Molecular phylogeography of the smooth-­‐coated otter (Lutrogale perspicillata, Mustelidae) for its conservation in the Mesopotamian marshes of Iraq

The smooth-coated otter (Lutrogale perspicillata) (SCO) includes perspicillata (SE Asia), sindica (SE Pakistan, W India) and endemic-to-Iraq maxwelli morphological subspecies. Classified as vulnerable by IUCN, the SCO is threatened by habitat transformation, illegal trade and poaching. Non-invasively collected samples in Iraq (10), central and E Asia (nine countries, 46) were sequenced at the entire mtDNA Cytochrome-b gene and genotyped (ongoing) at the microsatellite DNA. We also obtained 307 bp-long gene fragments from 16 SCOs resident to US and European museums. We used 19 Lutrinae GenBank entries. In Iraq, we provided genetic evidence for endemicity of maxwelli to Mesopotamia (private haplotype, mostly from Al-Hawizeh Marsh, S Iraq), while we could not confirm the occurrence of the SCO in N Iraq (Kurdistan).We found reciprocally monophyletic mtDNA lineages across the SCO range each corresponding to a subspecies. Preliminary microsatellite DNA results confirmed this mtDNA picture, overall pointing to three Evolutionarily Significant Units. Looking-like SCOs from Singapore showed oriental small-clawed otter (Aonyx cinerea) mtDNA. We will investigate A. cinerea-mtDNA introgressed SCOs at the microsatellite DNA to assess if they are descendant of L. perspicillata x A. cinerea otters (i.e., first hybrid record in the wild for Lutrinae). Several museum specimens were genetically identified as other than the SCO (even an African otter),indicating that morphology can be not sufficient to reliably catalogue otter study skins. In Iraq, genetic and fieldwork (not shown) data will help establishing a National Single Species Action Plan for the SCO. [National Geographic Society Conservation

A simple compensation method for the accurate measurement of magnetic losses with a single strip tester

International audienceWe present a new method for the accurate characterization of soft magnetic sheets using a permeameter based on the precise compensation of the magnetomotive force (MMF) drop in the flux-closing yoke. It has been developed in order to overcome the systematic uncertainty affecting the value of the magnetic fieldstrength in single sheet testers when obtained, according to the standards, through the measurement of the magnetizing current. This phenomenon is more critical for high permeability materials, because of the reduced MMF drop across the sample. While additional sensors and auxiliary windings have been proposed in the literature, a novel approach is demonstrated here, based on the use of the permeameter upper half yoke as the MMF drop sensor and of an auxiliary winding on the lower half yoke, implementing compensation. This solution, dispensing one from dealing with the usually small signal levels of the conventional MMF drop sensors (e.g. Chattock coils), provides best results with the introduction of wedge-shaped magnetic poles, in order to accurately define the magnetic path length. The method is validated by measurements of power loss, apparent power, and hysteresis cycles on non-oriented and grain-oriented Fe-Si steel sheets, which are compared with local measurements performed on the same samples using H-coil and B-coil across a uniformly magnetized region

Analysis, Design and Implementation of an End-to-End QKD Link

This manuscript discusses the most relevant aspects of the practical implementation of a long-range Quantum Key Distribution (QKD) link with trusted nodes, achieving the highest possible secret key rate generation within the security and system level constraints. To this purpose, we report on recent pilot studies for the measurements of detection efficiency and source photon statistics for validating the calibration facilities (i) at telecom wavelength for realistic quantum backbone implementation through standard telecommunications grade optical fiber, and (ii) for the telecom and VIS-NIR regime. In addition, since there are circumstances when a fiber optical link may not be available, we will also discuss the characterization of a Free Space Optics (FSO) QKD link. Finally, the manuscript also discusses the problem of information reconciliation in Continuous Variable QKD (CV-QKD) scenarios

Continuum quasiparticle random phase approximation and the time dependent Hartree-Fock-Bogoliubov approach

Quadrupole excitations of neutron-rich nuclei are analyzed by using the linear response method in the Quasiparticle Random Phase Approximation (QRPA). The QRPA response is derived starting from the time-dependent Hartree-Fock-Bogoliubov (HFB) equations. The residual interaction between the quasiparticles is determined consistently from the two-body force used in the HFB equations, and the continuum coupling is treated exactly. Calculations are done for the neutron-rich oxygen isotopes. It is found that pairing correlations affect the low-lying states, and that a full treatment of the continuum can change the structure of the states in the giant resonance region.Comment: 17 pages, 7 figures. Revised version with comments and references adde

