Evaluation of androgenic activity of allium cepa on spermatogenesis in the rat

Allium cepa (onion) has a beneficial effect on disease treatment worldwide and has been used since ancient times as a medicinal and food source. Recently several reports have shown that onion has high antioxidant activity. As antioxidants have an essential effect on sperm health parameters, we investigated the effect of the fresh juice of onion bulbs on the spermatogenesis cycle in rats. Wistar male rats (n = 30) were allocated into 3 groups, control (n = 10) and two test groups (each of 10). The animals in the test groups were subdivided into groups of 2 that received fresh onion juice equivalent to 0.5 and 1 g/rat/ /day of fresh onion. The fresh onion juice was administered by gavage for 20 consecutive days. The animals were kept in standard conditions. On the twentieth day, the testes of rats in all groups were removed and sperm was collected from the epididymis and was prepared for analysis. Serum total testosterone significantly increased in all the test groups (p < 0.05), and levels of LH significantly increased only in the group that received the high dose of fresh onion juice (p < 0.05), but the level of FSH did not differ between the experimental and control groups. The percentage of sperm viability and motility in both test groups significantly increased (p < 0.05), but the sperm concentration significantly increased only in the group that received the high dose of freshly extracted onion juice (p < 0.05). It was evident that there was no difference on sperm morphology and testis weight in test groups compared to the control group. In our study, freshly prepared onion juice significantly affected the sperm number, percentage of viability, and motility; it seems that using 4 g/kg of freshly prepared onion juice is effective in sperm health parameters

Investigating the Effect of Mechanical Activation Parameters on Structural Changes and Leaching Rate of Molybdenite Concentrate

AbstractIn this research, mechanical activation (MA) was employed for leaching rate improvement of molybdenite concentrate in nitric acid media. These experiments were performed in two groups: with and without aluminum oxide (alumina). A full factorial design was used for each group of experiments. Leaching rate increment up to 5 times was observed only in 2hours activation procedure. XRD analysis demonstrated structural disordering in activated MoS2. TEM images showed that particle size has been reduced to nanoscale. The initial powder size was 80% between 2-44μm and dropped to about 10nm and 140nm in MA experiments with and without alumina, respectively. This size reduction would be the main reason of leaching rate enhancement which is more achievable in MA in presence of alumina. The results demonstrate that alumina has a motivating effect in activation procedure to achieve a nanostructure molybdenite. Analysis of variance revealed milling speed is the main parameter in MA without alumina, while, ball to powder ratio is the most important factor in MA procedure in presence of alumina on leaching rate

A study on the dependence of structure of multi-walled carbon nanotubes on acid treatment

In the current research, the role of both concentrated nitric acid and ultrasound waves on oxidation of multi-walled carbon nanotubes (MWNTs) was studied. The functionalized MWCNTs were characterized by transmission electron microscopy (TEM), thermogravimetric analyzer, and Fourier transform infrared spectroscopy (FTIR) techniques. It was found that desirable modifications to MWNTs occurred after acid treatment. Carboxylic acid groups were appeared on the side surfaces of MWNTs. FTIR presented the formation of oxygen-containing groups such as C=O and COOH after modification by concentrated nitric acid. The TEM images showed that the aspect ratio of opened MWCNTs was controlled by both ultrasonic waves and acid treatment time. It was also found that the exposure of about 4 h in nitric acid led to the highest removal of the impurities with the least destructive effect

Association between nucleotide mutation of eNOS gene and serum level of vessel expansion factor (VEF) in non-smoker patients with vascular heart disease

Various mutation on endothelial nitric oxide synthase (eNOs) gene cause reduced production of NO, the expansion factor (VEF) and may accelerate the process of atherosclerosis. The study was designed to investigate the frequency of T-786C polymorphism of the gene or nucleotide mutation of eNOS gene in patients suffering from vascular heart disease (VHD) or coronary artery disease (CAD) in North West of Iran. 120 subjects including 60 patients with angiographically diagnosed CAD and 60 age and sex matched CAD-free subjects as control were studied. The levels of Nitric oxide in the samples were measured with the Griess method. The genotype studies were carried out using allele specific PCR. Comparing with the control reduced levels of NO were noticed in the patient group (P&lt;0.05) and significantly high frequency of eNOs -786C genotype was found in CAD patients (P&lt;0.05). The low levels of NO and increased frequency of T-786C polymorphism might be a risk factor in progression of coronary artery disease in the studied subjects.Keywords: Vascular heart disease, endothelial nitric oxide synthase gene, TC 786 SNP, vessel expansion factor (VEF), non-smokerAfrican Journal of Biotechnology Vol. 12(20), pp. 3023-302

Nonparametric Data Assimilation Scheme for Land Hydrological Applications

Data assimilation, which relies on explicit knowledge of dynamical models, is a well-known approach that addresses models' limitations due to various reasons, such as errors in input and forcing data sets. This approach, however, requires intensive computational efforts, especially for high-dimensional systems such as distributed hydrological models. Alternatively, data-driven methods offer comparable solutions when the physics underlying the models are unknown. For the first time in a hydrological context, a nonparametric framework is implemented here to improve model estimates using available observations. This method uses Takens delay coordinate method to reconstruct the dynamics of the system within a Kalman filtering framework, called the Kalman-Takens filter. A synthetic experiment is undertaken to fully investigate the capability of the proposed method by comparing its performance with that of a standard assimilation framework based on an adaptive unscented Kalman filter (AUKF). Furthermore, using terrestrial water storage (TWS) estimates obtained from the Gravity Recovery And Climate Experiment mission, both filters are applied to a real case scenario to update different water storages over Australia. In situ groundwater and soil moisture measurements within Australia are used to further evaluate the results. The Kalman-Takens filter successfully improves the estimated water storages at levels comparable to the AUKF results, with an average root-mean-square error reduction of 37.30% for groundwater and 12.11% for soil moisture estimates. Additionally, the Kalman-Takens filter, while reducing estimation complexities, requires a fraction of the computational time, that is, ~8 times faster compared to the AUKF approach

