The Non-Legume Parasponia andersonii Mediates the Fitness of Nitrogen-Fixing Rhizobial Symbionts Under High Nitrogen Conditions

Organisms rely on symbiotic associations for metabolism, protection, and energy. However, these intimate partnerships can be vulnerable to exploitation. What prevents microbial mutualists from parasitizing their hosts? In legumes, there is evidence that hosts have evolved sophisticated mechanisms to manage their symbiotic rhizobia, but the generality and evolutionary origins of these control mechanisms are under debate. Here, we focused on the symbiosis between Parasponia hosts and N2-fixing rhizobium bacteria. Parasponia is the only non-legume lineage to have evolved a rhizobial symbiosis and thus provides an evolutionary replicate to test how rhizobial exploitation is controlled. A key question is whether Parasponia hosts can prevent colonization of rhizobia under high nitrogen conditions, when the contribution of the symbiont becomes nonessential. We grew Parasponia andersonii inoculated with Bradyrhizobium elkanii under four ammonium nitrate concentrations in a controlled growth chamber. We measured shoot and root dry weight, nodule number, nodule fresh weight, nodule volume. To quantify viable rhizobial populations in planta, we crushed nodules and determined colony forming units (CFU), as a rhizobia fitness proxy. We show that, like legumes and actinorhizal plants, P. andersonii is able to control nodule symbiosis in response to exogenous nitrogen. While the relative host growth benefits of inoculation decreased with nitrogen fertilization, our highest ammonium nitrate concentration (3.75 mM) was sufficient to prevent nodule formation on inoculated roots. Rhizobial populations were highest in nitrogen free medium. While we do not yet know the mechanism, our results suggest that control mechanisms over rhizobia are not exclusive to the legume clade.</p

Evaluatie Opvangbeleid 2005-2008 overwinterende ganzen en smienten. Deelrapport 6. Foerageergebieden rond Natura2000-gebieden met ganzendoelstellingen

Dit onderzoek evalueert de effectiviteit van de aanwijzing van foerageergebieden in en rond Natura 2000 gebieden. De beschikbare draagkracht van de foerageergebieden in Natura 2000 gebieden en binnen een afstand van 5, 10 en 15 km is vergeleken met de vereiste draagkracht van deze gebieden. De vergelijking laat zien dat de ganzen op regionaal niveau in staat moeten zijn voldoende foerageer- en rustgebied te vinden binnen de regio. Er is dus in theorie voldoende foerageergebied aanwezig. Dat blijkt uit onderzoek van Alterra, in samenwerking met NIO

Coherence of neutrino flavor mixing in quantum field theory

In the simplistic quantum mechanical picture of flavor mixing, conditions on the maximum size and minimum coherence time of the source and detector regions for the observation of interference---as well as the very viability of the approach---can only be argued in an ad hoc way from principles external to the formalism itself. To examine these conditions in a more fundamental way, the quantum field theoretical $S$-matrix approach is employed in this paper, without the unrealistic assumption of microscopic stationarity. The fully normalized, time-dependent neutrino flavor mixing event rates presented here automatically reveal the coherence conditions in a natural, self-contained, and physically unambiguous way, while quantitatively describing the transition to their failure.Comment: 12 pages, submitted to Phys. Rev.

Tradeoffs in the evolution of plant farming by ants

Diverse forms of cultivation have evolved across the tree of life. Efficient farming requires that the farmer deciphers and actively promotes conditions that increase crop yield. For plant cultivation, this can include evaluating tradeoffs among light, nutrients, and protection against herbivores. It is not understood if, or how, nonhuman farmers evaluate local conditions to increase payoffs. Here, we address this question using an obligate farming mutualism between the ant Philidris nagasau and epiphytic plants in the genus Squamellaria that are cultivated for their nesting sites and floral rewards. We focused on the ants’ active fertilization of their crops and their protection against herbivory. We found that ants benefited from cultivating plants in full sun, receiving 7.5-fold more floral food rewards compared to shade-cultivated plants. The higher reward levels correlated with higher levels of crop protection provided by the ants. However, while high-light planting yielded the greatest immediate food rewards, sun-grown crops contained less nitrogen compared to shade-grown crops. This was due to lower nitrogen input from ants feeding on floral rewards instead of insect protein gained from predation. Despite this tradeoff, farming ants optimize crop yield by selectively planting their crops in full sun. Ancestral state reconstructions across this ant–plant clade show that a full-sun farming strategy has existed for millions of years, suggesting that nonhuman farmers have evolved the means to evaluate and balance conflicting crop needs to their own benefit

«Через край із серця рідне слово ллється…» (про мову поезій П. Куліша)

