10,194 research outputs found
Expression of the insulin-like growth factor-II/mannose-6-phosphate receptor in multiple human tissues during fetal life and early infancy
The insulin like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor has been detected in many cells and tissues. In the rat, there is a dramatic developmental regulation of IGF-II/M6P receptor expression, the receptor being high in fetal and neonatal tissues and declining thereafter. We have systematically studied the expression of the human IGF-II/M6P receptor protein in tissues from 10 human fetuses and infants (age 23 weeks gestation to 24 months postnatal). We have asked 1) whether there is differential expression among different organs, and 2) whether or not the human IGF-II/M6P receptor is developmentally regulated from 23 weeks gestation to 24 months postnatal. Protein was extracted from human tissues using a buffer containing 2% sodium dodecyl sulfate and 2% Triton X-100. Aliquots of the protein extracts were analyzed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and immunoblotting using an anti-IGF- II/M6P receptor antiserum (no. 66416) and 125I-protein A or an immunoperoxidase stain. IGF-II/M6P receptor immunoreactivity was detected in all tissues studied with the highest amount of receptor being expressed in heart, thymus, and kidney and the lowest receptor content being measured in brain and muscle. The receptor content in ovary, testis, lung, and spleen was intermediate. The apparent molecular weight of the IGF-II/M6P receptor (220,000 kilos without reduction of disulfide bonds) varied among the different tissues: in brain the receptor was of lower molecular weight than in other organs. Immunoquantitation experiments employing 125I-protein A and protein extracts from human kidney at different ages revealed a small, albeit not significant, difference of the receptor content between fetal and postnatal tissues: as in other species, larger amounts of receptor seemed to be present in fetal than in postnatal organs. In addition, no significant difference of the receptor content between human fetal liver and early postnatal liver was measured employing 125I-protein A- immunoquantitation in three fetal and five postnatal liver tissue samples. The distribution of IGF-binding protein (IGEBP) species, another abundant and major class of IGF binding principles, was also measured in human fetal and early postnatal lung, liver, kidney, muscle, and brain using Western ligand blotting with 125I-IGF-II: as with IGF-II/M6P receptor immunoreactivity there was differential expression of the different classes of IGFBPs in the various organs
Monte Carlo direct simulation technique user's manual
User manual for Monte Carlo direct simulation techniqu
Mott scattering of polarized electrons in a strong laser field
We present analytical and numerical results of the relativistic calculation
of the transition matrix element and differential cross section for
Mott scattering of initially polarized Dirac particles (electrons) in the
presence of strong laser field with linear polarization. We use exact
Dirac-Volkov wave functions to describe the dressed electrons and the collision
process is treated in the first Born approximation. The influence of the laser
field on the degree of polarization of the scattered electron is reported.Comment: 12 pages, 11 figures, Revte
Detection of Far-Infrared Water Vapor, Hydroxyl, and Carbon Monoxide Emissions from the Supernova Remnant 3C 391
We report the detection of shock-excited far-infrared emission of H2O, OH,
and CO from the supernova remnant 3C 391, using the ISO Long-Wavelength
Spectrometer. This is the first detection of thermal H2O and OH emission from a
supernova remnant. For two other remnants, W~28 and W~44, CO emission was
detected but OH was only detected in absorption. The observed H2O and OH
emission lines arise from levels within ~400 K of the ground state, consistent
with collisional excitation in warm, dense gas created after the passage of the
shock front through the dense clumps in the pre-shock cloud. The post-shock gas
we observe has a density ~2x10^5 cm^{-3} and temperature 100-1000 K, and the
relative abundances of CO:OH:H2O in the emitting region are 100:1:7 for a
temperature of 200 K. The presence of a significant column of warm H2O suggests
that the chemistry has been significantly changed by the shock. The existence
of significant column densities of both OH and H2O, which is at odds with
models for non-dissociative shocks into dense gas, could be due to
photodissociation of H2O or a mix of fast and slow shocks through regions with
different pre-shock density.Comment: AASTeX manuscript and 4 postscript figure
Two-finger selection theory in the Saffman-Taylor problem
We find that solvability theory selects a set of stationary solutions of the
Saffman-Taylor problem with coexistence of two \it unequal \rm fingers
advancing with the same velocity but with different relative widths
and and different tip positions. For vanishingly small
dimensionless surface tension , an infinite discrete set of values of the
total filling fraction and of the relative
individual finger width are selected out of a
two-parameter continuous degeneracy. They scale as
and . The selected values of differ from
those of the single finger case. Explicit approximate expressions for both
spectra are given.Comment: 4 pages, 3 figure
Microscopic Selection of Fluid Fingering Pattern
We study the issue of the selection of viscous fingering patterns in the
limit of small surface tension. Through detailed simulations of anisotropic
fingering, we demonstrate conclusively that no selection independent of the
small-scale cutoff (macroscopic selection) occurs in this system. Rather, the
small-scale cutoff completely controls the pattern, even on short time scales,
in accord with the theory of microscopic solvability. We demonstrate that
ordered patterns are dynamically selected only for not too small surface
tensions. For extremely small surface tensions, the system exhibits chaotic
behavior and no regular pattern is realized.Comment: 6 pages, 5 figure
Mid-Infrared Emission from E+A Galaxies in the Coma Cluster
We have used ISO to observe at 12m seven E+A galaxies plus an additional
emission line galaxy, all in the Coma cluster. E+A galaxies lacking narrow
emission lines have 2.2m to 12m flux density ratios or limits similar
to old stellar populations (typical of early-type galaxies). Only galaxies with
emission lines have enhanced 12m flux density. Excess 12m emission is
therefore correlated with the presence of on-going star formation or an active
galactic nucleus (AGN).
By comparing the current star formation rates with previous rates estimated
from the Balmer absorption features, we divide the galaxies into two groups:
those for which star formation has declined significantly following a dramatic
peak 1 Gyr ago; and those with a significant level of ongoing star
formation or/and an AGN. There is no strong difference in the spatial
distribution on the sky between these two groups. However, the first group has
systemic velocities above the mean cluster value and the second group below
that value. This suggests that the two groups differ kinematically.
Based on surveys of the Coma cluster in the radio, the IRAS sources, and
galaxies detected in H emission, we sum the far infrared luminosity
function of galaxies in the cluster. We find that star formation in late type
galaxies is probably the dominant component of the Coma cluster far infrared
luminosity. The presence of significant emission from intracluster dust is not
yet firmly established. The member galaxies also account for most of the far
infrared output from nearby rich clusters in general.Comment: AAS Latex, accepted for publication in Ap
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