51 research outputs found
Mind-modelling with corpus stylistics in David Copperfield
We suggest an innovative approach to literary discourse by using corpus linguistic methods to address research questions from cognitive poetics. In this article, we focus on the way that readers engage in mind-modelling in the process of characterisation. The article sets out our cognitive poetic model of characterisation that emphasises the continuity between literary characterisation and real-life human relationships. The model also aims to deal with the modelling of the author’s mind in line with the modelling of the minds of fictional characters. Crucially, our approach to mind-modelling is text-driven. Therefore we are able to employ corpus linguistic techniques systematically to identify textual patterns that function as cues triggering character information. In this article, we explore our understanding of mind-modelling through the characterisation of Mr. Dick from David Copperfield by Charles Dickens. Using the CLiC tool (Corpus Linguistics in Cheshire) developed for the exploration of 19th-century fiction, we investigate the textual traces in non-quotations around this character, in order to draw out the techniques of characterisation other than speech presentation. We show that Mr. Dick is a thematically and authorially significant character in the novel, and we move towards a rigorous account of the reader’s modelling of authorial intention
Using formative research to develop the healthy eating component of the CHANGE! school-based curriculum intervention
Background: Childhood obesity is a significant public health concern. Many intervention studies have attempted
to combat childhood obesity, often in the absence of formative or preparatory work. This study describes the
healthy eating component of the formative phase of the Children’s Health Activity and Nutrition: Get Educated!
(CHANGE!) project. The aim of the present study was to gather qualitative focus group and interview data
regarding healthy eating particularly in relation to enabling and influencing factors, barriers and knowledge in
children and adults (parents and teachers) from schools within the CHANGE! programme to provide populationspecific
evidence to inform the subsequent intervention design.
Methods: Semi-structured focus group interviews were conducted with children, parents and teachers across 11
primary schools in the Wigan borough of North West England. Sixty children (N = 24 boys), 33 parents (N = 4 male)
and 10 teachers (N = 4 male) participated in the study. Interview questions were structured around the PRECEDE
phases of the PRECEDE-PROCEED model. Interviews were transcribed verbatim and analysed using the pen-profiling
technique.
Results: The pen-profiles revealed that children’s knowledge of healthy eating was generally good, specifically
many children were aware that fruit and vegetable consumption was ‘healthy’ (N = 46). Adults’ knowledge was also
good, including restricting fatty foods, promoting fruit and vegetable intake, and maintaining a balanced diet. The
important role parents play in children’s eating behaviours and food intake was evident. The emerging themes
relating to barriers to healthy eating showed that external drivers such as advertising, the preferred sensory
experience of “unhealthy” foods, and food being used as a reward may play a role in preventing healthy eating.
Conclusions: Data suggest that; knowledge related to diet composition was not a barrier per se to healthy eating,
and education showing how to translate knowledge into behavior or action is required. The key themes that
emerged through the focus groups and pen-profiling data analysis technique will be used to inform and tailor the
healthy eating component of the CHANGE! intervention study.
Trial registration: Current Controlled Trials ISRCTN03863885
Keywords: Nutrition, Childhood obesity, Pen-profiles, Health, School
Prognostic model to predict postoperative acute kidney injury in patients undergoing major gastrointestinal surgery based on a national prospective observational cohort study.
Background: Acute illness, existing co-morbidities and surgical stress response can all contribute to postoperative acute kidney injury (AKI) in patients undergoing major gastrointestinal surgery. The aim of this study was prospectively to develop a pragmatic prognostic model to stratify patients according to risk of developing AKI after major gastrointestinal surgery. Methods: This prospective multicentre cohort study included consecutive adults undergoing elective or emergency gastrointestinal resection, liver resection or stoma reversal in 2-week blocks over a continuous 3-month period. The primary outcome was the rate of AKI within 7 days of surgery. Bootstrap stability was used to select clinically plausible risk factors into the model. Internal model validation was carried out by bootstrap validation. Results: A total of 4544 patients were included across 173 centres in the UK and Ireland. The overall rate of AKI was 14·2 per cent (646 of 4544) and the 30-day mortality rate was 1·8 per cent (84 of 4544). Stage 1 AKI was significantly associated with 30-day mortality (unadjusted odds ratio 7·61, 95 per cent c.i. 4·49 to 12·90; P < 0·001), with increasing odds of death with each AKI stage. Six variables were selected for inclusion in the prognostic model: age, sex, ASA grade, preoperative estimated glomerular filtration rate, planned open surgery and preoperative use of either an angiotensin-converting enzyme inhibitor or an angiotensin receptor blocker. Internal validation demonstrated good model discrimination (c-statistic 0·65). Discussion: Following major gastrointestinal surgery, AKI occurred in one in seven patients. This preoperative prognostic model identified patients at high risk of postoperative AKI. Validation in an independent data set is required to ensure generalizability
Cardio-respiratory development in bird embryos: new insights from a venerable animal model
Nucleotide sequence analysis of the cat gene of Proteus mirabilis: comparison with the type I (Tn9) cat gene.
In Proteus mirabilis PM13 chloramphenicol resistance is mediated by the cat gene, a single copy of which is present in both resistant and sensitive isolates and which reverts at a high frequency. RNA measurements show an about 8.5-fold increase in cat-specific mRNA in cells expressing the resistance phenotype as compared with those which are sensitive to chloramphenicol. DNA sequence analysis has revealed a high degree of homology between the P. mirabilis cat gene and the type I cat variant (Tn9), 76% at the amino acid level and 73% when nucleotides in the coding sequence are compared. Sequence homology between the strain PM13 cat variant and Tn9 cat was not apparent however in the 5' and 3' flanking regions. Segments of near identity were seen when the upstream sequence of the cat of P. mirabilis was compared with the 5' regions of the Salmonella typhimurium flagellin genes H1 and H2, which are alternately expressed by a flip-flop control mechanism involving an invertible promoter and a trans-acting product
Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.
A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules
Nucleotide sequence encoding the biosynthetic dehydroquinase function of the penta-functional arom locus of Aspergillus nidulans.
The nucleotide sequence of a 1.9 Kb HindIII fragment of DNA derived from the arom locus of A.nidulans and encoding the biosynthetic dehydroquinase activity has been determined. The sequences encoding the biosynthetic and catabolic dehydroquinase enzymes of A.nidulans show no detectable homology, strongly suggesting convergent evolutionary pathways. The messenger RNA specified by the arom locus was detected as a 5.3 Kb RNA species
The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.
The AROM locus of A. nidulans, which governs five consecutive steps in pre-chorismate aromatic amino acid biosynthesis, has been cloned in a bacteriophage vector. The nucleotide sequence of the locus reveals a single, open reading-frame of 4,812 base-pairs, apparently without introns. An internal segment of the A. nidulans AROM sequence has extensive homology with the E. coli aroA gene that encodes the 5-enolpyruvylshikimate 3-phosphate synthase
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