Determination of Temperature-Dependent Elastic Constants of Steel AISI 4140 by Use of In Situ X-ray Dilatometry Experiments

In situ dilatometry experiments using high energy synchrotron X-ray diffraction in transmission mode were carried out at the high energy material science beamline P07@PETRAIII atDESY (Deutsches Elektronen Synchrotron) for the tempering steel AISI 4140 at defined mechanical loading. The focus of this study was on the initial tempering state ( f errite) and the hardened state (martensite). Lattice strains were calculated from the 2D diffraction data for different hkl planes and from those temperature-dependent lattice plane specific diffraction elastic constants (DECs) were determined. The resulting coupling terms allow for precise stress analysis for typical hypoeutectoid steels using diffraction data during heat treatment processes, that is, for in situ diffraction studies during thermal exposure. In addition, by averaging hkl specific Young's moduli and Poisson ratios macroscopic temperature-dependent elastic constants were determined. In conclusion a novel approach for the determination of phase-specific temperature-dependent DECs was suggested using diffraction based dilatometry that provides more reliable data in comparison to conventional experimental procedures. Moreover, the averaging of lattice plane specific results from in situ diffraction analysis supply robust temperature-dependent macroscopic elastic constants for martensite and ferrite as input data for heat treatment process simulations

Action Management – Status, Requirements And Implementation Strategies For SMEs

Due to its great importance for a successful planning, control and improvement of business processes, action management is long established as essential management process in most companies. However, there is often a strikingly large gap between claim and actual implementation of action management. While internal and external requirements for action management are continuously increasing, its actual implementation - especially in small and medium-sized enterprises (SMEs) - is already often quite incomplete today. First, this paper introduces in the topic field of action management as part of modern management systems. In its analysis part, the paper presents the current implementation status of action management in companies focusing on SMEs and portrays software-technical implementation possibilities. Taking into account the resulting fields of action, possible strategies to implement action management in SMEs' business processes in a more profitable way are presented. In the sense of a socio-technical overall system, not only methodical issues but also information-technical and organizational aspects are discussed. By means of a developed prototype and taking into account a concrete use case from industry, the characteristics, procedure, potentials and current limits of the proposed solution are critically evaluated and recommendations for action are illustrated. Finally, the paper ends with a summary, a discussion and an outlook towards future trends

50 Hz X‐Ray Diffraction Stress Analysis and Numerical Process Simulation at Laser Surface Line Hardening of Web Structures

In situ synchrotron X-ray diffraction experiments were carried out during laser surface line hardening of the common tempering steel AISI 4140 at beamline P05@PETRA III operated by Helmholtz-Zentrum Geesthacht at the Deutsches Elektronen Synchrotron, Hamburg, Germany. A unique process chamber was used to investigate the phase and transverse surface stress evolution during a laser line hardening processes. Synchrotron radiation, in combination with microstrip line detectors, allows for a time resolution of 50 Hz. Specimen geometries were hardened using a high-power diode laser under control of the surface temperature and constant laser beam feed. Herein, it is focused on web-structured specimens in contrast to a flat geometry. The experimental results are discussed with regard to the workpiece geometry effect of the web structure dimensions on the temporal and spatial stress evolution. In addition, numerical process simulations based on the finite element method were carried out to support the drawn conclusions. The presented model is able to predict the surface transverse stresses inside the process zone center, while providing further 3D information. A heat build-up in the web leads to a wider and deeper process zone, however, the absolute hardness increase and the transverse residual stresses at the surface center are not affected

Modellierungs-Studie Reparaturkosten-Empfehlung : Bericht an die Verbraucherzentrale

