and the ecosystem at large. This review suggests the need for determining the extent of heavy metals contamination in agricultural soils around potential areas such as mining and to link this with exposure assessment on heavy metals through rice consumption in Tanzania. This information is necessary to establish the extent at which rice consumers in Tanzania are at risk of heavy metals exposure

Pesticide Residues in Vegetables: Practical Interventions to Minimize the Risk of Human Exposure in Tanzania

Research Article published by Current Journal of Applied Science and TechnologyMalpractices in the use of pesticides in vegetable production have been reported in the horticultural sector in developing countries. This can result in excessive use of pesticides and, subsequently, in unacceptable levels of pesticide residues in foods of horticultural origin. Consumption of vegetables containing unacceptable levels of pesticide residues is of public concern due to its potentially harmful effects on human health. In this work, we reviewed the current status of pesticide application, the occurrence and exposure of pesticide residues in vegetables as well as factors influencing the problem of pesticide exposure in Tanzania. The review has realized that pesticides are rarely applied to vegetables following good agricultural practices. Further, pesticide residues in vegetables are not monitored and exposure studies are limited. Studies on the influence of vegetable processing on pesticide residues at household level have been done at laboratory scale. However, the influence of these processes to the residues at the community level is unknown. The review suggests the need for broader research on the pesticide application practices to establish the important practices that have significant association with the occurrence of pesticide residues in vegetables. Results from this research will allow for the allocation of resources for improvement, monitoring and control of these practices to minimize the risk of unwanted pesticide residues in vegetables. Continuous monitoring of pesticide residues in food, as well as the correlated human dietary exposure, is highly recommended in order to inform policymakers and risk managers of the status of the risk of exposure to pesticide residues

Human exposure to fumonisins from home grown maize in Tanzania

Fumonisins contaminate maize worldwide resulting in unacceptable fumonisin exposures in people relying on maize as staple food. This study determined fumonisins B1 (FB1) and B2 (FB2) in maize from 120 rural households: 30 from each of four main maize producing regions of Tabora, Ruvuma, Iringa and Kilimanjaro in Tanzania in order to estimate total fumonisin (FB1 + FB2) exposures to adult individuals in the households. The average daily per capita maize consumption of 771 g, recommended by the Tanzania Food and Nutrition Centre (TFNC) for an adult relying on it as a main meal, and also average daily per capita maize consumptions of 129, 308 and 356 g documented for Tanzania, were used in the exposure estimation. The fumonisins were determined by HPLC using fluorescence detection. Total fumonisins exposure (µg/kg body weight (bw)/day) was determined by multiplying average daily per capita maize consumption (kg) by fumonisin level in maize (µg/kg) from a given household and then dividing by an average bw of an adult of 60 kg. Of the 120 samples, 52% were contaminated with fumonisins at levels of up to 11,048 µg/kg (median; 363 µg/kg). Based on the recommended maize consumption of 771 g/person/day, fumonisin exposures to adult individuals in 38% of the households would exceed the provisional maximum tolerable daily intake (PMTDI) of 2 µg/kg bw, recommended by the Joint FAO/WHO Expert Committee on Food Additives. At the least documented maize consumption of 129 g/person/day, fumonisin exposures in 16% of the households were still above the PMTDI. Reduction of the maize consumption level to 40 g/person/day is an impractical, and reduction of the maximum contamination level to 155 µg/kg is a possibly practical, option for effective minimisation of fumonisin exposures in these communities. A relatively larger study is needed in order to generate comprehensive data for the formulation of appropriate strategies to minimise fumonisin exposures in Tanzania