Prevalence of Muscle Dysmorphia and Associated Health Activities in Male Medical Students in Karachi, Pakistan

Background: Muscle Dysmorphia (MD) is a subtype of body dysmorphic disorder (BDD) and is currently classified under anxiety disorders (subheading: Obsessive-compulsive disorder) in DSM 5. MD is hypothesized to affect the self-esteem and social outlook of the younger generation. MD shows a higher rate in males and may influence their self-confidence rendering them more prone towards using steroids, supplementary proteins and other drugs to alter their physical outlooks as shown in previous studies. This problem has been on the rise lately due to revolutionary advancement in the media and film industry and the abrupt changes about the standards of physical good looks and body shapes. With the lack of studies done in our population, our study will be helpful to consider the prevalence of the disease in our setting and increase awareness in the general public and clinicians. We hope to help clinicians/ therapists find better options in managing the disease. Materials: We performed a cross-sectional study with a sample size of 246 medical school students in Karachi to collect data through self-administered questionnaires. We used the DSM 5 criteria for the diagnosis of BDD and additional questions on the presence of MD. Nutritional habits, exercise routines, use of supplements and drugs were also obtained for exploratory analysis. Results: Our study predicted the prevalence of MD to be 25%. Other main findings included statistical significant associations between MD and the thoughts and practice of steroid use for muscularity. Conclusion: MD is an underdiagnosed and often unrecognized disease that we believe has significant consequences for the young male population. Further work is needed on this in our part of the world. Our research, we believe, can be a stepping stone for further studies that would incorporate wider populations

Spectroscopy of 35^{35}P using the one-proton knockout reaction

The structure of $^{35}$P was studied with a one-proton knockout reaction at88~MeV/u from a $^{36}$S projectile beam at NSCL. The $\gamma$ rays from thedepopulation of excited states in $^{35}$P were detected with GRETINA, whilethe $^{35}$P nuclei were identified event-by-event in the focal plane of theS800 spectrograph. The level scheme of $^{35}$P was deduced up to 7.5 MeV using$\gamma-\gamma$ coincidences. The observed levels were attributed to protonremovals from the $sd$-shell and also from the deeply-bound $p\_{1/2}$ orbital.The orbital angular momentum of each state was derived from the comparisonbetween experimental and calculated shapes of individual ($\gamma$-gated)parallel momentum distributions. Despite the use of different reactions andtheir associate models, spectroscopic factors, $C^2S$, derived from the$^{36}$S $(-1p)$ knockout reaction agree with those obtained earlier from$^{36}$S($d$,\nuc{3}{He}) transfer, if a reduction factor $R\_s$, as deducedfrom inclusive one-nucleon removal cross sections, is applied to the knockout transitions.In addition to the expected proton-hole configurations, other states were observedwith individual cross sections of the order of 0.5~mb. Based on their shiftedparallel momentum distributions, their decay modes to negative parity states,their high excitation energy (around 4.7~MeV) and the fact that they were notobserved in the ($d$,\nuc{3}{He}) reaction, we propose that they may resultfrom a two-step mechanism or a nucleon-exchange reaction with subsequent neutronevaporation. Regardless of the mechanism, that could not yet be clarified, thesestates likely correspond to neutron core excitations in \nuc{35}{P}. Thisnewly-identified pathway, although weak, offers the possibility to selectivelypopulate certain intruder configurations that are otherwise hard to produceand identify.Comment: 5 figures, 1 table, accepted for publication in Physical Review

In vitro studies on antibacterial, antifungal, and cytotoxic properties of Leucas aspera

The leaves of the plant Leucas aspera belonging to the family Lamiaceae was studied to determine the in vitro antibacterial, antifungal, and cytotoxic properties. All extracts showed remarkable antibacterial activity against all of the studied organisms except Escherichia coli. Methanol extract showed stronger activity compared to ethyl acetate and petroleum ether extracts. It showed highest activity against Pseudomonas aeruginosa with zone of inhibition of 15 mm. The standard chloramphenicol did not show any activity against Shigella sonnei. But all the extracts showed moderate activity against this pathogen with zone of inhibition ranging from 10 to 13 mm. None of the extracts has shown any significant antifungal activity against the fungi. Incase of brine shrimp lethality bioassay, methanol extract showed the strongest cytotoxic effect with LC50 value of 4.28 μg/ml which is followed by ethyl acetate extract with LC50 value of 5.36 μg/ml. Thus it can be inferred that this plant may be a potential source of novel bioactive compounds

