96 research outputs found

    Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination

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    In this paper the operation of an amperometric biosensor producing a chemically amplified signal is modelled numerically. The chemical amplification is achieved by using synergistic substrates. The model is based on non-stationary reaction-diffusion equations. The model involves three layers (compartments): a layer of enzyme solution entrapped on the electrode surface, a dialysis membrane covering the enzyme layer and an outer diffusion layer which is modelled by the Nernst approach. The equation system is solved numerically by using the finite difference technique. The biosensor response and sensitivity are investigated by altering the model parameters influencing the enzyme kinetics as well as the mass transport by diffusion. The biosensor action was analyzed with a special emphasis to the effect of the chemical amplification. The simulation results qualitatively explain and confirm the experimentally observed effect of the synergistic substrates conversion on the biosensor response

    Mottes et châteaux du département du Finistère

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    Plusieurs inventaires ont été consacrés aux mottes du département du Finistère correspondant, pour l’essentiel, aux anciens comtés de Cornouaille, au centre et au sud, et de Léon, au nord. Cependant, ces travaux se limitent trop souvent aux seules mottes, à l’exclusion des autres ouvrages terroyés, des châteaux de pierre et des maisons fortes avec lesquels elles sont parfois confondues. Une prospection thématique publiée en 1997 donne les chiffres suivants : 91 mottes certaines, 91 mottes pro..

    Identification of kinetic parameters in a biochemical system

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