Several studies have shown that soil biotic communities from organically managed fields are more diverse and exhibit higher activity levels compared to conventionally managed fields. The impact of these different soil communities on plant productivity and the provision of soil ecosystem services are, however, still unclear. Here, we test the effects of soil inoculation from each of three organic and three conventional maize fields on maize productivity and nutrient loss during leaching events induced by simulated rain. In particular, we examine whether differences in productivity and nutrient loss are related to the abundance and species composition of arbuscular mycorrhizal (AM) fungi. We hypothesized that soil biota from organically managed fields would improve maize growth and reduce nutrient leaching significantly more than those from conventionally managed fields. In contrast to our hypothesis, we found that plant productivity was negatively affected by soil inoculation, and this effect was stronger with inoculum from organic fields. Plant productivity was inversely correlated with AMF abundance, suggesting that enhanced carbon allocation to AMF is at least in part responsible for plant growth reduction under our experimental conditions. However, soil inoculation did alter the ecological functioning of the system by reducing phosphorus leaching losses after simulated rain. Moreover, these leaching losses were lower with increased hyphal density and were related with abundance of particular AMF types, suggesting that abundance of AMF and their community composition may be useful indicators of phosphorus leaching losses. The results demonstrate that soil communities from different agricultural fields vary in their impact on plant productivity and nutrient leaching losses. The results further indicate that there is a potential tradeoff between positive effects of soil communities on sustainability and negative effects on crop productivity. © 2011 The Author(s)

Mutualisms drive plant trait evolution beyond interaction‐related traits

Mutualisms have driven the evolution of extraordinary structures and behavioural traits, but their impact on traits beyond those directly involved in the interaction remains unclear. We addressed this gap using a highly evolutionarily replicated system – epiphytes in the Rubiaceae forming symbioses with ants. We employed models that allow us to test the influence of discrete mutualistic traits on continuous non‐mutualistic traits. Our findings are consistent with mutualism shaping the pace of morphological evolution, strength of selection and long‐term mean of non‐mutualistic traits in function of mutualistic dependency. While specialised and obligate mutualisms are associated with slower trait change, less intimate, facultative and generalist mutualistic interactions – which are the most common – have a greater impact on non‐mutualistic trait evolution. These results challenge the prevailing notion that mutualisms solely affect the evolution of interaction‐related traits via stabilizing selection and instead demonstrate a broader role for mutualisms in shaping trait evolution

Adaptation and enslavement in endosymbiont-host associations

The evolutionary persistence of symbiotic associations is a puzzle. Adaptation should eliminate cooperative traits if it is possible to enjoy the advantages of cooperation without reciprocating - a facet of cooperation known in game theory as the Prisoner's Dilemma. Despite this barrier, symbioses are widespread, and may have been necessary for the evolution of complex life. The discovery of strategies such as tit-for-tat has been presented as a general solution to the problem of cooperation. However, this only holds for within-species cooperation, where a single strategy will come to dominate the population. In a symbiotic association each species may have a different strategy, and the theoretical analysis of the single species problem is no guide to the outcome. We present basic analysis of two-species cooperation and show that a species with a fast adaptation rate is enslaved by a slowly evolving one. Paradoxically, the rapidly evolving species becomes highly cooperative, whereas the slowly evolving one gives little in return. This helps understand the occurrence of endosymbioses where the host benefits, but the symbionts appear to gain little from the association.Comment: v2: Correction made to equations 5 & 6 v3: Revised version accepted in Phys. Rev. E; New figure adde

Three heavy jet events at hadron colliders as a sensitive probe of the Higgs sector

Assuming that a non-standard neutral Higgs with an enhanced Yukawa coupling to a bottom quark is observed at future hadron experiments, we propose a method for a better understanding of the Higgs sector. Our procedure is based on "counting" the number of events with heavy jets (where "heavy" stands for a c or b jet) versus b jets, in the final state of processes in which the Higgs is produced in association with a single high p_T c or b jet. We show that an observed signal of the type proposed, at either the Tevatron or the LHC, will rule out the popular two Higgs doublet model of type II as well as its supersymmetric version - the Minimal Supersymmetric Standard Model (MSSM), and may provide new evidence in favor of some more exotic multi Higgs scenarios. As an example, we show that in a version of a two Higgs doublet model which naturally accounts for the large mass of the top quark, our signal can be easily detected at the LHC within that framework. We also find that such a signal may be observable at the upgraded Tevatron RunIII, if the neutral Higgs in this model has a mass around 100 GeV and \tan\beta > 50 and if the efficiency for distinguishing a c jet from a light jet will reach the level of 50%.Comment: Revtex, 11 pages, 4 figures embedded in the text. Main changes with respect to Version 1: Numerical results re-calculated using the CTEQ5L pdf, improved discussion on the experimental consequences, new references added. Conclusions remain unchanged. As will appear in Phys. Rev.