Die Modellierungs-Studie "Reparaturkosten-Empfehlung" wurde vom Wuppertal Institut im Auftrag der Verbraucherzentrale Nordrhein-Westfalen erstellt. Die Studie zielt auf die Berechnung von maximalen Reparaturkosten, die für eine Reparatur aus finanzieller Sicht sinnvoll für Verbraucherinnen und Verbraucher sind. Zusätzlich wird die ökologische Vorteilhaftigkeit von Reparaturen beispielhaft diskutiert

iWindow - Intelligentes Maschinenfenster

Das Verbundforschungsprojekt iWindow: Intelligentes Maschinenfenster beschäftigte sich mit der visuellen Unterstützung von Maschinenbedienern an Werkzeugmaschinen. Diese konnten bisher nur auf wenige bis keine Systeme, die sie bei ihren täglichen Aufgaben direkt an der Werkzeugmaschine unterstützen, zurückgreifen. Das Forschungsprojekt verbindet reale und virtuelle Welt in der Werkzeugmaschine durch Technologien wie Virtual und Augmented Reality, digitaler Zwilling, Simulation und Mehrwertdienste. Durch Nutzung jeweils für die aktuelle Arbeitssituation passender Dienste, werden Mitarbeiter befähigt, sich an die steigende Individualisierung der Produkte und die flexiblere Produktion anzupassen. Kunden und Geschäftspartner werden durch die Möglichkeit eigene mehrwertgenerierende Dienste zu entwickeln und anderen Anwendern zur Verfügung zu stellen in den Wertschöpfungsprozess eingebunden. Diese Publikation beleuchtet die im Rahmen des Forschungsprojekts erarbeiteten Ergebnisse hinsichtlich für ein intelligentes Maschinenfenster benötigter Technologien und Entwicklungen

Metabolite Profiling Uncovers Plasmid-Induced Cobalt Limitation under Methylotrophic Growth Conditions

BACKGROUND:The introduction and maintenance of plasmids in cells is often associated with a reduction of growth rate. The reason for this growth reduction is unclear in many cases. METHODOLOGY/PRINCIPAL FINDINGS:We observed a surprisingly large reduction in growth rate of about 50% of Methylobacterium extorquens AM1 during methylotrophic growth in the presence of a plasmid, pCM80 expressing the tetA gene, relative to the wild-type. A less pronounced growth delay during growth under non-methylotrophic growth conditions was observed; this suggested an inhibition of one-carbon metabolism rather than a general growth inhibition or metabolic burden. Metabolome analyses revealed an increase in pool sizes of ethylmalonyl-CoA and methylmalonyl-CoA of more than 6- and 35-fold, respectively, relative to wild type, suggesting a strongly reduced conversion of these central intermediates, which are essential for glyoxylate regeneration in this model methylotroph. Similar results were found for M. extorquens AM1 pCM160 which confers kanamycin resistance. These intermediates of the ethylmalonyl-CoA pathway have in common their conversion by coenzyme B(12)-dependent mutases, which have cobalt as a central ligand. The one-carbon metabolism-related growth delay was restored by providing higher cobalt concentrations, by heterologous expression of isocitrate lyase as an alternative path for glyoxylate regeneration, or by identification and overproduction of proteins involved in cobalt import. CONCLUSIONS/SIGNIFICANCE:This study demonstrates that the introduction of the plasmids leads to an apparent inhibition of the cobalt-dependent enzymes of the ethylmalonyl-CoA pathway. Possible explanations are presented and point to a limited cobalt concentration in the cell as a consequence of the antibiotic stress

Genetic Contribution to Alcohol Dependence: Investigation of a Heterogeneous German Sample of Individuals with Alcohol Dependence, Chronic Alcoholic Pancreatitis, and Alcohol-Related Cirrhosis

The present study investigated the genetic contribution to alcohol dependence (AD) using genome-wide association data from three German samples. These comprised patients with: (i) AD; (ii) chronic alcoholic pancreatitis (ACP); and (iii) alcohol-related liver cirrhosis (ALC). Single marker, gene-based, and pathway analyses were conducted. A significant association was detected for the ADH1B locus in a gene-based approach (puncorrected = 1.2 × 10−6; pcorrected = 0.020). This was driven by the AD subsample. No association with ADH1B was found in the combined ACP + ALC sample. On first inspection, this seems surprising, since ADH1B is a robustly replicated risk gene for AD and may therefore be expected to be associated also with subgroups of AD patients. The negative finding in the ACP + ALC sample, however, may reflect genetic stratification as well as random fluctuation of allele frequencies in the cases and controls, demonstrating the importance of large samples in which the phenotype is well assessed

Regulation of GDF-15, a distant TGF-β superfamily member, in a mouse model of cerebral ischemia

