Comprehensive lung injury pathology induced by mTOR inhibitors

Molecular Targets in Oncology[Abstract] Interstitial lung disease is a rare side effect of temsirolimus treatment in renal cancer patients. Pulmonary fibrosis is characterised by the accumulation of extracellular matrix collagen, fibroblast proliferation and migration, and loss of alveolar gas exchange units. Previous studies of pulmonary fibrosis have mainly focused on the fibro-proliferative process in the lungs. However, the molecular mechanism by which sirolimus promotes lung fibrosis remains elusive. Here, we propose an overall cascade hypothesis of interstitial lung diseases that represents a common, partly underlying synergism among them as well as the lung pathogenesis side effects of mammalian target of rapamycin inhibitors

Some factors affecting the growth of mammalian cells in suspension cultures

SIGLEAvailable from British Library Document Supply Centre-DSC:DX196325 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Taxonomic studies on the Emarginulinae (Mollusca: Gastropoda: Fissurellidae) of southern Africa and Mozambique. Emarginula, Emarginella, Puncturella, Fissurisepta and Rimula

Nineteen species (six new) of the genera Emarginula, Emarginella, Puncturella, Fissurisepta and Rimula are discussed; the last two genera represent new records for the area. Scanning electron micrographs of shell microsculpture, radula and protoconch are given where possible; microsculpture appears to be a particularly useful taxonomie character. New species: Emarginula phrygium, E.vindicaria, Puncturella voraginosa, P. serraticosta, Fissurisepta onychoides and Rimula rhips. New synonyms: Emarginula vadum Barnard, 1963 = E. undulata Melvill & Standen, 1903; Fissurisepta joschristiaensi Drivas & Jay, 1985 = Puncturella christiaensi Kilburn, 1978. Revised combination: oppressa Barnard, 1963, is an Emarginula, not an Emarginella. New record: Puncturella aethiopicavon Martens, 1902, described from the Zanzibar Channel, occurs off Natal and Transkei. Lectotypes designated and figured: Emarginula undulata Melvill & Standen, 1903, and Emarginella sibogae (Schepman, 1908)

Nutrient cycling in early coral life stages:Pocillopora damicornislarvae provide their algal symbiont (Symbiodinium) with nitrogen acquired from bacterial associates

The waters surrounding coral reef ecosystems are generally poor in nutrients, yet their levels of primary production are comparable with those reported from tropical rain forests. One explanation of this paradox is the efficient cycling of nutrients between the coral host, its endosymbiotic alga Symbiodinium and a wide array of microorganisms. Despite their importance for the animals' fitness, the cycling of nutrients in early coral life stages and the initial establishment of partnerships with the microbes involved in these processes has received little scrutiny to date. Nitrogen is an essential but limited nutrient in coral reef ecosystems. In order to assess the early nutrient exchange between bacteria and corals, coral larvae of the species Pocillopora damicornis were incubated with two coral-associated bacteria (Alteromonas sp., or Vibrio alginolyticus), prelabeled with the stable nitrogen isotope N-15. The incorporation and translocation of nitrogen from Vibrio- and Alteromonas bacteria into P. damicornis coral larvae and specifically into the coral-symbiotic Symbiodinium were detected by nanoscale secondary ion mass spectrometry (NanoSIMS). A significant increase in the amount of enriched N-15 (two to threefold compared to natural abundance) was observed in P. damicornis larvae within 8h of incubation for both bacterial treatments (one-way ANOVA, F-5,F-53=18.03, P=0.004 for Alteromonas sp. and F-5,F-53=18.03, P=0.0001 for V. alginolyticus). These findings reveal that coral larvae acquire nutrients previously taken up from the environment by bacteria. The additional nitrogen may increase the survival rate and fitness of the developing coral and therefore contribute to the successful maintenance of coral reefs

Domains in microbial β-1,4-glycanases : sequence conservation, function and enzyme families

International audienc

A novel two-zone protein uptake model for affinity chromatography and its application to the description of elution band profiles of proteins fused to a family 9 cellulose binding module affinity tag

A novel two-zone model (TZM) is presented to describe the rate of solute uptake by the stationary phase of a sorption-type chromatography column. The TZM divides the porous stationary-phase particle into an inner protein-free core and an outer protein-containing zone where intraparticle transport is limited by pore diffusion and binding follows Langmuir theory. The TZM and the classic pore-diffusion model (PDM) of chromatography are applied to the prediction of stationary-phase uptake and elution bands within a cellulose-based affinity chromatography column designed to selectively purify proteins genetically labelled with a CBM9 (family 9 cellulose binding module) affinity tag. Under both linear and nonlinear loading conditions, the TZM closely matches rates of protein uptake within the stationary phase particles as measured by confocal laser scanning microscopy, while the PDM deviates from experiment in the linear-binding region. As a result, the TZM is shown to provide improved predictions of product breakthrough, including elution behavior from a bacterial lysate feed

The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose

International audienc

Visualization of the adsoption of a bacterial endo-β-1-4-glucanase and its isolated cellulose-binding domain to crystalline cellulose

International audienc

Structural and functional relationships in two families of β-1,4-glycanases

International audienc