Discontinuous Phase Transition in an Exactly Solvable One-Dimensional Creation-Annihilation System

An exactly solvable reaction-diffusion model consisting of first-class particles in the presence of a single second-class particle is introduced on a one-dimensional lattice with periodic boundary condition. The number of first-class particles can be changed due to creation and annihilation reactions. It is shown that the system undergoes a discontinuous phase transition in contrast to the case where the density of the second-class particles is finite and the phase transition is continuous.Comment: Revised, 8 pages, 1 EPS figure. Accepted for publication in Journal of Statistical Mechanics: theory and experimen

The Macroscopic Rate of Nucleic Acid Translocation by Hepatitis C virus Helicase NS3h is Dependent on Both the Sugar and Base Moieties

The NS3 helicase (NS3h) of hepatitis C virus (HCV) is a 3′ to 5′ SF2 RNA and DNA helicase that is essential for the replication of HCV. We have examined the kinetic mechanism of translocation of NS3h along single-stranded nucleic acid with bases rU, dU and dT and have found that the macroscopic rate of translocation is dependent upon both the base and sugar moieties of the nucleic acid, with approximate macroscopic translocation rates of 3 nt/s (oligo-dT), 35 nt/s (oligo-dU), and 42 nt/s (oligo-rU), respectively. We found a strong correlation between the macroscopic translocation rates and the binding affinity of the translocating NS3h protein to the respective substrates such that weaker affinity corresponded to faster translocation. The values of K0.5 for NS3h translocation at a saturating ATP concentration are: (3.3 ± 0.4) μM nucleotide (poly-dT), (27 ± 2) μM nucleotide (poly-dU), and (36 ± 2) μM nucleotide (poly-rU). Furthermore, the results of isothermal titration of NS3h with these oligonucleotides suggest that differences in TΔS° are the principal source of the differences in the affinity of NS3h binding to these substrates. Interestingly, despite the differences in macroscopic translocation rates and binding affinities, the ATP coupling stoichiometry for NS3h translocation was identical for all three substrates, ~0.5 ATP molecules consumed per nucleotide translocated. This similar periodicity of ATP consumption implies a similar mechanism for NS3h translocation along RNA and DNA substrates

Psychometric Properties of the Farsi Version of Posttraumatic Growth Inventory for Children-Revised in Iranian Children with Cancer

Objective: Coping with childhood cancer, as a stressful incident, can lead to a growth in various aspects of the child's life. Therefore, this study aims to validate Posttraumatic Growth Inventory for Children-Revised (PTGI-C-R) in children with cancer. Methods: This methodological research was carried out in referral children hospitals in Tehran. PTGI-C-R was translated and back-translated. Content and face validity were assessed. Confirmatory factor analysis (CFA) was performed on 200 children with inclusion criteria, using LISREL V8.5. Due to the rejection of the model, an exploratory factor analysis (EFA) was done, using SPSS V21. The correlation of posttraumatic growth (PTG) with the variables, i.e., age and gender, was investigated. Results: Some writing changes were made in phrases in the sections concerning face and content validity. CFA rejected the five-factor model due to the undesirable fit indices. Therefore, an EFA was used and the three-factor model was not approved, either despite the statistical appropriateness or due to the lack of similarity between the items loaded on factors. The results also indicated a significant relationship between PTG and age (r = 0.13, P = 0.05). There is no significant relationship between PTG and gender (z = -1.35, P = 0.83). Conclusions: PTGI-C-R does not have desirable psychometric properties in Iranian children with cancer and may not be able to reflect all the aspects of PTG experienced by them. Therefore, it cannot be used as an appropriate scale, and it is necessary to develop and validate a specific tool through a qualitative study. © 2021 Wolters Kluwer Medknow Publications. All rights reserved

Nonequilibrium Steady States of Matrix Product Form: A Solver's Guide

We consider the general problem of determining the steady state of stochastic nonequilibrium systems such as those that have been used to model (among other things) biological transport and traffic flow. We begin with a broad overview of this class of driven diffusive systems - which includes exclusion processes - focusing on interesting physical properties, such as shocks and phase transitions. We then turn our attention specifically to those models for which the exact distribution of microstates in the steady state can be expressed in a matrix product form. In addition to a gentle introduction to this matrix product approach, how it works and how it relates to similar constructions that arise in other physical contexts, we present a unified, pedagogical account of the various means by which the statistical mechanical calculations of macroscopic physical quantities are actually performed. We also review a number of more advanced topics, including nonequilibrium free energy functionals, the classification of exclusion processes involving multiple particle species, existence proofs of a matrix product state for a given model and more complicated variants of the matrix product state that allow various types of parallel dynamics to be handled. We conclude with a brief discussion of open problems for future research.Comment: 127 pages, 31 figures, invited topical review for J. Phys. A (uses IOP class file