Supercritical fluid explosion process to aid fractionation of lipids from biomass

Disclosed are processes for development and recovery of lipids from biomass. A plant or microorganism-based biomass can be developed to encourage a desired lipid profile. Following development, ecologically friendly normally gaseous fluids such as carbon dioxide can be pressurized to a supercritical state followed by rapid expansion. The fluid is first contacted with a biomass source including oil-containing microorganisms and/or agricultural products. For instance, fungi or algae can be bioconverted from another biomass sources such as canola seed or corn syrup and then contacted with the high pressure fluid. During a contact period, the fluid can diffuse into the biomass, and in particular through the cell walls of the biomass. The fluid undergoes rapid release of pressure and opens the cell structure for improved release of oil. The fluid can optionally be utilized for extraction following the explosion process. For instance, the fluid can be re-pressurized in the same vessel for extraction processes

Integrated Bioprocessing of \u3ci\u3ePythium irregulare\u3c/i\u3e to Obtain PUFA-Rich Oil

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There’s a New Biofuel Crop in Town

Work by Agricultural Research Service scientists in Florence, South Carolina, suggests that farmers in the Southeast could use the tropical legume sunn hemp (Crotalaria juncea) in their crop rotations by harvesting the fast-growing annual for biofuel. Agricultural engineer Keri Cantrell, agronomist Philip Bauer, and environmental engineer Kyoung Ro all work at the ARS Coastal Plains Soil, Water, and Plant Research Center in Florence. They compared the energy content of sunn hemp with cowpea (Vigna unguiculata)—another common regional summer cover crop—in 2004 and 2006. The crops were grown in experimental plots near Florence, and both were harvested on the same day, three times in each study year. The last harvest in both years was conducted right after the first killing freeze of the season

Influence of Corn Residue Harvest Management on Grain, Stover, and Energy Yields

Economic, environmental, and energy independence issues are contributing to rising fossil fuel prices, petroleum supply concerns, and a growing interest in biomass feedstocks as renewable energy sources. Potential feedstocks include perennial grasses, timber, and annual grain crops with our focus being on corn (Zea mays L.) stover. A plot-scale study evaluating stover removal was initiated in 2008 on a South Carolina Coastal Plain Coxville/Rains–Goldsboro– Lynchburg soil association site. In addition to grain and stover yields, carbon balance, greenhouse gas (GHG) emissions and soil quality impact reported elsewhere in this issue, variation in gross energy distribution within various plant fractions — whole plant, below ear shank (bottom), above ear shank (top), cob, as well as leaves and stems of the bottomand top portions (n(part, year)=20) was measured with an isoperibol calorimeter. Stalks from above the ear shank were the most energy dense, averaging 18.8 MJ/kg db, and when combined with other plant parts from above the ear shank, the entire top half was more energy dense than the bottom half — 18.4 versus 18.2 MJ/kg db. Gross energy content of the whole plant, including the cob, averaged 18.28±0.76 MJ/kg db. Over the 4 years, partial to total removal (i.e., 25%to 100 %) of above ground plant biomass could supply between 30 and 168 GJ/ha depending upon annual rainfall. At 168 GJ/ha, the quantity of corn stover biomass (whole plant) available in a 3,254-km2 area (32 km radius) around the study site could potentially support a 500-MW power plant

Remediation of Deep Vadose Zone Radionuclide and Metal Contamination: Status and Issues

This report documents the results of a PNNL literature review to report on the state of maturity of deep vadose zone remediation technologies for metal contaminants including some radionuclides. Its recommendations feed into decisionmakers need for scientific information and cost-effective in situ remediation technlogies needed under DOE's Environmental Management initiative Enhanced Remediation Methods: Scientific & Technical Basis for In Stu Treatment Systems for Metals and Radionuclides

Distribution of Structural Carbohydrates in Corn Plants Across the Southeastern USA

Quantifying lignin and carbohydrate composition of corn (Zea mays L.) is important to support the emerging cellulosic biofuels industry. Therefore, field studies with 0 or 100 % stover removal were established in Alabama and South Carolina as part of the Sun Grant Regional Partnership Corn Stover Project. In Alabama, cereal rye (Secale cereale L.) was also included as an additional experimental factor, serving as a winter cover crop. Plots were located on major soil types representative of their respective states: Compass and Decatur soils in Alabama and a Coxville/Rains-Goldsboro-Lynchburg soil association in South Carolina. Lignin and structural carbohydrate concentrations in the whole (above-ground) plant, cobs, vegetation excluding cobs above the primary ear (top), vegetation below the primary ear (bottom), and vegetation from above the primary ear including cobs (above-ear fraction) were determined using near-infrared spectroscopy (NIRS). The distribution of lignin, ash, and structural carbohydrates varied among plant fractions, but neither inclusion of a rye cover crop nor the stover harvest treatments consistently affected carbohydrate concentrations within locations. Total precipitation and average air temperature during the growing season were strongly correlated with stover composition indicating that weather conditions may have multiple effects on potential biofuel production (i.e., not only yield but also stover quality). When compared to the above-ear fractions, bottom plant partitions contained greater lignin concentrations. Holocellulose concentration was consistently greater in the above-ear fractions at all three locations. Data from this study suggests that the above-ear plant portions have the most desirable characteristics for cellulosic ethanol production via fermentation in the southeastern USA

Increasing biomass of winter wheat using sorghum biochars

International audienceBiochar is a black solid formed by pyrolysis of biomass such as crop residues. Biochar could be used for soil fertilization, carbon sequestration, and improvement of soil structure. Here, we tested the effect of sorghum biochars on winter wheat, with or without supplemental inorganic phosphorus, in a greenhouse. The application rate for sorghum residues and sorghum biochars based on a yield goal of 200 bushels ha−1 was 13 Mg ha−1. Inorganic phosphorus was added at the rate of 40 kg P ha−1. Results show that addition of sorghum biochars increased the total biomass of winter wheat grown by about 31 % over the control plants. Addition of supplemental inorganic phosphorus did not increase the total biomass. Our findings suggest that the pyrolitic transformation of sorghum residues into sorghum biochars is a better strategy for both environmental and crop productivity improvement in the Coastal Plains region