Survey on solar X-ray flares and associated coherent radio emissions

The radio emission during 201 X-ray selected solar flares was surveyed from 100 MHz to 4 GHz with the Phoenix-2 spectrometer of ETH Zurich. The selection includes all RHESSI flares larger than C5.0 jointly observed from launch until June 30, 2003. Detailed association rates of radio emission during X-ray flares are reported. In the decimeter wavelength range, type III bursts and the genuinely decimetric emissions (pulsations, continua, and narrowband spikes) were found equally frequently. Both occur predominantly in the peak phase of hard X-ray (HXR) emission, but are less in tune with HXRs than the high-frequency continuum exceeding 4 GHz, attributed to gyrosynchrotron radiation. In 10% of the HXR flares, an intense radiation of the above genuine decimetric types followed in the decay phase or later. Classic meter-wave type III bursts are associated in 33% of all HXR flares, but only in 4% they are the exclusive radio emission. Noise storms were the only radio emission in 5% of the HXR flares, some of them with extended duration. Despite the spatial association (same active region), the noise storm variations are found to be only loosely correlated in time with the X-ray flux. In a surprising 17% of the HXR flares, no coherent radio emission was found in the extremely broad band surveyed. The association but loose correlation between HXR and coherent radio emission is interpreted by multiple reconnection sites connected by common field lines.Comment: Solar Physics, in pres

In vitro Metabolic Stability Study of New Cyclen Based Antimalarial Drug Leads Using RP-HPLC and LC-MS/MS

Metabolic stability of the new antimalarial drug leads is determined using Human Liver Microsome (HLM) and specific cytochrome P450 enzyme (CYP2C8) taking the clinically used antimalarial drug chloroquine as a positive control. Experiment is done using standard methods. All the assays were conducted in 0.5 M phosphate buffer at pH 7.4. In general the metabolic reaction was initiated by adding 1 mM NADPH and 0.5 mg of enzyme. Incubations were done with time frequency of 0 hr, 1 hr, and 2 hrs at 37°C and the reactions were terminated by adding acetonitrile in the equal amounts of the assay mixture taken. The samples were centrifuged for 15 minutes at 10,000×g at 4°C and an aliquot of the supernatant was subjected to analysis using HPLC as well as LC-MS to confirm the masses of the drug and/or metabolite(s), if any. While chloroquine was found to be metabolized in a predictable manner by both HLM and CYP2C8, the drug leads were metabolically stable at similar experimental conditions. This study demonstrated that the new drug leads are worth conducting further preclinical evaluations

Behaviour change intervention for smokeless tobacco cessation: its development, feasibility and fidelity testing in Pakistan and in the UK

Background: People of South Asian-origin are responsible for more than three-quarters of all the smokeless tobacco (SLT) consumption worldwide; yet there is little evidence on the effect of SLT cessation interventions in this population. South Asians use highly addictive and hazardous SLT products that have a strong socio-cultural dimension. We designed a bespoke behaviour change intervention (BCI) to support South Asians in quitting SLT and then evaluated its feasibility in Pakistan and in the UK. Methods: We conducted two literature reviews to identify determinants of SLT use among South Asians and behaviour change techniques (BCTs) likely to modify these, respectively. Iterative consensus development workshops helped in selecting potent BCTs for BCI and designing activities and materials to deliver these. We piloted the BCI in 32 SLT users. All BCI sessions were audiotaped and analysed for adherence to intervention content and the quality of interaction (fidelity index). In-depth interviews with16 participants and five advisors assessed acceptability and feasibility of delivering the BCI, respectively. Quit success was assessed at 6 months by saliva/urine cotinine. Results: The BCI included 23 activities and an interactive pictorial resource that supported these. Activities included raising awareness of the harms of SLT use and benefits of quitting, boosting clients’ motivation and self-efficacy, and developing strategies to manage their triggers, withdrawal symptoms, and relapse should that occur. Betel quid and Guthka were the common forms of SLT used. Pakistani clients were more SLT dependent than those in the UK. Out of 32, four participants had undetectable cotinine at 6 months. Fidelity scores for each site varied between 11.2 and 42.6 for adherence to content – maximum score achievable 44; and between 1.4 and 14 for the quality of interaction - maximum score achievable was 14. Interviews with advisors highlighted the need for additional training on BCTs, integrating nicotine replacement and reducing duration of the pre-quit session. Clients were receptive to health messages but most reported SLT reduction rather than complete cessation. Conclusion: We developed a theory-based BCI that was also acceptable and feasible to deliver with moderate fidelity scores. It now needs to be evaluated in an effectiveness trial