GDF-15 is a novel distant member of the TGF-β superfamily and is widely distributed in the brain and peripheral nervous system. We have previously reported that GDF-15 is a potent neurotrophic factor for lesioned dopaminergic neurons in the substantia nigra, and that GDF-15-deficient mice show progressive postnatal losses of motor and sensory neurons. We have now investigated the regulation of GDF-15 mRNA and immunoreactivity in the murine hippocampal formation and selected cortical areas following an ischemic lesion by occlusion of the middle cerebral artery (MCAO). MCAO prominently upregulates GDF-15 mRNA in the hippocampus and parietal cortex at 3 h and 24 h after lesion. GDF-15 immunoreactivity, which is hardly detectable in the unlesioned brain, is drastically upregulated in neurons identified by double-staining with NeuN. NeuN staining reveals that most, if not all, neurons in the granular layer of the dentate gyrus and pyramidal layers of the cornu ammonis become GDF-15-immunoreactive. Moderate induction of GDF-15 immunoreactivity has been observed in a small number of microglial cells identified by labeling with tomato lectin, whereas astroglial cells remain GDF-15-negative after MCAO. Comparative analysis of the size of the infarcted area after MCAO in GDF-15 wild-type and knockout mice has failed to reveal significant differences. Together, our data substantiate the notion that GDF-15 is prominently upregulated in the lesioned brain and might be involved in orchestrating post-lesional responses other than the trophic support of neurons

Patterns of Alcohol Consumption Among Individuals With Alcohol Use Disorder During the COVID-19 Pandemic and Lockdowns in Germany

Importance Alcohol consumption (AC) leads to death and disability worldwide. Ongoing discussions on potential negative effects of the COVID-19 pandemic on AC need to be informed by real-world evidence. Objective To examine whether lockdown measures are associated with AC and consumption-related temporal and psychological within-person mechanisms. Design, Setting, and Participants This quantitative, intensive, longitudinal cohort study recruited 1743 participants from 3 sites from February 20, 2020, to February 28, 2021. Data were provided before and within the second lockdown of the COVID-19 pandemic in Germany: before lockdown (October 2 to November 1, 2020); light lockdown (November 2 to December 15, 2020); and hard lockdown (December 16, 2020, to February 28, 2021). Main Outcomes and Measures Daily ratings of AC (main outcome) captured during 3 lockdown phases (main variable) and temporal (weekends and holidays) and psychological (social isolation and drinking intention) correlates. Results Of the 1743 screened participants, 189 (119 [63.0%] male; median [IQR] age, 37 [27.5-52.0] years) with at least 2 alcohol use disorder (AUD) criteria according to the Diagnostic and Statistical Manual of Mental Disorders (Fifth Edition) yet without the need for medically supervised alcohol withdrawal were included. These individuals provided 14 694 smartphone ratings from October 2020 through February 2021. Multilevel modeling revealed significantly higher AC (grams of alcohol per day) on weekend days vs weekdays (β = 11.39; 95% CI, 10.00-12.77; P < .001). Alcohol consumption was above the overall average on Christmas (β = 26.82; 95% CI, 21.87-31.77; P < .001) and New Year’s Eve (β = 66.88; 95% CI, 59.22-74.54; P < .001). During the hard lockdown, perceived social isolation was significantly higher (β = 0.12; 95% CI, 0.06-0.15; P < .001), but AC was significantly lower (β = −5.45; 95% CI, −8.00 to −2.90; P = .001). Independent of lockdown, intention to drink less alcohol was associated with lower AC (β = −11.10; 95% CI, −13.63 to −8.58; P < .001). Notably, differences in AC between weekend and weekdays decreased both during the hard lockdown (β = −6.14; 95% CI, −9.96 to −2.31; P = .002) and in participants with severe AUD (β = −6.26; 95% CI, −10.18 to −2.34; P = .002). Conclusions and Relevance This 5-month cohort study found no immediate negative associations of lockdown measures with overall AC. Rather, weekend-weekday and holiday AC patterns exceeded lockdown effects. Differences in AC between weekend days and weekdays evinced that weekend drinking cycles decreased as a function of AUD severity and lockdown measures, indicating a potential mechanism of losing and regaining control. This finding suggests that temporal patterns and drinking intention constitute promising targets for prevention and intervention, even in high-risk individuals